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Volume 24, Number 8—August 2018

Direct Detection of penA Gene Associated with Ceftriaxone-Resistant Neisseria gonorrhoeae FC428 Strain by Using PCR

David M. WhileyComments to Author , Lebogang Mhango, Amy V. Jennison, Graeme Nimmo, and Monica M. Lahra
Author affiliations: The University of Queensland, Brisbane, Queensland, Australia (D.M. Whiley, L. Mhango); Pathology Queensland Central Laboratory, Brisbane (D.M. Whiley, G. Nimmo); Queensland Health Forensic and Scientific Services, Archerfield, Queensland, Australia (A.V. Jennison); Griffith University, Gold Coast, Queensland, Australia (G. Nimmo); The Prince of Wales Hospital Randwich, Sydney, New South Wales, Australia (M.M Lahra); The University of New South Wales, Sydney (M.M. Lahra)

Main Article

Table 1

Primer and probe sequences for PCR to detect Neisseria gonorrhoeae FC428 strain*

Designation Oligonucleotide sequence, 5′ → 3′
Probe 1 Fam-TTCA+T+G+A+CA+G+AAC-Iowa Black FQ
Probe 2 Hex-TCA+T+G+G+CA+GA-Iowa Black FQ

*LNA bases are indicated by + preceding the base in the sequence.

Main Article

Page created: July 18, 2018
Page updated: July 18, 2018
Page reviewed: July 18, 2018
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