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Volume 23, Number 4—April 2017

Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay

Kristen L. BurkhalterComments to Author  and Harry M. Savage
Author affiliations: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA

Main Article


Zika virus titers for infected mosquitoes stored at room temperature (21°C) and collected every other day for 2–30 days and control samples*

Day of collection No. samples with live virus/no. tested (%) Mean titer, log10 PFU/mL (95% CI)
0 9/9 (100.0) 6.62 (6.52–6.72)
2 6/6 (100.0) 2.3 (2.06–2.54)
4 2/6 (33.3) 1.85 (0.63–3.07)
6 1/6 (16.6) 1.69
8 0/6 (0) NA
10 1/6 (16.6) 2.5
12–30 0/6 per day (0) NA

*Control samples (day 0) were stored at −80°C until testing. NA, not applicable.

Main Article

Page created: March 17, 2017
Page updated: March 17, 2017
Page reviewed: March 17, 2017
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.