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Volume 24, Number 3—March 2018
Research

Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry1

Viktor MånssonComments to Author , Janet R. Gilsdorf, Gunnar Kahlmeter, Mogens Kilian, J. Simon Kroll, Kristian Riesbeck2, and Fredrik Resman2
Author affiliations: Lund University, Malmö, Sweden (V. Månsson, K. Riesbeck, F. Resman); University of Michigan, Ann Arbor, Michigan, USA (J.R. Gilsdorf); Växjö Hospital, Växjö, Sweden (G. Kahlmeter); Aarhus University, Aarhus, Denmark (M. Kilian); Imperial College London, London, UK (J.S. Kroll)

Main Article

Figure 1

Culture collections and methods used in this study for capsule typing of Haemophilus influenzae by MALDI-TOF mass spectrometry. An evaluation set of H. influenzae isolates of all capsule types from diverse geographic origins and time periods and isogenic capsule transformants (30) were used to investigate capsule type-specific differences in MALDI-TOF mass spectra. MLST was used to ensure adequate coverage of different genetic lineages of encapsulated H. influenzae. Reference isolates from the e

Figure 1. Culture collections and methods used in this study for capsule typing of Haemophilus influenzae by MALDI-TOF mass spectrometry. An evaluation set of H. influenzae isolates of all capsule types from diverse geographic origins and time periods and isogenic capsule transformants (30) were used to investigate capsule type-specific differences in MALDI-TOF mass spectra. MLST was used to ensure adequate coverage of different genetic lineages of encapsulated H. influenzae. Reference isolates from the evaluation set (encapsulated and nonencapsulated) were selected to construct a new typing database in MALDI Biotyper. This database was tested with the remaining isolates in the set, and misclassified isolates were added to the database. The final supplemented database was blindly validated with a second culture collection that consisted of clinical invasive isolates. Hia, H. influenzae type a; Hib, H. influenzae type b; Hic, H. influenzae type c; Hid, H. influenzae type d; Hie, H. influenzae type e; Hif, H. influenzae type f; MALDI-TOF, matrix-assisted laser desorption/ionization time-of-flight; MLST, multilocus sequence typing; NTHi, nontypeable H. influenzae.

Main Article

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Main Article

1Preliminary results from this study were presented at the IDWeek 2016 Conference, October 26−30, 2016, New Orleans, Louisiana, USA.

2These senior authors contributed equally to this article.

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