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Volume 24, Number 10—October 2018
Dispatch

Mycobacterium caprae Infection in Captive Borneo Elephant, Japan

Shiomi YoshidaComments to Author , Satomi Suga, Satoshi Ishikawa, Yasuhiko Mukai, Kazunari Tsuyuguchi, Yoshikazu Inoue, Taro Yamamoto, and Takayuki Wada
Author affiliations: Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan (S. Yoshida); National Hospital Organization Kinki-chuo Chest Medical Center Clinical Research Center, Sakai, Japan (S. Yoshida, K. Tsuyuguchi, Y. Inoue); Fukuyama Zoo, Fukuyama, Japan (S. Suga, S. Ishikawa, Y. Mukai); Nagasaki University Institute of Tropical Medicine, Nagasaki (T. Yamamoto, T. Wada); Nagasaki University School of Tropical Medicine and Global Health, Nagasaki (T. Wada)

Main Article

Figure

Phylogenetic tree of isolate EPDC01 from a captive Borneo elephant with Mycobacterium caprae infection, Japan, 2016, and 8 Mycobacterium caprae strains (Allgäu and Lechtal types) from a report by Broeckl et al. (13). Short reads of M. caprae strains were assembled by CLC Genomics Workbench version 9.5.1 (https://www.qiagenbioinformatics.com/solutions/functional-genomics/?gclid=EAIaIQobChMIvvGL3L7T2wIVTSOBCh2FAAKtEAAYASAAEgKLWvD_BwE) before analysis. Core single-nucleotide polymorphisms of all 13

Figure. Phylogenetic tree of isolate EPDC01 from a captive Borneo elephant with Mycobacterium caprae infection, Japan, 2016, and 8 Mycobacterium caprae strains (Allgäu and Lechtal types) from a report by Broeckl et al. (13). Short reads of M. caprae strains were assembled by CLC Genomics Workbench version 9.5.1 (https://www.qiagenbioinformatics.com/solutions/functional-genomics/?gclid=EAIaIQobChMIvvGL3L7T2wIVTSOBCh2FAAKtEAAYASAAEgKLWvD_BwE) before analysis. Core single-nucleotide polymorphisms of all 13 strains, including reference M. tuberculosis complex strains (M. bovis, AF2122/97 [GenBank accession no. NC_002945.4]: M. orygis, 112400015 [NZ_APKD00000000.1]: M. pinnipedii, BAA-688 [MWXB00000000.1]: M. microti, strain 12 [CP010333.1]: and M. mungi, BM22813 [NZ_LXTB00000000.1]), were determined and used for tree construction based on neighbor-joining by kSNP3 (12). A tree including all 61 strains described by Broeckl et al. (13) is shown in the Technical Appendix

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