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Volume 24, Number 1—January 2018
Dispatch

Mammalian Pathogenesis and Transmission of Avian Influenza A(H7N9) Viruses, Tennessee, USA, 2017

Jessica A. Belser, Nicole Brock, Xiangjie Sun, Joyce Jones, Natosha Zanders, Erin Hodges, Joanna A. Pulit-Penaloza, David Wentworth, Terrence M. Tumpey, Todd Davis, and Taronna R. MainesComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA

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Figure 2

Replication kinetics of avian influenza A(H7N9) influenza viruses in human respiratory tract cells, Tennessee, USA, 2017, compared with strains from Nebraska (gs/NE) and Asia (Anhui/1). Calu-3 cells (American Type Culture Collection, Manassas, VA, USA) were grown to confluence in 12-mm diameter transwell inserts (Corning, Corning, NY, USA), infected apically with viruses shown at a multiplicity of infection of 0.01 (A and C) or 0.001 (B) 1 h, washed, and incubated at 37°C (A and B) or 33°C (C).

Figure 2. Replication kinetics of avian influenza A(H7N9) viruses in human respiratory tract cells, Tennessee, USA, 2017, compared with strains from Nebraska (gs/NE) and Asia (Anhui/1). Calu-3 cells (American Type Culture Collection, Manassas, VA, USA) were grown to confluence in 12-mm–diameter transwell inserts (Corning, Corning, NY, USA), infected apically with viruses shown at a multiplicity of infection of 0.01 (A and C) or 0.001 (B) 1 h, washed, and incubated at 37°C (A and B) or 33°C (C). Supernatants were removed at indicated times postinoculation, and titers of infectious virus were determined by titration in eggs. The limit of virus detection was 101.5 EID50/mL. Values are mean from triplicate independent cultures per virus. Error bars indicate SDs. *p<0.05 for HPAI vs. LPAI ck/TN viruses by 2-way analysis of variance with a Tukey posttest. EID50, 50% egg infectious dose; gs, goose; HPAI, highly pathogenic avian influenza virus; LPAI, low pathogenicity avian influenza virus.

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