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Volume 23, Number 11—November 2017
Dispatch

High-Level Fosfomycin Resistance in Vancomycin-Resistant Enterococcus faecium

Yan Guo, Adam D. Tomich, Christi L. McElheny, Vaughn S. Cooper, Amelia Tait-Kamradt, Min Chen, Fupin Hu, Louis B. Rice, Nicolas Sluis-Cremer, and Yohei DoiComments to Author 
Author affiliations: University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA (Y. Guo, A.D. Tomich, C.L. McElheny, V.S. Cooper, N. Sluis-Cremer, Y. Doi); Fudan University Huashan Hospital, Shanghai, China (Y. Guo, M. Wang, F. Hu); Brown University, Providence, Rhode Island, USA (A. Tait-Kamradt, L.B. Rice); Fujita Health University, Aichi, Japan (Y. Doi)

Main Article

Table

Michaelis-Menten steady-state kinetic parameters for vancomycin-resistant Enterococcus faecium wild-type and C119D MurA*

Enzyme UNAG (p value)
 PEP (p value)
Km, μmol/L Vmax, μmol/L/min kcat/min kcat/Km, μmol/L/min Km, μmol/L Vmax, μmol/min kcat/min kcat/Km, μmol/L/min
WT MurA 382.8 ± 79.5 13.9 ± 1.6 138.7 ± 16.4 0.4 229.0 ± 87.201 29.5 ± 8.4 294.5 ± 83.8 1.3
CD119D MurA 803.2 ± 1,780 (0.02) 11.9 ± 2.2 (NS) 119.4 ± 22.2 (NS) 0.2 304.6 ± 35.2 (NS) 28.6 ± 3.2 (NS) 285.5 ± 32.1 (NS) 0.9

*Values are mean ± SD for >3 independent experiments unless otherwise indicated. Statistical differences between kinetic parameters for vancomycin-resistant enterococci WT and C119D MurA were assessed by using a paired t-test. MurA, UDP-N-acetylglucosamine enolpyruvyl transferase; NS, not significant; PEP, phosphoenolpyruvate; UNAG, UDP-N-acetylglucosamine; WT, wild type.

Main Article

Page created: October 17, 2017
Page updated: October 17, 2017
Page reviewed: October 17, 2017
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