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Volume 23, Number 3—March 2017
Research Letter

Serologic Evidence for MERS-CoV Infection in Dromedary Camels, Punjab, Pakistan, 2012–2015

Muhammad Saqib1, Andrea Sieberg1, Muhammad Hammad Hussain, Muhammad Khalid Mansoor, Ali Zohaib, Erik Lattwein, Marcel Alexander Müller, Christian DrostenComments to Author , and Victor Max Corman
Author affiliations: Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan (M. Saqib, M.H. Hussain); University of Bonn Medical Centre, Bonn, Germany (A. Sieberg, M.A. Müller, C. Drosten, V.M. Corman); Animal Health Research Center, Ministry of Agriculture and Fisheries, Muscat, Oman (M.H. Hussain, M.K. Mansoor); Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China (A. Zohaib); EUROIMMUN AG, Lübeck, Germany (E. Lattwein); German Centre for Infection Research, Bonn (C. Drosten, V.M. Corman)

Main Article

Table

Detection of Middle East respiratory syndrome coronavirus antibodies in dromedary camels from different districts of Punjab Province, Pakistan, 2012–2015*

District
 Year of sampling†
 No. animals tested
 ELISA
MN
No. positive
 Detection rate, % (95% CI)
 No. positive
 Detection rate, % (95% CI)
Chiniot 2012, 2014 23 4 17.4 (5–38.8) 0 0 (0.0–14.8)
Faisalabad 2012, 2013, 2015 66 45 68.2 (55.6–79.1) 32 48.5 (36.0–61.1)
Jhang 2012, 2013, 2015 220 79 35.9 (29.6–42.6) 53 24.1 (18.6–30.3)
Bhakkar 2012 88 51 57.9 (47–68.4) 36 40.9 (30.5–51.9)
Layyah 2012 13 9 69.2 (38.6–90.9) 4 30.8 (9.1–61.4)
Muzaffargarh 2012, 2013 40 26 65 (48.3–79.4) 15 37.5 (22.7–54.2)
Bahawalpur 2015 29 26 89.7 (72.6–97.8) 21 72.4 (52.8–87.3)
Lodhran 2014 51 42 82.4 (69.1–91.6) 33 64.7 (50.1–77.6)
Rahim Yar Khan
 2012
 35
 33
 94.3 (80.8–99.3)
29
 82.9 (66.4–93.4)
Total 565 315 55.8 (51.5–59.9) 223 39.5 (35.4–43.6)

*Serum samples were tested at a dilution of 1:100; MNT was done in a microtiter plate format in duplicate at a dilution of 1:80. ELISA ratio >0.4 and MN >1:80 were considered positive. MN, microneutralization.
†Samples from all years, except the samples from 2012 and 2014 from Chiniot, were MN positive.

Main Article

1These authors contributed equally to this article.

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