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Volume 24, Number 8—August 2018

Dispatch

Direct Detection of penA Gene Associated with Ceftriaxone-Resistant Neisseria gonorrhoeae FC428 Strain by Using PCR

David M. WhileyComments to Author , Lebogang Mhango, Amy V. Jennison, Graeme Nimmo, and Monica M. Lahra
Author affiliations: The University of Queensland, Brisbane, Queensland, Australia (D.M. Whiley, L. Mhango); Pathology Queensland Central Laboratory, Brisbane (D.M. Whiley, G. Nimmo); Queensland Health Forensic and Scientific Services, Archerfield, Queensland, Australia (A.V. Jennison); Griffith University, Gold Coast, Queensland, Australia (G. Nimmo); The Prince of Wales Hospital Randwich, Sydney, New South Wales, Australia (M.M Lahra); The University of New South Wales, Sydney (M.M. Lahra)

Main Article

Table 2

Neisseria spp. isolates and specimens tested in development of PCR to detect Neisseria gonorrhoeae FC428 strain*

Isolates/samples
 PCR results for FC428 (Ct)
Probe 1
 Probe 2
Gonococcal species, n = 144
Neisseria gonorrhoeae, n = 72
N. gonorrhoeae FC428, n = 2 (9)† Positive (19.8 and 18.17 cycles) Negative
N. gonorrhoeae H041, n = 1 (1) Negative Negative
N. gonorrhoeae A8806, n = 1 (4) Positive (37. 8 cycles) Negative
N. gonorrhoeae, n = 68‡
Negative
 Negative
Nongonococcal species, n = 111
N. cinerea, n = 4 Negative Negative
N. elongata, n = 1 Negative Negative
N. flavescens, n = 1 Negative Negative
N. lactamica, n = 15 Negative Negative
N. lactamica, n = 1 Positive (42.8 cycles) Negative
N. meningitidis, n = 55 Negative Negative
N. meningitidis, n = 1 Negative Positive (32.6 cycles)
N. mucosa, n = 1 Negative Negative
N. polysacchareae, n = 4 Negative Negative
N. sicca, n = 4 Negative Negative
N. subflava, n = 14 Negative Negative
N. weaveri, n = 1 Negative Negative
Moraxella catarrhalis, n = 7 Negative Negative
M. osloensis, n = 2
 Negative
 Negative
N. gonorrhoeae NAAT-positive clinical specimens, n = 358
Urogenital, n = 172 Negative Negative
Anal swab, n = 81 Negative Negative
Throat swab, n = 95 Negative Negative
Other, n = 10 Negative Negative

*Ct, cycle threshold; NAAT, nucleic acid amplification test.
†Isolates A7846 and A7536.
‡Other local clinical isolates collected in New South Wales, Australia.

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Page updated: July 18, 2018
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