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Volume 24, Number 8—August 2018
CME ACTIVITY - Research

Ancylostoma ceylanicum Hookworm in Myanmar Refugees, Thailand, 2012–2015

Elise M. O’ConnellComments to Author , Tarissa Mitchell, Marina Papaiakovou, Nils Pilotte, Deborah Lee, Michelle Weinberg, Potsawin Sakulrak, Dilok Tongsukh, Georgiette Oduro-Boateng, Sarah Harrison, Steven A. Williams, William M. Stauffer1, and Thomas B. Nutman1
Author affiliations: National Institutes of Health, Bethesda, Maryland, USA (E.M. O’Connell, G. Oduro-Boateng, S. Harrison, T.B. Nutman); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (T. Mitchell, D. Lee, M. Weinberg, W.M. Stauffer); Smith College, Northampton, Massachusetts, USA (M. Papaiakovou, N. Pilotte, S.A. Williams); University of Massachusetts, Amherst, Masschusetts, USA (N. Pilotte, S.A. Williams); International Organization for Migration, Mae Sot, Thailand (P. Sakulrak, D. Tongsukh); University of Minnesota Medical School, Minneapolis, Minnesota, USA (W.M. Stauffer)

Main Article

Table 1

Primer-probe sets used to determine genotypes of hookworms present in US-bound Myanmar refugees in camps along Myanmar–Thailand border, Thailand, 2012–2015*

Genome target/primer-probe set name, sequence type Sequence Final concentration, nmol/L Reference
ITS2/Ad1 (23)
Forward primer 5′-GAATGACAGCAAACTCGTTGTTG-3′ 900
Reverse primer 5′-ATACTAGCCACTGCCGAAACGT-3′ 900
Probe
5′-ATCGTTTACCGACTTTAG-3′
250

Repetitive element/Ad2 (24)
Forward primer 5′-GTATTTCACTCATATGATCGAGTGTTC-3′ 900
Reverse primer 5′-GTTTGAATTTGAGGTATTTCGACCA-3′ 900
Probe
5′-TGACAGTGTGTCATACTGTGGAAA-3′
250
Repetitive element/Ac (25)
Forward primer 5′-CAAATATTACTGTGCGCATTTAGC-3′ 900
Reverse primer 5′-GCGAATATTTAGTGGGTTTACTGG-3′ 900
Probe
5′-CGGTGAAAGCTTTGCGTTATTGCGA-3′
250
Repetitive element/Na (24)
Forward primer 5′-CCAGAATCGCCACAAATTGTAT-3′ 900
Reverse primer 5′-GGGTTTGAGGCTTATCATAAAGAA-3′ 900
Probe
5′-CCCGATTTGAGCTGAATTGTCAAA-3′
250
SNP200 This paper
Forward primer 5′-AATGCTACACTCTCTGTTCACCAGTT-3′ 900
Reverse primer 5′-CGGAAGCAGATATCATACAAAGCTT-3′ 900
Wild-type FAM probe/mutant VIC probe†
5′-AATACAGATGAGACCT(T/A)CT-3′
166; 231
SNP167 This paper
Forward primer 5′-TCGGGAAGAATACCCTGATAGAAT-3′ 900
Reverse primer 5′-CTTTTGCTCTTATTTCCATCAATAGGA-3′ 900
Wild-type FAM probe/mutant VIC probe† 5′-TGTCCTCGT(T/A)TTCC-3′ 125; 350

*Ac, Ancylostoma ceylanicum set; Ad1, A. duodenale set 1; Ad2, A. duodenale set 2; ITS2, internal transcribed spacer 2; Na, Necator americanus set; SNP, single-nucleotide polymorphism.
†FAM and VIC probes (Integrated DNA Technologies, Skokie, IL, USA).

Main Article

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Main Article

1These authors contributed equally to this article

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