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Volume 24, Number 6—June 2018
Dispatch

Mixed Leptospira Infections in a Diverse Reservoir Host Community, Madagascar, 2013–2015

Mark MoseleyComments to Author , Soanandrasana Rahelinirina, Minoarisoa Rajerison, Benoit Garin, Stuart Piertney, and Sandra Telfer
Author affiliations: University of Aberdeen, Aberdeen, Scotland, UK (M. Moseley, S. Piertney, S. Telfer); Institut Pasteur, Antananarivo, Madagascar (S. Rahelinirina, M. Rajerison, B. Garin); Centre Hospitalier Universitaire, Les Abymes, Guadeloupe (B. Garin)

Main Article

Figure

Proportion of Leptospira-positive Rattus rattus rat samples (n = 63) infected with L. borgpetersenii, L. interrogans, or L. mayottensis before and after characterizing mixed infections, Madagascar, 2013–2015. We initially genotyped R. rattus rat samples positive for Leptospira 16S rRNA by sequencing ≈300 bp of the lfb1 gene using standard primers and thermal profile (6). To characterize mixed infections, we used forward primers targeting the lfb1 locus of the different Leptospira species and the

Figure. Proportion of Leptospira-positive Rattus rattus rat samples (n = 63) infected with L. borgpetersenii, L. interrogans, or L. mayottensis before and after characterizing mixed infections, Madagascar, 2013–2015. We initially genotyped R. rattus rat samples positive for Leptospira 16S rRNA by sequencing ≈300 bp of the lfb1 gene using standard primers and thermal profile (6). To characterize mixed infections, we used forward primers targeting the lfb1 locus of the different Leptospira species and the standard reverse primer and thermal profile. Mixed infections result in the sum of proportions exceeding 100% after characterization. Error bars represent 95% CIs.

Main Article

Page created: May 17, 2018
Page updated: May 17, 2018
Page reviewed: May 17, 2018
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