Volume 24, Number 8—August 2018
Dispatch
Direct Detection of penA Gene Associated with Ceftriaxone-Resistant Neisseria gonorrhoeae FC428 Strain by Using PCR
Table 2
Neisseria spp. isolates and specimens tested in development of PCR to detect Neisseria gonorrhoeae FC428 strain*
Isolates/samples |
PCR results for FC428 (Ct) | |
---|---|---|
Probe 1 |
Probe 2 |
|
Gonococcal species, n = 144 | ||
Neisseria gonorrhoeae, n = 72 | ||
N. gonorrhoeae FC428, n = 2 (9)† | Positive (19.8 and 18.17 cycles) | Negative |
N. gonorrhoeae H041, n = 1 (1) | Negative | Negative |
N. gonorrhoeae A8806, n = 1 (4) | Positive (37. 8 cycles) | Negative |
N. gonorrhoeae, n = 68‡ |
Negative |
Negative |
Nongonococcal species, n = 111 | ||
N. cinerea, n = 4 | Negative | Negative |
N. elongata, n = 1 | Negative | Negative |
N. flavescens, n = 1 | Negative | Negative |
N. lactamica, n = 15 | Negative | Negative |
N. lactamica, n = 1 | Positive (42.8 cycles) | Negative |
N. meningitidis, n = 55 | Negative | Negative |
N. meningitidis, n = 1 | Negative | Positive (32.6 cycles) |
N. mucosa, n = 1 | Negative | Negative |
N. polysacchareae, n = 4 | Negative | Negative |
N. sicca, n = 4 | Negative | Negative |
N. subflava, n = 14 | Negative | Negative |
N. weaveri, n = 1 | Negative | Negative |
Moraxella catarrhalis, n = 7 | Negative | Negative |
M. osloensis, n = 2 |
Negative |
Negative |
N. gonorrhoeae NAAT-positive clinical specimens, n = 358 | ||
Urogenital, n = 172 | Negative | Negative |
Anal swab, n = 81 | Negative | Negative |
Throat swab, n = 95 | Negative | Negative |
Other, n = 10 | Negative | Negative |
*Ct, cycle threshold; NAAT, nucleic acid amplification test.
†Isolates A7846 and A7536.
‡Other local clinical isolates collected in New South Wales, Australia.
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