Figure 2. Immunohistochemical detection of disease-associated prion protein (PrPSc) in a cow at 88 months after oral inoculation with brain homogenate of L-type bovine spongiform encephalopathy agent. A) Low amount of PrPSc deposition in the dorsal motor nucleus of the vagus nerve (DMNV) compared with the more pronounced depositions in the solitary tract nucleus (NST), the spinal tract of trigeminal nerve (STN), and the reticular formation (RFM) in the medulla oblongata at the obex level. Scale bar indicates 200 μm. B) Conspicuous PrPSc deposition in the gray matter of spinal cord (L6). Scale bar indicates 2 mm. C) Granular staining in the neuropil and perineuronal staining (arrows) in the oculomotor nucleus of the midbrain. Scale bar indicates 50 μm. D) Granular PrPSc deposition in the muscle spindle of the ocular muscle. Scale bar indicates 20 μm. E–H) PrPSc in the trigeminal (E), dorsal root (F), cervical cranial (G), and nodose ganglion (H). Scale bars indicate 50 μm.