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Volume 24, Number 11—November 2018
Dispatch

Fatal Case of Diphtheria and Risk for Reemergence, Singapore

Yingqi LaiComments to Author , Parthasarathy Purnima, Marc Ho, Michelle Ang, Rama N. Deepak, Ka Lip Chew, Shawn Vasoo, Dimatatac F. Capulong, and Vernon Lee
Author affiliations: Ministry of Health, Singapore (Y. Lai, M. Ho, M. Ang, V. Lee); Khoo Teck Puat Hospital, Singapore (P. Purnima, R.N. Deepak); National Public Health Laboratory, Singapore (M. Ang); National University Hospital, Singapore (K.L. Chew); Tan Tock Seng Hospital, Singapore (S. Vasoo, D.F. Capulong)

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Figure 1

Phylogenetic analysis of Corynebacterium diphtheriae isolate from a 23-year-old man who died from diphtheria (OTH-17-20; bold) and 9 other isolates collected from hospitals in Singapore during 2013–2017. The tree was constructed by using 7 concatenated housekeeping gene sequences corresponding to the C. diphtheriae multilocus sequence typing scheme (https://pubmlst.org/cdiphtheriae/). Sequences were extracted from whole-genome sequences of each isolate. Concatenated sequences were aligned by usi

Figure 1. Phylogenetic analysis of Corynebacterium diphtheriae isolate from a 23-year-old man who died from diphtheria (OTH-17-20; bold) and 9 other isolates collected from hospitals in Singapore during 2013–2017. The tree was constructed by using 7 concatenated housekeeping gene sequences corresponding to the C. diphtheriae multilocus sequence typing scheme (https://pubmlst.org/cdiphtheriae/). Sequences were extracted from whole-genome sequences of each isolate. Concatenated sequences were aligned by using ClustalW (http://www.clustal.org/). Phylogeny was inferred by using the maximum-likelihood method, neighbor-joining algorithm based on the Jukes-Cantor model, and MEGA7 software (10). There were 2,544 positions in the final dataset. Numbers next to branches show bootstrap values calculated by using 1,000 reiterations. Scale bar indicates nucleotide substitutions per site. ST, sequence type.

Main Article

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