Volume 23, Number 3—March 2017
Research Letter
Serologic Evidence for MERS-CoV Infection in Dromedary Camels, Punjab, Pakistan, 2012–2015
Table
Detection of Middle East respiratory syndrome coronavirus antibodies in dromedary camels from different districts of Punjab Province, Pakistan, 2012–2015*
District |
Year of sampling† |
No. animals tested |
ELISA | MN | |||
---|---|---|---|---|---|---|---|
No. positive |
Detection rate, % (95% CI) |
No. positive |
Detection rate, % (95% CI) |
||||
Chiniot | 2012, 2014 | 23 | 4 | 17.4 (5–38.8) | 0 | 0 (0.0–14.8) | |
Faisalabad | 2012, 2013, 2015 | 66 | 45 | 68.2 (55.6–79.1) | 32 | 48.5 (36.0–61.1) | |
Jhang | 2012, 2013, 2015 | 220 | 79 | 35.9 (29.6–42.6) | 53 | 24.1 (18.6–30.3) | |
Bhakkar | 2012 | 88 | 51 | 57.9 (47–68.4) | 36 | 40.9 (30.5–51.9) | |
Layyah | 2012 | 13 | 9 | 69.2 (38.6–90.9) | 4 | 30.8 (9.1–61.4) | |
Muzaffargarh | 2012, 2013 | 40 | 26 | 65 (48.3–79.4) | 15 | 37.5 (22.7–54.2) | |
Bahawalpur | 2015 | 29 | 26 | 89.7 (72.6–97.8) | 21 | 72.4 (52.8–87.3) | |
Lodhran | 2014 | 51 | 42 | 82.4 (69.1–91.6) | 33 | 64.7 (50.1–77.6) | |
Rahim Yar Khan |
2012 |
35 |
33 |
94.3 (80.8–99.3) |
29 |
82.9 (66.4–93.4) |
|
Total | 565 | 315 | 55.8 (51.5–59.9) | 223 | 39.5 (35.4–43.6) |
*Serum samples were tested at a dilution of 1:100; MNT was done in a microtiter plate format in duplicate at a dilution of 1:80. ELISA ratio >0.4 and MN >1:80 were considered positive. MN, microneutralization.
†Samples from all years, except the samples from 2012 and 2014 from Chiniot, were MN positive.
1These authors contributed equally to this article.