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Volume 24, Number 7—July 2018
Dispatch

Use of Urea Wash ELISA to Distinguish Zika and Dengue Virus Infections

Wen-Yang Tsai, Han Ha Youn, Jasmine Tyson, Carlos Brites, Jih-Jin Tsai, Celia Pedroso, Jan Felix Drexler, Angel Balmaseda, Eva Harris, and Wei-Kung WangComments to Author 
Author affiliations: University of Hawaii at Manoa, Honolulu, Hawaii, USA (W.-Y. Tsai, H.H. Youn, J. Tyson, W.-K. Wang); Federal University of Bahia, Salvador, Brazil (C. Brites, C. Pedroso); Kaohsiung Medical University, Kaohsiung, Taiwan (J.-J. Tsai); University of Bonn Medical Centre, Bonn, Germany (J.F. Drexler); National Center for Diagnosis and Reference, Ministry of Health, Managua, Nicaragua (A. Balmaseda); University of California at Berkeley, Berkeley, California, USA (E. Harris)

Main Article

Figure 2

NS1 IgG ELISAs with urea wash for sDENV infection panel over time. A) DENV-1–NS1 IgG ELISA; B) ZIKV–NS1 IgG ELISA; and C) ZIKV-NS1 IgG ELISA with 6 mol/L urea wash for sDENV samples collected from 3 months to 6 years after symptom onset. D) Sequentially collected samples from 3 patients with sDENV infection. Dotted lines indicate relative optical density cutoffs of ELISAs; dashed lines indicate rOD cutoff (0.28) of ELISA with 6 mol/L urea wash. Data are expressed as mean ± SD (for panel D) of 2

Figure 2. NS1 IgG ELISAs with urea wash for sDENV infection panel over time. A) DENV-1–NS1 IgG ELISA; B) ZIKV–NS1 IgG ELISA; and C) ZIKV-NS1 IgG ELISA with 6 mol/L urea wash for sDENV samples collected from 3 months to 6 years after symptom onset. D) Sequentially collected samples from 3 patients with sDENV infection. Dotted lines indicate relative optical density cutoffs of ELISAs; dashed lines indicate rOD cutoff (0.28) of ELISA with 6 mol/L urea wash. Data are expressed as mean ± SD (for panel D) of 2 experiments (each in duplicate). The 2-tailed Mann-Whitney test was used. DENV, dengue virus; NS1, nonstructural protein 1; rOD, relative optical density; sDENV, secondary DENV infection; ZIKV, Zika virus.

Main Article

Page created: June 18, 2018
Page updated: June 18, 2018
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