Volume 23, Number 4—April 2017
Dispatch
Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay
Kristen L. Burkhalter
and Harry M. Savage
and Harry M. Savage
Table
Zika virus titers for infected mosquitoes stored at room temperature (21°C) and collected every other day for 2–30 days and control samples*
| Day of collection | No. samples with live virus/no. tested (%) | Mean titer, log10 PFU/mL (95% CI) |
|---|---|---|
| 0 | 9/9 (100.0) | 6.62 (6.52–6.72) |
| 2 | 6/6 (100.0) | 2.3 (2.06–2.54) |
| 4 | 2/6 (33.3) | 1.85 (0.63–3.07) |
| 6 | 1/6 (16.6) | 1.69 |
| 8 | 0/6 (0) | NA |
| 10 | 1/6 (16.6) | 2.5 |
| 12–30 | 0/6 per day (0) | NA |
*Control samples (day 0) were stored at −80°C until testing. NA, not applicable.
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