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Volume 24, Number 7—July 2018

Dispatch

Use of Urea Wash ELISA to Distinguish Zika and Dengue Virus Infections

Wen-Yang Tsai, Han Ha Youn, Jasmine Tyson, Carlos Brites, Jih-Jin Tsai, Celia Pedroso, Jan Felix Drexler, Angel Balmaseda, Eva Harris, and Wei-Kung WangComments to Author 
Author affiliations: University of Hawaii at Manoa, Honolulu, Hawaii, USA (W.-Y. Tsai, H.H. Youn, J. Tyson, W.-K. Wang); Federal University of Bahia, Salvador, Brazil (C. Brites, C. Pedroso); Kaohsiung Medical University, Kaohsiung, Taiwan (J.-J. Tsai); University of Bonn Medical Centre, Bonn, Germany (J.F. Drexler); National Center for Diagnosis and Reference, Ministry of Health, Managua, Nicaragua (A. Balmaseda); University of California at Berkeley, Berkeley, California, USA (E. Harris)

Main Article

Figure 1

NS1 IgM and IgG ELISAs and urea wash in ZIKV–NS1 IgG ELISA. A) Positivity rates for each panel. Only samples collected <3 months after symptom onset were tested for IgM. B) sDENV infection and probable DENV-ZIKV panels were tested with different concentrations (4, 6, and 8 mol/L) of urea wash. C, D) sDENV and DENV-ZIKV panels were tested with 6 mol/L urea wash: C) all samples; D) samples positive for both DENV-1–NS1 and ZIKV-NS1 IgG ELISAs. Sensitivity and specificity are based on relative op

Figure 1. NS1 IgM and IgG ELISAs and urea wash in ZIKV–NS1 IgG ELISA. A) Positivity rates for each panel. Only samples collected <3 months after symptom onset were tested for IgM. B) sDENV infection and probable DENV-ZIKV panels were tested with different concentrations (4, 6, and 8 mol/L) of urea wash. C, D) sDENV and DENV-ZIKV panels were tested with 6 mol/L urea wash: C) all samples; D) samples positive for both DENV-1–NS1 and ZIKV-NS1 IgG ELISAs. Sensitivity and specificity are based on relative optical density cutoff at 0.28 (dashed line). Receiver-operating characteristics are shown in the graph on the right. Data are the mean of 2 experiments (each in duplicate). The 2-tailed Mann-Whitney test was used. DENV, dengue virus; DENV-ZIKV, confirmed Zika virus infection with previous exposure to DENV; NS1, nonstructural protein 1; pDENV1, primary DENV-1 infection; pZIKV, primary ZIKV infection; rOD, relative optical density; sDENV, secondary DENV infection; ZIKV, Zika virus.

Main Article

Page created: June 18, 2018
Page updated: June 18, 2018
Page reviewed: June 18, 2018
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