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Volume 23, Number 3—March 2017
Research Letter

Molecular Verification of New World Mansonella perstans Parasitemias

Lucyane Bastos Tavares da Silva1, James Lee Crainey1Comments to Author , Túllio Romão Ribeiro da Silva, Uziel Ferreira Suwa, Ana Carolina Paulo Vicente, Jansen Fernandes de Medeiros, Felipe Arley Costa Pessoa, and Sérgio Luiz Bessa Luz
Author affiliations: Fundação Oswaldo Cruz Amazônia Instituto Leônidas e Maria Deane, Amazonas, Brazil (L.B. Tavares da Silva, J.L. Crainey, T.R. Ribeiro da Silva, U.F. Suwa, F.A.C. Pessoa, S.L. Bessa Luz); Fundação Oswaldo Cruz Instituto Oswaldo Cruz, Rio de Janeiro, Brazil (A.C. Paulo Vicente); Fundação Oswaldo Cruz Rondônia Laboratório de Entomologia, Rondônia, Brazil (J. Fernandes de Medeiros)

Main Article

Figure

Maximum-likelihood phylogenetic trees showing the relationship between Mansonella parasites from Amazon region of Brazil (Amazonas state) and some of their closest relatives. A) Ribosomal internal transcribed spacer 1–based phylogeny. B) Mitochondrial cytochrome c oxidase subunit 1–based phylogeny. C) Mitochondrial 12S-based phylogeny. All 3 trees were prepared by using DNA sequence alignments and PHYLIP version 3.67 (http://evolution.genetics.washington.edu/phylip.html). Black circles indicate

Figure. Maximum-likelihood phylogenetic trees showing the relationship between Mansonella parasites from Amazon region of Brazil (Amazonas state) and some of their closest relatives. A) Ribosomal internal transcribed spacer 1–based phylogeny. B) Mitochondrial cytochrome c oxidase subunit 1–based phylogeny. C) Mitochondrial 12S-based phylogeny. All 3 trees were prepared by using DNA sequence alignments and PHYLIP version 3.67 (http://evolution.genetics.washington.edu/phylip.html). Black circles indicate significant bootstrap-supported nodes as a percentage of 1,000 pseudoreplicates. Solid boxes indicate M. perstans and dashed boxes M. ozzardi sequences generated for this study and used in the construction of the displayed trees. Scale bars indicate nucleotide substitutions per site. These sequence have been submitted to GenBank and EMBL (accession nos.: M. perstans cytochrome c oxidase subunit 1, LT623909; M. ozzardi cytochrome c oxidase subunit 1, LT623910; M. perstans 12S, LT623913; M. ozzardi 12S, LT623914; M. perstans internal transcribed spacer 1, LT623911; and M. ozzardi internal transcribed spacer 1, LT623912).

Main Article

1These authors contributed equally to this article.

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