Figure 2. Phylogenetic trees for norovirus GII.2 strains, Taiwan, 2014–2016. A) Partial ORF1 nucleotide sequences in RdRp region (644 nt) of GII.2 strains aligned and the tree generated by using Kimura 80 substitution model with gamma site rates, 1,000 bootstrap replicates, by using MEGA 6.0 software (http://www.megasoftware.net). Bootstrap values of 1,000 replications are shown on the branches. B) Full-length ORF2 nucleotide sequences of GII.2 strains aligned and the tree generated by using Tamura-Nei substitution model with gamma site rates with invariant site using MEGA 6.0 software with 1,000 bootstrap replicates. Bootstrap values of 1,000 replications are shown on the branches. For both trees, norovirus GII.2 reference sequences were downloaded from GenBank; accession number, country, and year are shown. Sequences from Taiwan are indicated by outbreak number and collection year and month. Taiwan GII.2 strains for 2016: 25-1 201610, 14-18 201611, 7-11 201611, 12-7 201610, 24-11 201610, 21-2 201610, 14-33 201611, 11-2 201611, and 18-5 201611; for 2015: 17-10 201503 and 12-17 201503; for 2014: 20-1 20141, 23-8 201412, and 4-1 201411. Corresponding GenBank accession numbers are KY457721–KY457736. BGD, Bangladesh; DE, Germany; HK, Hong Kong; JP, Japan; KR, South Korea; ORF, open reading frame; RdRp, RNA-dependent RNA polymerase; RUS, Russia; TW, Taiwan; US, United States; VP, viral protein. Scale bars indicate nucleotide substitutions per site.