Volume 24, Number 11—November 2018
Research Letter
No Plasmodium falciparum Chloroquine Resistance Transporter and Artemisinin Resistance Mutations, Haiti
Jeanne P. Vincent, Kanako Komaki-Yasuda, Alexandre V. Existe, Jacques Boncy, and Shigeyuki Kano
Table
Primers used for nested PCRs to detect Plasmodium falciparum chloroquine resistance transporter and artemisin gene resistance mutations, Haiti*
| Target | Primer sequences, 5′→3′ | Primer annealing positions |
|---|---|---|
| pfcrt, primary PCR |
F: ATGGCTCACGTTTAGGTGGAGGT |
92–114 |
| R: CGGATGTTACAAAACTATAGTTACCA |
258–283 |
|
| pfcrt, secondary PCR |
F: GTCTTGGTAAATGTGCTCATGTGT |
119–142 |
| R: CTATAGTTACCAATTTTGTTTAAAGTTCT |
241–269 |
|
| k13, primary PCR |
F: GAAGCCTTGTTGAAAGAAGCA |
1276–1296 |
| R: CCAAGCTGCCATTCATTTGT |
2107–2126 |
|
| k13, secondary PCR | F: GCCTTGTTGAAAGAAGCAGAA |
1279–1299 |
| R: GTGGCAGCTCCAAAATTCAT | 2011–2030 |
*Secondary PCR products were directly sequenced by using the BigDye Terminator version 3.1 Cycle Sequencing Kit and analyzed with a 3130xl Genetic Analyzer (both from Thermo Fisher Scientific Inc., Waltham, MA, USA). F, forward; k13, Kelch 13; pfcrt, P. falciparum chloroquine resistance transporter; R, reverse.
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Page updated: October 17, 2018
Page reviewed: October 17, 2018
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