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Volume 23, Number 4—April 2017

Dispatch

Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay

Kristen L. BurkhalterComments to Author  and Harry M. Savage
Author affiliations: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA

Main Article

Table

Zika virus titers for infected mosquitoes stored at room temperature (21°C) and collected every other day for 2–30 days and control samples*

Day of collection No. samples with live virus/no. tested (%) Mean titer, log10 PFU/mL (95% CI)
0 9/9 (100.0) 6.62 (6.52–6.72)
2 6/6 (100.0) 2.3 (2.06–2.54)
4 2/6 (33.3) 1.85 (0.63–3.07)
6 1/6 (16.6) 1.69
8 0/6 (0) NA
10 1/6 (16.6) 2.5
12–30 0/6 per day (0) NA

*Control samples (day 0) were stored at −80°C until testing. NA, not applicable.

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