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Volume 24, Number 2—February 2018
Research

Plasmid-Encoded Transferable mecB-Mediated Methicillin Resistance in Staphylococcus aureus

Karsten BeckerComments to Author , Sarah van Alen, Evgeny A. Idelevich, Nina Schleimer, Jochen Seggewiß, Alexander Mellmann, Ursula Kaspar, and Georg Peters
Author affiliations: University Hospital Münster, Münster, Germany (K. Becker, S. van Alen, E.A. Idelevich, N. Schleimer, J. Seggewiß, A. Mellmann, U. Kaspar, G. Peters); Cells in Motion, Münster (G. Peters)

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Table 1

Oligonucleotides used in study of methicillin resistance genes in Staphylococcus aureus

Gene Oligonucleotide Nucleotide sequence, 5′ → 3′ Melting temperature Source
mecA
mec5 AAAATCGATGGTAAAGGTTGGC 55.5°C
(29)
mec6
AGTTCTGCAGTACCGGATTTGC
mecC
mecAL3 TCAAATTGAGTTTTTCCATTATCA 59.3°C
This study
mecAL4
AACTTGGTTATTCAAAGATGACGA
mecB mecB-for TTAACATATACACCCGCTTG 57°C This study
mecB-rev TAAAGTTCATTAGGCACCTCC

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