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Volume 23, Number 3—March 2017


Genetically Diverse Filoviruses in Rousettus and Eonycteris spp. Bats, China, 2009 and 2015




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Xing-Lou Yang1, Yun-Zhi Zhang1, Ren-Di Jiang1, Hua Guo, Wei Zhang, Bei Li, Ning Wang, Li Wang, Cecilia Waruhiu, Ji-Hua Zhou, Shi-Yue Li, Peter Daszak, Lin-Fa Wang, and Zheng-Li ShiComments to Author 
Author affiliations: Chinese Academy of Sciences, Wuhan, China (X.-L. Yang, R.-D. Jiang, H. Guo, W. Zhang, B. Li, N. Wang, L. Wang, C. Waruhiu, Z.-L. Shi); Dali University, Dali, China (Y.-Z. Zhang); University of Chinese Academy of Sciences, Beijing, China (R.-D Jiang, H. Guo, N. Wang); Yunnan Institute of Endemic Diseases Control and Prevention, Dali (Y.-Z. Zhang, J.-H. Zhou); Wuhan University, Wuhan (S.-Y. Li); EcoHealth Alliance, New York, New York, USA (P. Daszak); Duke–NUS Graduate Medical School, Singapore (L.-F. Wang)

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EID Yang X, Zhang Y, Jiang R, Guo H, Zhang W, Li B, et al. Genetically Diverse Filoviruses in Rousettus and Eonycteris spp. Bats, China, 2009 and 2015. Emerg Infect Dis. 2017;23(3):482-486.
AMA Yang X, Zhang Y, Jiang R, et al. Genetically Diverse Filoviruses in Rousettus and Eonycteris spp. Bats, China, 2009 and 2015. Emerging Infectious Diseases. 2017;23(3):482-486. doi:10.3201/eid2303.161119.
APA Yang, X., Zhang, Y., Jiang, R., Guo, H., Zhang, W., Li, B....Shi, Z. (2017). Genetically Diverse Filoviruses in Rousettus and Eonycteris spp. Bats, China, 2009 and 2015. Emerging Infectious Diseases, 23(3), 482-486.


Genetically divergent filoviruses detected in Rousettus and Eonycteris spp. bats in China exhibited 61%–99% nt identity with reported filoviruses, based on partial replicase sequences, and they demonstrated lung tropism. Co-infection with 4 different filoviruses was found in 1 bat. These results demonstrate that fruit bats are key reservoirs of filoviruses.

Filoviruses (family Filoviridae) are nonsegmented, negative-strand RNA viruses belonging to 3 genera: Marburgvirus, Ebolavirus, and Cuevavirus. Marburgvirus comprises 1 species, Marburg marburgvirus, which includes Marburg virus (MARV) and Ravn virus. Ebolavirus comprises 5 species, Zaire ebolavirus (ZEBOV), Sudan ebolavirus, Bundibugyo ebolavirus, Taï Forest ebolavirus, and Reston virus (RESTV). Cuevavirus comprises 1 species, Lloviu cuevavirus (1). Filovirus-associated diseases, especially those caused by ZEBOV and MARV, are recognized as a major threat to public health, causing high rates of death among humans and nonhuman primates.

Bats have been implicated as natural reservoirs for filoviruses (2,3) on the basis of serologic evidence from 19 bat species in 8 countries across Asia, Africa, and Europe (2,49). In addition, filovirus RNA has been detected in 8 bat species from 7 countries in the same regions (24,1013). Outbreaks of Marburg hemorrhagic fever among miners in Uganda in 2007 were traced to bat MARV (11). In addition, we previously discovered filovirus antibodies in several bat species in China (14). This finding was further confirmed by He et al., who detected filovirus RNA in brown fruit bats (Rousettus leschenaultii) in China (10). Considering the diversity of bat species in the world, long-term surveillance of bat filoviruses is essential for better understanding of distribution, diversity, and ecology of these viruses. We conducted a study to determine the diversity of filoviruses among bats in Yunnan Province, China.

The Study

Figure 1

Thumbnail of Bat collection sites for a study on genetically diverse filoviruses in Rousettus and Eonycteris spp. bats in China. Triangles indicate Jinghong City and Mengla County, Yunnan Province, where 150 apparently healthy adult bats were collected from 2 caves in November 2009 (Jinghong City) and December 2015 (Mengla County). Inset map shows the location of Yunnan Province in China.

Figure 1. Bat collection sites for a study on genetically diverse filoviruses in Rousettus and Eonycteris spp. bats in China. Triangles indicate Jinghong City and Mengla County, Yunnan Province, where 150 apparently healthy...

We captured 150 apparently healthy adult bats from 2 caves in Yunnan Province, China: 1 in Jinghong City in November 2009, and 1 in Mengla County in December 2015 (Table 1; Figure 1). The bat species we collected were Hipposideros armiger, Aselliscus stoliczkanus, Myotis ricketti, Rhinolophus Monoceros, Miniopterus fuscus, Ia io, Eonycteris spelaea, and Rousettus sp. We humanely killed all bats and collected their hearts, intestines, lungs, spleens, kidneys, livers, brains, and blood for testing. We used 2 methods to analyze bat lung tissues for presence of filovirus RNA: first, we used nested PCR with the primers FV F1/R1 and FV F2/R2 (10), and next, we used quantitative PCR (qPCR) with 3 groups of qPCR with primers and probes designed from viral sequences obtained in this study (Technical Appendix Table).

Figure 2

Thumbnail of Phylogenetic analysis of filovirus isolates collected in study of genetically diverse filoviruses in Rousettus and Eonycteris spp. bats in China, compared with reference isolates. Analysis was based on a 310-bp segment of the filovirus L gene. Bootstrap values lower than 50 are not shown. The maximum-likelihood tree was constructed based on the 310-bp segment with 1,000 bootstrap replicates. The sequences obtained in this study are marked with a triangle (group 1), black dot (group

Figure 2. Phylogenetic analysis of filovirus isolates collected in study of genetically diverse filoviruses in Rousettus and Eonycteris spp. bats in China, compared with reference isolates. Analysis was based on a 310-bp segment...

Using degenerate nested PCR, we detected filovirus RNA in 15 fruit bat specimens (E. spelaea and Rousettus sp.); the specimens comprised 10 (23.3%) of 43 E. spelaea and Rousettus sp. collected in 2009 and 5 (11.9%) of 42 collected in 2015. Using qPCR, we detected filovirus RNA in 20 specimens from E. spelaea (n = 4) and Rousettus sp. (n = 16) bats: 10 (23.3%) of the bats were collected in 2009 and 10 (23.8%) in 2015. No filovirus was detected in other bat species studied (Table 1). The 310-bp L gene sequences (GenBank accession nos. KX371873–KX371890) exhibited 65%–99% nt identity among themselves and 61%–99% nt identity with known filoviruses. Phylogenetic analysis showed that the sequences from the bats formed 3 independent groups, groups 1–3. Groups 1 and 2 comprised 6 and 11 sequences, respectively, all of which were obtained in this study (Figure 2). Group 3 comprised 2 sequences, 1 from this study and 1 previously published (10). Pairwise distance analysis indicated that sequences in group 1 share the highest nucleotide identity (75%–78%) with MARV and those in group 2 share the highest identity (69%–74%) with Ravn virus. The 2 sequences in group 3 are highly similar and share 66%–70% nt identity with other filovirus species. Of note, 1 bat specimen (no. 9447) was co-infected with 4 different filovirus strains (BtFiloYN9447–1 to 9447–4) with high divergence (Figure 2; Technical Appendix Figure 1). To further determine the phylogenetic relationship of these viruses with known filoviruses, we amplified more L gene sequence (1,475 bp) for strains BtFiloYN2162 and BtFiloYN9447–1. Similar to the 310-bp sequences, the 1,475-bp sequence of the BtfiloYN2162 shared 99% identity with BtDH04 at the nucleotide level, the 1,475-bp sequence of BtFiloYN9447–1 shared 62%–71% with known filoviruses.

To determine the tissue tropism of these viruses, we performed qPCR with primers and probes designed for each of the 3 different groups (online Technical Appendix Table). Results showed that filoviruses were mainly located in the lung and that genome copy numbers ranged from 29 to 523,582/mg of tissue (Table 2). Only 2 bat blood samples (nos. 2202 and 9447) were positive for filovirus RNA; 5 samples (nos. 2202, 2188, 9434, 9442, and 9447) contained filoviruses with more widespread tissue tropism. We were unable to isolate virus from PCR-positive samples by using Vero-E6 cells.

To detect filovirus IgG and IgM, we expressed His-tagged truncated nucleoproteins from RESTV or ZEBOV in Escherichia coli and used them as antigens (online Technical Appendix). In this experiment, we used 25 bat samples from 2015 that had enough serum volume for testing; 14 samples showed a strong cross-reaction with the ZEBOV nucleoprotein, and among them, 7 showed a weak cross-reaction with RESTV nucleoprotein. We used Western blotting to confirm these results; 11 of the 25 samples were positive for ZEBOV nucleoprotein and 4 for RESTV nucleoprotein (Table 1; Technical Appendix Figure 2). No samples overlapped between those identified as positive by PCR and those identified as positive by serologic testing. Results of a serum neutralization assay with HIV pseudovirus carrying the ZEBOV glycoprotein showed that the ELISA-positive samples had no cross-neutralization activity to the pseudovirus (14).


We detected novel filovirus sequences with high divergence in E. spelaea and Rousettus sp. bats in China. Phylogenetic analysis of partial sequences suggested that at least 3 distinct groups of filovirus are circulating in fruit bats in China. The distances between these sequences indicates that the 3 groups may represent 3 novel species or genera. Of interest, we detected antibodies reacting more strongly to ZEBOV than RESTV nucleoprotein in some filovirus RNA–negative samples, suggesting that the bats were infected with another/other filovirus(es) cross-reactive with ZEBOV nucleoprotein or that nucleoproteins of the novel filoviruses were cross-reactive with ZEBOV and RESTV nucleoproteins.

The bat samples in this study were collected from 2 caves in 2009 and 2015, respectively; the caves are ≈200 km metric apart. Across the 2 different years and locations, we detected closely related viruses and found 1 bat that was acutely co-infected by 4 different filoviruses; this finding suggests that these viruses have been circulating in the 2 bat species and that densely populated bat caves provide opportunity for cross-infection with different viruses. However, considering the migration ability of the fruit bat, we cannot exclude the possibility that there are exchanges of virus between the bats in these two caves. Longitudinal surveillance with tracking tags may help to better understand the spatial–temporal distribution of these viruses in bat populations.

In previous reports, filoviruses were primarily detected in liver and spleen tissues (4,15). In our study, we primarily detected filoviruses in the lung. We suspect that lung tissues are the major target for these bat filoviruses. Thus, these filoviruses may have the potential to be transmitted through the respiratory tract.

These results will be helpful in providing a better understanding of the distribution and diversity of filoviruses, which may have implications for public health. Considering their feeding habitats, fruit bats are often in close contact with domestic animals and human populations. It is therefore necessary to establish long-term and proactive surveillance of these viruses and related diseases.

Dr. Yang is as a research assistant at Wuhan Institute of Virology, Chinese Academy of Sciences. His primary research interests include viral epidemiology and viral characterization of small mammals.



This work was funded by a joint National Natural Science Foundation of China grant (81290341) and the China Mega-Project for Infectious Disease grant (2014ZX10004001-003) from the Minister of Science and Technology of the People’s Republic of China (to Z-L.S.); a Scientific and Technological Basis Special Project grant (2013FY113500; to Z-L.S. and Y-Z.Z.); a National Natural Science Foundation of China grant (81260437) and State Key Laboratory for Infectious Diseases Prevention and Control grant (2013SKLID302) (to Y-Z.Z.); and a United States Agency for International Development Emerging Pandemic Threats PREDICT project grant (cooperative agreement AID-OAA-A-14-00102; to P.D.D.).



  1. King  AMQ, Adams  MJ, Carstens  EB, Lefkowitz  EJ, editors. Virus taxonomy: ninth report of the International Committee on Taxonomy of Viruses. San Diego (CA): Academic Press; 2012.
  2. Olival  KJ, Hayman  DT. Filoviruses in bats: current knowledge and future directions. Viruses. 2014;6:175988. DOIPubMed
  3. Jayme  SI, Field  HE, de Jong  C, Olival  KJ, Marsh  G, Tagtag  AM, et al. Molecular evidence of Ebola Reston virus infection in Philippine bats. Virol J. 2015;12:107. DOIPubMed
  4. Leroy  EM, Kumulungui  B, Pourrut  X, Rouquet  P, Hassanin  A, Yaba  P, et al. Fruit bats as reservoirs of Ebola virus. Nature. 2005;438:5756. DOIPubMed
  5. Swanepoel  R, Smit  SB, Rollin  PE, Formenty  P, Leman  PA, Kemp  A, et al.; International Scientific and Technical Committee for Marburg Hemorrhagic Fever Control in the Democratic Republic of Congo. Studies of reservoir hosts for Marburg virus. Emerg Infect Dis. 2007;13:184751. DOIPubMed
  6. Kuzmin  IV, Niezgoda  M, Franka  R, Agwanda  B, Markotter  W, Breiman  RF, et al. Marburg virus in fruit bat, Kenya. Emerg Infect Dis. 2010;16:3524. DOIPubMed
  7. Taniguchi  S, Watanabe  S, Masangkay  JS, Omatsu  T, Ikegami  T, Alviola  P, et al. Reston Ebolavirus antibodies in bats, the Philippines. Emerg Infect Dis. 2011;17:155960.PubMed
  8. Olival  KJ, Islam  A, Yu  M, Anthony  SJ, Epstein  JH, Khan  SA, et al. Ebola virus antibodies in fruit bats, bangladesh. Emerg Infect Dis. 2013;19:2703. DOIPubMed
  9. Towner  JS, Pourrut  X, Albariño  CG, Nkogue  CN, Bird  BH, Grard  G, et al. Marburg virus infection detected in a common African bat. PLoS One. 2007;2:e764. DOIPubMed
  10. He  B, Feng  Y, Zhang  H, Xu  L, Yang  W, Zhang  Y, et al. Filovirus RNA in Fruit Bats, China. Emerg Infect Dis. 2015;21:16757. DOIPubMed
  11. Towner  JS, Amman  BR, Sealy  TK, Carroll  SA, Comer  JA, Kemp  A, et al. Isolation of genetically diverse Marburg viruses from Egyptian fruit bats. PLoS Pathog. 2009;5:e1000536. DOIPubMed
  12. Negredo  A, Palacios  G, Vázquez-Morón  S, González  F, Dopazo  H, Molero  F, et al. Discovery of an ebolavirus-like filovirus in europe. PLoS Pathog. 2011;7:e1002304. DOIPubMed
  13. Amman  BR, Carroll  SA, Reed  ZD, Sealy  TK, Balinandi  S, Swanepoel  R, et al. Seasonal pulses of Marburg virus circulation in juvenile Rousettus aegyptiacus bats coincide with periods of increased risk of human infection. PLoS Pathog. 2012;8:e1002877. DOIPubMed
  14. Yuan  J, Zhang  Y, Li  J, Zhang  Y, Wang  LF, Shi  Z. Serological evidence of ebolavirus infection in bats, China. Virol J. 2012;9:236. DOIPubMed
  15. Brauburger  K, Hume  AJ, Mühlberger  E, Olejnik  J. Forty-five years of Marburg virus research. Viruses. 2012;4:1878927. DOIPubMed





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DOI: 10.3201/eid2303.161119

1These authors contributed equally to this article.

Table of Contents – Volume 23, Number 3—March 2017


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Zheng-Li Shi, Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

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