Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 24, Number 12—December 2018

CME ACTIVITY - Synopsis

Reemergence of St. Louis Encephalitis Virus in the Americas

Adrián DiazComments to Author , Lark L. Coffey, Nathan Burkett-Cadena, and Jonathan F. Day
Author affiliations: Universidad Nacional de Córdoba, Córdoba, Argentina (A. Diaz); University of California, Davis, California, USA (L.L. Coffey); University of Florida, Vero Beach, Florida, USA (N. Burkett-Cadena, J.F. Day)

Cite This Article

Close

Highlight and copy the desired format.

EID Diaz A, Coffey LL, Burkett-Cadena N, Day JF. Reemergence of St. Louis Encephalitis Virus in the Americas. Emerg Infect Dis. 2018;24(12):2150-2157. https://dx.doi.org/10.3201/eid2412.180372
AMA Diaz A, Coffey LL, Burkett-Cadena N, et al. Reemergence of St. Louis Encephalitis Virus in the Americas. Emerging Infectious Diseases. 2018;24(12):2150-2157. doi:10.3201/eid2412.180372.
APA Diaz, A., Coffey, L. L., Burkett-Cadena, N., & Day, J. F. (2018). Reemergence of St. Louis Encephalitis Virus in the Americas. Emerging Infectious Diseases, 24(12), 2150-2157. https://dx.doi.org/10.3201/eid2412.180372.

Introduction

CME Logo

In support of improving patient care, this activity has been planned and implemented by Medscape, LLC and Emerging Infectious Diseases. Medscape, LLC is jointly accredited by the Accreditation Council for Continuing Medical Education (ACCME), the Accreditation Council for Pharmacy Education (ACPE), and the American Nurses Credentialing Center (ANCC), to provide continuing education for the healthcare team.

Medscape, LLC designates this Journal-based CME activity for a maximum of 1.00 AMA PRA Category 1 Credit(s)™. Physicians should claim only the credit commensurate with the extent of their participation in the activity.

All other clinicians completing this activity will be issued a certificate of participation. To participate in this journal CME activity: (1) review the learning objectives and author disclosures; (2) study the education content; (3) take the post-test with a 75% minimum passing score and complete the evaluation at http://www.medscape.org/journal/eid; and (4) view/print certificate.

Release date: November 14, 2018; Expiration date: November 14, 2019

Learning Objectives

Upon completion of this activity, participants will be able to:

• Examine the history of St. Louis encephalitis virus epidemiology in the United States

• Evaluate the emergence and reemergence of St. Louis encephalitis virus in South America

• Assess the reemergence of St. Louis encephalitis virus in the western United States

CME Editor

Deborah Wenger, MBA, Copyeditor, Emerging Infectious Diseases. Disclosure: Deborah Wenger, MBA, has disclosed no relevant financial relationships.

CME Author

Laurie Barclay, MD, freelance writer and reviewer, Medscape, LLC. Disclosure: Laurie Barclay, MD, has disclosed the following relevant financial relationships: owns stock, stock options, or bonds from Pfizer.

Authors

Disclosures: Adrián Diaz, PhD; Lark L. Coffey, PhD; Nathan Burkett-Cadena, PhD; and Jonathan F. Day, PhD, have disclosed no relevant financial relationships.

Top

Abstract

We summarize and analyze historical and current data regarding the reemergence of St. Louis encephalitis virus (SLEV; genus Flavivirus) in the Americas. Historically, SLEV caused encephalitis outbreaks in the United States; however, it was not considered a public health concern in the rest of the Americas. After the introduction of West Nile virus in 1999, activity of SLEV decreased considerably in the United States. During 2014–2015, SLEV caused a human outbreak in Arizona and caused isolated human cases in California in 2016 and 2017. Phylogenetic analyses indicate that the emerging SLEV in the western United States is related to the epidemic strains isolated during a human encephalitis outbreak in Córdoba, Argentina, in 2005. Ecoepidemiologic studies suggest that the emergence of SLEV in Argentina was caused by the introduction of a more pathogenic strain and increasing populations of the eared dove (amplifying host).

The disease known as St. Louis encephalitis (SLE) is caused by St. Louis encephalitis virus (SLEV), identified as the causative agent of a mosquitoborne viral epidemic in St. Louis, Missouri, USA, during the summer of 1933 (1). SLEV is transmitted by numerous mosquito species in the genus Culex and is amplified by passerine and columbiform avian species (1). Phylogenetic analysis based on the full-length E gene sequences grouped SLEV strains into 8 genotypes (2). Genotypes I and II are prevalent in the United States and genotype V is widely distributed in South America. Other genotypes have limited distribution: genotype III is in southern South America, IV is limited to Colombia and Panama, VI is in Panama, VII is in Argentina, and VIII has been detected only in the Amazon region of Brazil (2).

A retrospective analysis revealed that 38 human cases and 14 deaths were caused by SLEV in Paris, Illinois, USA during the summer of 1932 (3). A 1933 SLE epidemic resulted in 1,095 clinical human cases and 201 deaths (3). Because subclinical cases are not identified or reported, retrospective serosurveys were conducted to determine the ratio of subclinical to clinical infections, which was determined to be 300:1 (3). Using this ratio, the actual number of SLE cases during the 1933 SLE epidemic was ≈328,500, affecting nearly 40% of the city’s 821,960 inhabitants, based on US census data for 1930 (4).

Figure 1

Thumbnail of Geographic distribution of historical St. Louis encephalitis human cases reported in the Americas through November 2017. Dot size represents the number of human cases reported in each episode. Colors represent year of detection.

Figure 1. Geographic distribution of historical St. Louis encephalitis human cases reported in the Americas through November 2017. Dot size represents the number of human cases reported in each episode. Colors represent year...

Since SLEV was first identified, 4 human SLE transmission scenarios have been reported (5). First, during most years, no human SLE infections are reported. However, SLEV transmission to sentinel animals and virus isolation from mosquito pools is documented in the absence of human cases. Second, small numbers of spatially and temporally isolated human SLE cases occur. For example, in 1993, 8 human SLE cases were reported in Lee and Collier counties, Florida, USA; 5 of the cases reported onset during October (6). Third, sporadic transmission occurs as widely dispersed (temporarily and spatially) individual human cases. For example, in 1997, 9 human SLE cases were reported from 6 Florida counties, ranging from Brevard County on the central Atlantic Coast south to Lee County on the southern Gulf Coast. Onset for these cases ranged from July through late October (6). Finally, epidemic transmission occurs as focused (in space and time) clusters of human clinical cases: for example, an extensive 1975 epidemic that occurred along the Mississippi and Ohio River basins from Ontario, Canada; Cleveland, Ohio; and Chicago, Illinois, in the north to Birmingham, Alabama, and Mississippi in the south. Well-documented SLE outbreaks include the 1933 St. Louis epidemic and the 1959, 1961, 1962, 1977, and 1990 epidemics in south Florida. Other epidemics of note occurred in St. Louis (1937); Hidalgo County, Texas (1954); High Plains, Texas; Louisville, Kentucky; and Cameron County, Texas (1956); and Houston, Texas (1964). There was also a 1990 SLE epidemic in Florida (5), and, most recently, a 2015 epidemic in the Phoenix area of Arizona (Figure 1).

Epidemics of SLE are promoted by environmental factors including summer temperature, rainfall, snowmelt, and surface water conditions (7,8). One of the most notable environmental drivers for SLEV activity is the cycling of rainfall and drought. The wet–dry cycle can affect the epidemiology of SLEV by forcing gravid floodwater Culex vectors to delay oviposition long enough to complete viral development (extrinsic incubation) in a single gonotrophic cycle, thus making them capable of viral transmission during their second blood meal (8). Drought has also been linked to urban SLE and West Nile virus (WNV) epidemics involving vectors in the Cx. pipiens complex (9).

Transmission Cycles

The 4 primary vectors of SLEV in the United States are Cx. pipiens pipiens Linnaeus, Cx. pipiens quinquefasciatus Say, Cx. tarsalis Coquillett, and Cx. nigripalpus Theobald mosquitoes (1). Vector species distribution determines the geographic distribution of SLEV, and affects whether epidemics are urban or rural. Urban SLE epidemics usually involve Cx. pipiens pipiens and Cx. pipiens quinquefasciatus (Cx. pipiens complex) mosquitoes, species that oviposit in the permanent aquatic habitats provided by storm drains, sewage treatment facilities, and wastewater retention ponds. Rural SLE epidemics usually involve floodwater species, such as Cx. tarsalis and Cx. nigripalpus mosquitoes (1). Urban and rural epidemic transmission patterns are best demonstrated by the history of SLEV in Florida, where urban human SLEV cases were detected in Miami in 1952 and 1954, followed by St. Petersburg in 1959, Tampa in 1961, and Sarasota in 1962. In 1977, SLEV epidemic transmission in Florida shifted from urban to rural areas (7). The 1977 and 1990 Florida SLE epidemics both started in Indian River County and then spread throughout the Florida peninsula (7). The temporal shift from urban to rural epidemic transmission in Florida was facilitated by changes in Cx. nigripalpus oviposition preference and behavior. The Cx. nigripalpus mosquito is a widespread subtropical species and a highly opportunistic blood feeder that oviposits in freshly flooded temporary aquatic habitats. In urban habitats, Cx. nigripalpus mosquitoes oviposit in wastewater retention ponds and open wastewater outflow ditches (10). The shift to rural transmission in 1977 and 1990 was facilitated by an increase in citrus farming, as citrus groves were designed to be maintained by flood irrigation (10). In the 1970s through the 1990s, rural citrus grove drainage furrows became the preferred oviposition site for Cx. nigripalpus mosquitoes (11). The proclivity of Cx. nigripalpus mosquitoes to blood-feed on birds as well as mammals and the tendency of females to delay oviposition until the proper aquatic oviposition habitats are created by heavy summer rainfalls make it an excellent vector of SLEV (11).

The introduction of WNV into the United States in 1999 promoted debate about how the presence of WNV would affect the continued transmission of SLEV, given the serologic cross-reactivity of the 2 viruses in avian hosts. Laboratory studies demonstrated that house finches (Haemorhous mexicanus) experimentally infected with WNV developed neutralizing immunity that prevented infection after rechallenge with WNV or SLEV (12). In contrast, house finches first exposed to SLEV showed lower subsequent viremias after rechallenge with WNV. This suggests that WNV could competitively exclude SLEV from amplification in shared avian hosts like house finches (13). Indeed, transmission of SLEV in Florida decreased notably following the introduction and establishment of WNV (13). For competitive exclusion to suppress SLEV transmission, considerable host overlap between SLEV and WNV must occur. In Florida, avian species in the families Cardinalidae (northern cardinal, Cardinalis cardinalis), Columbidae (mourning dove, Zenaida macroura), Corvidae (blue jay, Cyanocitta cristata) and Icteridae (common grackle, Quiscalus quiscula) are frequently exposed to SLEV (14). For WNV, house sparrows (Passer domesticus) and blue jays are highly infectious to mosquitoes; in contrast, mourning doves were found to be 1 of the least competent WNV hosts of 25 bird species examined experimentally (15). Even if mourning doves are poor WNV hosts, being exposed to WNV may provide cross neutralization to SLEV and preempt amplification of SLEV, provided that doves are exposed to WNV early in the transmission season. In California, sparrows, finches, and corvids (house finch, house sparrow, purple finch [Haemorhous purpureus]); song sparrow (Melospiza melodia); western scrub jay (Aphelocoma californica); and white-crowned sparrow (Zonotrichia leucophrys) are considered major amplifying hosts of WNV and SLEV (1,16). As in Florida, host overlap of WNV and SLEV likely contributed to the initial disappearance of SLEV from the western United States (17). Of these avian hosts, only house sparrows, house finches, American crows (Corvus brachyrhynchos), and western scrub jays were found to be amplifying hosts capable of developing viremias above the threshold for infection of Cx. tarsalis, the principal vector of WNV and SLEV throughout much of California (16). Further work is needed to characterize the specific avian hosts fed on by Cx. pipiens quinquefasciatus and Cx. tarsalis mosquitoes in epidemic and nonepidemic settings to determine whether additional host species may be involved in amplification of SLEV and to what degree host overlap contributes to the competitive exclusion or reemergence of SLEV in areas of the United States outside California.

Since the introduction of WNV into the United States, human SLE cases continue to occur throughout the country. During 2004–2013, 92 clinical SLE cases were reported (18). Most cases were located in the Gulf Coast states of Louisiana (10 cases), Mississippi (13), and Texas (16). During 2014–2016, a total of 32 human SLE cases were reported (19). Most of these cases were reported in the Phoenix area, where a SLE epidemic resulted in 23 confirmed human cases, including 1 fatality (19). SLEV has remained endemic throughout much of the United States despite the introduction and establishment of WNV.

Emergence and Reemergence in South America

Historically, SLEV has not been considered a major public health threat in the Americas, other than in the United States. Human SLE cases have been reported sporadically throughout Latin America, but no human epidemics were reported until 2005. In Argentina, Charosky et al. (20) reported a neurologic human SLEV case in 1968. Two years later, Mettler et al. (21) isolated SLEV from a febrile human suspected to have Argentinean hemorrhagic fever. Human encephalitis cases were reported sporadically until 2005, when an unprecedented outbreak of SLE was reported in Córdoba City, Argentina (22). During this outbreak, signs and symptoms associated with a neurologic infection, including headache, sensory depression, temporal–spatial disorientation, tremors, and changes in consciousness, were reported. A correlation between age and sign or symptom severity was detected (Spearman coefficient = 0.74) (22). The frequency of encephalomyelitis varied from 80% of the cases in patients <20 years of age to 95% in those >60 years of age (22). A total of 47 probable and confirmed human cases were reported. Of these, 45 patients were hospitalized; 9 died, 1 25 years of age and 8 >50 years of age (22).

The SLEV strains CbaAr-4006 and CbaAr-4006 were isolated from Cx. pipiens quinquefasciatus mosquitoes collected during the outbreak in the backyard of the index case-patient in Córdoba City (23). Molecular classification and phylogenetic analyses indicated that the strains isolated during the outbreak were closely related to a genotype III SLEV strain (79V-2533) that had been isolated in Santa Fe Province, Argentina (23). A 3-year retrospective phylogenetic analysis of SLEV genomes from mosquitoes collected in Córdoba City, designed to find an enzootic progenitor of the outbreak strains, indicated no circulation of genotype III before the 2005 outbreak (24). The extensive sampling effort detected low levels of SLEV transmission of genotypes other than genotype III. The CbaAr-4005 strain was more virulent and produced higher viremias in avian and murine models. In house sparrows, CbaAr-4005 produced viremias that were 2.2 logs higher and lasted for 2 days longer than its closest relative, the 1978 79V-2533 strain. Because mosquitoes show a dose response to infection, house sparrows inoculated with SLEV strain CbaAr-4005 will theoretically produce viremia sufficient to infect 10 times more mosquitoes than those inoculated with a nonepidemic strain (25).

Figure 2

Thumbnail of Phylogeny and spread of St. Louis encephalitis virus (SLEV) in the Americas. A) Multiple sequence alignment of 44 complete envelope protein SLEV sequences obtained from GenBank. The orange highlighted cluster contained the emerging SLEV strains isolated in Argentina, Brazil, and western United States. Alignment was performed by ClustalX, followed by tree generation using a neighbor-joining algorithm using MEGA 6 software (https://www.megasoftware.net). Sequences are labeled by their

Figure 2. Phylogeny and spread of St. Louis encephalitis virus (SLEV) in the Americas. A) Multiple sequence alignment of 44 complete envelope protein SLEV sequences obtained from GenBank. The orange highlighted cluster contained...

In adult Swiss mice, the CbaAr-4005 strain of SLEV resulted in 100% (10/10) illness and death compared with sympatric nonepidemic SLEV strains isolated previously in Córdoba and Santa Fe Province, Argentina (26). Inoculation of only 1 plaque-forming unit in 10-day-old mice or 2 plaque-forming units in 21-day-old mice caused a 50% death rate with the strain CbaAr-4005 (lethal dose 50 [LD50] in 10-day-old mice = 0.02), proving it to be the most lethal strain compared in the study (78V-6507LD50 = 1.75; CorAn-9275LD50 = 3.90). That evidence supports the hypothesis that a more virulent SLEV strain was introduced into Córdoba City in early 2005 from Santa Fe (Argentina) or São Paulo (Brazil) (Figure 2). Serologic studies of wild birds sampled before and during the 2005 outbreak indicated that 99% (434/437) of the avian population lacked a SLEV-neutralizing antibody and were therefore susceptible to infection with SLEV, potentially serving as amplification hosts (27). The absence of natural immunity in the wild avian population may have promoted the 2005 SLE outbreak in Córdoba City. A host competence study in avian species showed that the eared dove (Zenaida auriculata) and picui ground-dove (Columbina picui) produce high levels of viremia compared with other avian species tested (28). Moreover, that study confirmed the role as amplifying host of these 2 doves species during the 2005 SLEV outbreak in Córdoba. Additional studies indicate that the population of eared doves has been increasing in the central region of Argentina during the past 10 years because of agricultural geographic expansion (29).

After the 2005 outbreak, additional SLE outbreaks in Argentina occurred in Parana (2006), Buenos Aires (2010), and San Juan (2011) (Pan American Health Organization, http://www.paho.org/hq/dmdocuments/2010/alert_epi_2010_31_marz_encefalitis_san_luis.pdf). Unfortunately, no ecologic studies focused on these outbreaks. Phylogenetic analysis of SLEV genomes associated with human cases in Buenos Aires confirmed the presence of SLEV genotype III (30).

SLE was diagnosed in a febrile human in São Paulo State, Brazil, in 2004, by viral isolation and molecular detection (31). Molecular characterization classified the isolate as SLEV genotype III. During a dengue virus outbreak in São José do Rio Preto (São Paulo State, Brazil) in 2006, Mondini et al. (32) reported the first outbreak of SLE reported in Brazil. Human cases were diagnosed by molecular detection of SLEV RNA in serum or cerebrospinal fluid. All SLEV-infected patients (6) had an initial diagnosis of dengue fever or viral encephalitis; 3 cases were diagnosed as viral meningoencephalitis, and the other 3 patients had signs of hemorrhagic disease (32). This finding was the first reported link of SLE infection and human hemorrhagic disease (32). A blastn analysis (https://blast.ncbi.nlm.nih.gov/Blast.cgi) comparing genomes of different SLEV strains, which we carried out for this review, indicates that the SLEV strains from Brazil are closely related to SLEV genotype V. This finding indicates that genotype V SLEV strains are also pathogenic for humans, as shown in previous studies in murine models (26). Because genotype V strains are widespread in South America, human SLEV cases could be misdiagnosed as dengue virus infection. A detailed analysis of South America human SLE cases is shown in Figure 1.

Reemergence in Western United States

SLEV was recognized in 1937 in California; cases were documented during 1940–1950 and in Kern County during a 1952 outbreak of Western equine encephalitis virus (33). SLEV caused periodic epidemics in humans and epizootics in equines during the 1950s–1990s. However, expansive epidemics similar to those observed in Missouri and Texas were not detected in California, although concurrent activity in mosquitoes and seroconversions in birds were repeatedly documented in the San Joaquin Valley, Los Angeles Basin, and Imperial Valley (1,34,35,36). An outbreak resulting in 26 human cases occurred in 1984 in Los Angeles, (34), leading to avian studies (37), vector competence experiments (38), and vectorial capacity studies (39). A SLEV serosurvey following the Los Angeles outbreak found that 1.6% of 1,803 serum samples tested from residents of Los Angeles were seropositive. A subsequent outbreak of 26 confirmed SLEV cases was centered in Kern County but disappeared the following summer (35,36). Envelope gene sequencing from mosquito isolates suggested that different SLEV strains were most likely responsible for these outbreaks (40). Sequencing of longitudinal SLEV isolates from California showed that isolates were genetically similar from 1952 through the 1970s, after which genetic changes were observed (41).

Even in the absence of epidemics since 1989, SLEV activity was documented continuously in California from the 1990s through 2003 by human cases, detections in mosquitoes, or seroconversions in sentinel chickens (42). In 2003, WNV activity was first detected in the state (17). During 2003–2015, no SLEV activity was detected in California, despite ongoing SLEV surveillance in Culex mosquitoes and sentinel chicken seroconversions (17). The absence of SLEV activity during that period was likely not the result of a lack of surveillance because the invasion of WNV led to a ≈6-fold statewide increase in mosquito pool testing, from ≈5,000 pools in 2003 to 30,000–35,000 pools annually since 2007. Instead, the absence of SLEV activity suggested extirpation from California.

Beginning in July 2015, SLEV activity was detected by the presence of viral RNA in mosquito pools and sentinel chicken seroconversions in the Coachella Valley of Riverside County, California (43). In 2016, SLEV spread northward to 6 additional counties in California (44); in 2017, a total of 15 California counties reported activity (45). A clinical study used unbiased clinical testing by metagenomic next-generation sequencing to diagnose a fatal case of meningoencephalitis from SLEV in a patient from Kern County, California, in September 2016 (46).

In Arizona, SLEV detection was historically less frequent than in California; low enzootic activity was reported most years during 1972–2006 and only a single human case during 2010–2014 (47). In Maricopa County (which includes Phoenix), a human SLEV outbreak during July–October 2015 resulted in 23 confirmed cases (48). Three patients in that outbreak were organ transplant recipients who experienced fever, rigors, diarrhea, headache, and confusion; all developed meningoencephalitis, and 1 patient died (48). SLEV infection in the 2 surviving transfusion recipients was confirmed by plaque reduction neutralization tests (48). Retrospective testing of archived mosquito pools from Phoenix collected in 2014 revealed a single SLEV isolate, indicating that SLEV was present in Arizona the summer before the 2015 outbreak (48).

To define the genetic relatedness of reemerging SLEV from Arizona and California to SLEV from elsewhere to infer a possible origin and pattern of spread, we performed phylogenetic analyses of genomes from mosquitoes in 2014–2016 and the fatal case in 2016 in Kern County. The 2014 and 2015 California and Arizona SLEV isolates share >99% nucleotide identity with each other and also with their closest published relative isolated from Cx. pipiens quinquefasciatus mosquitoes collected in the 2005 epidemic in Córdoba City (49). The SLEV genome sequence from the fatal case in Kern County from September 2016 was >99% identical with 2014–2015 SLEV isolates from mosquitoes in California and Arizona, suggesting that the patient was infected by the reemergent genotype circulating in the southwestern United States (46). The 2014 and 2015 SLEV isolates are genetically distinct from the 2003 Imperial Valley, California, strain that was isolated before the 11-year absence of SLEV activity in the state (49). These results suggest there was likely a single introduction of SLEV into the United States from South America, and possibly Argentina, no later than November 2014, the earliest dated sample from which SLEV was isolated in Arizona and that the virus spread in the summer of 2015 from Arizona to California (49).

Conclusion and Future Perspectives

Arthropodborne virus infections are emerging and reemerging infectious diseases worldwide. Mosquitoborne viruses, including dengue (emerged in 1990), WNV (emerged in 1999), chikungunya (2013), and Zika viruses (2015), have emerged as public health threats in the Western Hemisphere. SLEV is maintained in a bird–mosquito transmission network involving multiple species in a broad range of ecosystems encompassing a wide geographic distribution that ranges from southern Canada to southern Argentina. Biotic factors, including vector and host abundance and population age structure, as well as abiotic factors, including rainfall and drought dynamics and elevated summer temperatures, combine to produce conditions favorable for transmission of SLEV. A better understanding of how SLEV circulates between enzootic transmission cycles in nature and epidemic transmission in human populations is needed to more accurately predict where and when human SLEV epidemics will emerge.

The reemergence of SLEV in central Argentina is associated with several factors, including the recent introduction of a more virulent strain of SLEV into a naive bird community and increased populations of eared doves, a highly susceptible amplification species in Argentina. Argentina has experienced intense land use changes primarily because of the expansion of agricultural and urbanized habitats. More research is needed to define the effects of environmental change on avian reservoir and vector populations to clarify the dynamics of SLEV transmission, introduction, reintroduction, and reemergence in susceptible habitats throughout the Western Hemisphere.

The reemergence of SLEV in California and Arizona resulted from introduction of a South American strain of SLEV. The genetic diversity of SLEV in the Americas is spatially influenced, with wide genetic variation across the space, but some SLEV strains from North and South America show high genetic similarity, indicating long-range dispersal. Similar to WNV, long-range SLEV dispersal is likely mediated by migrating SLEV-infected birds. A better understanding of SLEV in wild birds and avian host migration patterns is necessary to predict the spread of SLEV.

Dr. Diaz, a biologist, is an adjunct researcher at the National Council for Scientific and Technical Researches (CONICET) and associate professor at the Instituto de Virología Dr. J. M. Vanella, Universidad Nacional de Córdoba, Argentina, where he is in charge of the Arbovirus Laboratory. His research interests include ecology and phylogeography of viral zoonoses and biological interactions among vectors, hosts, and viruses.

Top

References

  1. Reisen  WK. Epidemiology of St. Louis encephalitis virus. Adv Virus Res. 2003;61:13983. DOIPubMed
  2. Rodrigues  SG, Nunes  MR, Casseb  SM, Prazeres  AS, Rodrigues  DS, Silva  MO, et al. Molecular epidemiology of Saint Louis encephalitis virus in the Brazilian Amazon: genetic divergence and dispersal. J Gen Virol. 2010;91:24207. DOIPubMed
  3. Chamberlain  RW. History of St. Louis encephalitis. In: Monath TP, editor. St. Louis encephalitis. Washington (DC): American Public Health Association; 1980. p. 680.
  4. Monath  TP, Tsai  TF. Flaviviruses. In: Richman DD, Whitley RJ, Hayden FG, editors. Clinical Virology. New York: Churchill-Livingstone; 1997. p. 1133–86.
  5. Day  JF, Stark  LM. Frequency of Saint Louis encephalitis virus in humans from Florida, USA: 1990-1999. J Med Entomol. 2000;37:62633. DOIPubMed
  6. Day  JF, Curtis  GA. Blood feeding and oviposition byCulex nigripalpus (Diptera: Culicidae) blood feeing and oviposition before, during and after a widespread St. Louis encephalitis epidemic in Florida. J Med Entomol. 1999;36:17681. DOIPubMed
  7. Day  JF. Predicting St. Louis encephalitis virus epidemics: lessons from recent, and not so recent, outbreaks. Annu Rev Entomol. 2001;46:11138. DOIPubMed
  8. Shaman  J, Day  JF, Stieglitz  M. Drought-induced amplification of Saint Louis encephalitis virus, Florida. Emerg Infect Dis. 2002;8:57580. DOIPubMed
  9. Epstein  PR, Defilippo  C. West Nile virus and drought. Glob Change Hum Health. 2001;2:1057. DOI
  10. Curtis  GA. Habitat selection strategies of mosquitoes inhabiting citrus irrigation furrows. J Am Mosq Control Assoc. 1985;1:16973.PubMed
  11. Day  JF, Curtis  GA. When it rains, they soar—and that makes Culex nigripalpus a dangerous mosquito. Am Entomol. 1994;40:1627. DOI
  12. Fang  Y, Reisen  WK. Previous infection with West Nile or St. Louis encephalitis viruses provides cross protection during reinfection in house finches. Am J Trop Med Hyg. 2006;75:4805. DOIPubMed
  13. Ottendorfer  CL. Impact of the West Nile virus on the natural history of St. Louis encephalitis virus in Florida [dissertation]. Tampa (FL): University of South Florida; 2008 [cited 2018 Oct 2]. https://scholarcommons.usf.edu/cgi/viewcontent.cgi?referer=https://www.google.com/&httpsredir=1&article=1437&context=etd
  14. Day  JF, Stark  LM. Avian serology in a St. Louis encephalitis epicenter before, during, and after a widespread epidemic in south Florida, USA. J Med Entomol. 1999;36:61424. DOIPubMed
  15. Komar  N, Langevin  S, Hinten  S, Nemeth  N, Edwards  E, Hettler  D, et al. Experimental infection of North American birds with the New York 1999 strain of West Nile virus. Emerg Infect Dis. 2003;9:31122. DOIPubMed
  16. Reisen  WK, Chiles  RE, Martinez  VM, Fang  Y, Green  EN. Experimental infection of California birds with western equine encephalomyelitis and St. Louis encephalitis viruses. J Med Entomol. 2003;40:96882. DOIPubMed
  17. Reisen  WK, Lothrop  HD, Wheeler  SS, Kennsington  M, Gutierrez  A, Fang  Y, et al. Persistent West Nile virus transmission and the apparent displacement St. Louis encephalitis virus in southeastern California, 2003-2006. J Med Entomol. 2008;45:494508.PubMed
  18. Centers for Disease Control and Prevention. Saint Louis encephalitis; 2015 [cited 2018 Jul 27]. https://www.cdc.gov/sle/technical/epi.html
  19. Centers for Disease Control and Prevention. Saint Louis encephalitis virus: epidemiology and geographic distribution; 2017 [cited 2018 Jul 27]. https://www.cdc.gov/sle/technical/epi.html
  20. Charosky  L, Baldassari  EL, Koren  F, Mettler  NE, Loizaga  C. Síndrome encefálico a posible etiología por virus San Luis. Rev Asoc Med Argent. 1968;82:2679.
  21. Mettler  NE, Casals  J. Isolation of St. Louis encephalitis virus from man in Argentina. Acta Virol. 1971;15:14854.PubMed
  22. Spinsanti  LI, Díaz  LA, Glatstein  N, Arselán  S, Morales  MA, Farías  AA, et al. Human outbreak of St. Louis encephalitis detected in Argentina, 2005. J Clin Virol. 2008;42:2733. DOIPubMed
  23. Diaz  LA, Ré  V, Almirón  WR, Farías  A, Vázquez  A, Sanchez-Seco  MP, et al. Genotype III Saint Louis encephalitis virus outbreak, Argentina, 2005. Emerg Infect Dis. 2006;12:17524. DOIPubMed
  24. Díaz  LA, Albrieu Llinás  G, Vázquez  A, Tenorio  A, Contigiani  MS. Silent circulation of St. Louis encephalitis virus prior to an encephalitis outbreak in Cordoba, Argentina (2005). PLoS Negl Trop Dis. 2012;6:e1489. DOIPubMed
  25. Diaz  LA, Nemeth  NM, Bowen  RA, Almiron  WR, Contigiani  MS. Comparison of argentinean saint louis encephalitis virus non-epidemic and epidemic strain infections in an avian model. PLoS Negl Trop Dis. 2011;5:e1177. DOIPubMed
  26. Rivarola  ME, Tauro  LB, Llinás  GA, Contigiani  MS. Virulence variation among epidemic and non-epidemic strains of Saint Louis encephalitis virus circulating in Argentina. Mem Inst Oswaldo Cruz. 2014;109:197201. DOIPubMed
  27. Diaz  LA, Quaglia  AI, Konigheim  BS, Boris  AS, Aguilar  JJ, Komar  N, et al. Activity patterns of St. Louis encephalitis and West Nile viruses in free ranging birds during a human encephalitis outbreak in Argentina. PLoS One. 2016;11:e0161871. DOIPubMed
  28. Díaz  A, Flores  FS, Quaglia  AI, Contigiani  MS. Evaluation of Argentinean bird species as amplifying hosts for St. Louis encephalitis virus (Flavivirus, Flaviviridae). Am J Trop Med Hyg. 2018;99:21621. DOIPubMed
  29. Calamari  NC, Dardanelli  S, Canavelli  SB. Variaciones en la abundancia poblacional de palomas medianas a lo largo del tiempo. INTA EEA Paraná. Serie Extensión. 2011;64:238.
  30. Valinotto  LE, Barrero  PR, Viegas  M, Alvarez López  MC, Mistchenko  AS. Molecular evidence of St. Louis encephalitis virus infection in patients in Buenos Aires, Argentina. J Clin Virol. 2012;54:34951. DOIPubMed
  31. Santos  CL, Sallum  MA, Franco  HM, Oshiro  FM, Rocco  IM. Genetic characterization of St. Louis encephalitis virus isolated from human in São Paulo, Brazil. Mem Inst Oswaldo Cruz. 2006;101:5763. DOIPubMed
  32. Mondini  A, Cardeal  IL, Lázaro  E, Nunes  SH, Moreira  CC, Rahal  P, et al. Saint Louis encephalitis virus, Brazil. Emerg Infect Dis. 2007;13:1768. DOIPubMed
  33. Reeves  WC, Hammon  WM, Longshore  WA Jr, McClure  HE, Geib  AF. Epidemiology of the arthropod-borne viral encephalitides in Kern County, California, 1943-1952. Publ Public Health Univ Calif. 1962;4:1257.PubMed
  34. Murray  RA, Habel  LA, Mackey  KJ, Wallace  HG, Peck  BA, Mora  SJ, et al. Epidemiological aspects of the 1984 St Louis encephalitis epidemic in southern California. Proc Calif Mosq Vector Control Assoc. 1985;53:59.
  35. Reisen  WK, Meyer  RP, Milby  MM, Presser  SB, Emmons  RW, Hardy  JL, et al. Ecological observations on the 1989 outbreak of St. Louis encephalitis virus in the southern San Joaquin Valley of California. J Med Entomol. 1992;29:47282. DOIPubMed
  36. Tueller  JE. An outbreak of illness due to St. Louis encephalitis virus in the southern San Joaquin Valley, California, 1989. Proc Calif Mosq Vector Control Assoc. 1990;58:12.
  37. McLean  RG, Webb  JP, Campos  EG, Gruwell  J, Francy  DB, Womeldorf  D, et al. Antibody prevalence of St. Louis encephalitis virus in avian hosts in Los Angeles, California, 1986. J Am Mosq Control Assoc. 1988;4:5248.PubMed
  38. Hardy  JL, Meyer  RP, Reisen  WK, Presser  SB. A further evaluation of Culex mosquitoes in the greater Los Angeles area for their ability to vector St. Louis encephalitis virus. Proc Calif Mosq Vector Control Assoc. 1986;54:910.
  39. Reisen  WK, Milby  MM, Presser  SB, Hardy  JL. Ecology of mosquitoes and St. Louis encephalitis virus in the Los Angeles Basin of California, 1987–1990. J Med Entomol. 1992;29:58298. DOIPubMed
  40. Reisen  WK, Lothrop  HD, Chiles  RE, Cusack  R, Green  EG, Fang  Y, et al. Persistence and amplification of St. Louis encephalitis virus in the Coachella Valley of California, 2000–2001. J Med Entomol. 2002;39:793805. DOIPubMed
  41. Kramer  LD, Presser  SB, Hardy  JL, Jackson  AO. Genotypic and phenotypic variation of selected Saint Louis encephalitis viral strains isolated in California. Am J Trop Med Hyg. 1997;57:2229. DOIPubMed
  42. Reisen  W, Lothrop  H, Chiles  R, Madon  M, Cossen  C, Woods  L, et al. West Nile virus in California. Emerg Infect Dis. 2004;10:136978. DOIPubMed
  43. Feiszli  T, Padgett  K, Simpson  J, Barker  CM, Fang  Y, Salas  M, et al. Surveillance for mosquito-borne encephalitis virus activity in California, 2015. Proc Papers Mosq Vector Control Assoc Calif. 2016;84:1249.
  44. Feiszli  T, Padgett  K, Simpson  J, Barker  CM, Fang  Y, Salas  M, et al. Surveillance for mosquito-borne encephalitis virus activity in California, 2016. Proc Papers Mosq Vector Control Assoc Calif. 2017;85:914.
  45. Saint Louis encephalitis virus (SLEV) in California counties 2017 YTD; updated Jan 2018 [cited 2018 Jul 27]. http://westnile.ca.gov/downloads.php?download_id=3839&filename=2017_slev_ county_map.pdf
  46. Chiu  CY, Coffey  LL, Murkey  J, Symmes  K, Sample  HA, Wilson  MR, et al. Diagnosis of fatal human case of St. Louis encephalitis virus infection by metagenomic sequencing, California, 2016. Emerg Infect Dis. 2017;23:19648. DOIPubMed
  47. Arizona State Public Health Laboratory. Epidemiology and disease control. 2014 [cited 2018 Jul 27]. https://www.azdhs.gov/preparedness/epidemiology-disease-control/index.php.
  48. Venkat  H, Krow-Lucal  E, Hennessey  M, Jones  J, Adams  L, Fischer  M, et al. Concurrent outbreaks of St. Louis encephalitis virus and West Nile virus disease—Arizona, 2015. MMWR Morb Mortal Wkly Rep. 2015;64:134950.PubMed
  49. White  GS, Symmes  K, Sun  P, Fang  Y, Garcia  S, Steiner  C, et al. Reemergence of St. Louis encephalitis virus, California, 2015. Emerg Infect Dis. 2016;22:21858. DOIPubMed

Top

Figures

Top

Follow Up

Earning CME Credit

To obtain credit, you should first read the journal article. After reading the article, you should be able to answer the following, related, multiple-choice questions. To complete the questions (with a minimum 75% passing score) and earn continuing medical education (CME) credit, please go to http://www.medscape.org/journal/eid. Credit cannot be obtained for tests completed on paper, although you may use the worksheet below to keep a record of your answers.

You must be a registered user on http://www.medscape.org. If you are not registered on http://www.medscape.org, please click on the “Register” link on the right hand side of the website.

Only one answer is correct for each question. Once you successfully answer all post-test questions, you will be able to view and/or print your certificate. For questions regarding this activity, contact the accredited provider, CME@medscape.net. For technical assistance, contact CME@medscape.net. American Medical Association’s Physician’s Recognition Award (AMA PRA) credits are accepted in the US as evidence of participation in CME activities. For further information on this award, please go to https://www.ama-assn.org. The AMA has determined that physicians not licensed in the US who participate in this CME activity are eligible for AMA PRA Category 1 Credits™. Through agreements that the AMA has made with agencies in some countries, AMA PRA credit may be acceptable as evidence of participation in CME activities. If you are not licensed in the US, please complete the questions online, print the AMA PRA CME credit certificate, and present it to your national medical association for review.

Article Title: Reemergence of St. Louis Encephalitis Virus in the Americas

CME Questions

1. You are advising a public health department in California regarding potential needs for St. Louis encephalitis virus (SLEV) detection and management. On the basis of the epidemiological review by Diaz and colleagues, which one of the following statements about the history of SLEV epidemiology in the United States is correct?

A. SLEV was first identified as the causative agent of a mosquito-borne viral epidemic in St. Louis, Missouri, during the summer of 1953

B. SLEV activity increased considerably in the United States after West Nile virus introduction in 1999

C. SLEV genotypes IV and V are most prevalent in the United States D. SLEV is transmitted by numerous Culex mosquito species, and is amplified by passerine and columbiform avian species

2. According to the epidemiological review by Diaz and colleagues, which one of the following statements about the emergence and reemergence of SLEV in South America is correct?

A. Introduction of a more pathogenic strain and increasing populations of the eared dove as amplifying host led to the emergence of SLEV in Argentina

B. Human SLE epidemics have occurred every 2 to 3 years in Latin America since the 1980s

C. The 2005 outbreak of SLE in Córdoba City had worse outcomes in younger patients

D. SLEV strains isolated during the 2005 outbreak were closely related to a genotype V SLEV strain

3. On the basis of the epidemiological review by Diaz and colleagues, which one of the following statements about reemergence of SLEV in the western United States is correct?

A. In 2014 to 2015, SLEV caused a human outbreak in New Mexico

B. Emerging SLEV in the western United States is related to the strains isolated during sporadic transmission in Florida in 1993 and 1997

C. Reemerging SLEV in the western United States may have been mediated via migrating infected birds from southern South America, where it reemerged most recently in 2002

D. Longitudinal SLEV isolates from California were genetically similar from 1952 through 2015

Top

Cite This Article

DOI: 10.3201/eid2412.180372

Original Publication Date: 12/3/2018

Table of Contents – Volume 24, Number 12—December 2018

Comments

Please use the form below to submit correspondence to the authors or contact them at the following address:

Adrián Diaz, National University of Córdoba–Arbovirus Laboratory, Institute of Virology Dr. J.M. Vanella, School of Medicine, Enfermera Gordillo Gómez s/n, CPX5016GCA, Córdoba, Argentina


character(s) remaining.

Comment submitted successfully, thank you for your feedback.

Top

TOP