Volume 25, Number 10—October 2019
Early Diagnosis of Tularemia by Flow Cytometry, Czech Republic, 2003–20151
|Median||95% CI||Interquartile range||Range|
|Time relative to onset of patient symptoms|
|Diagnostic test type|
|Flow cytometry, n = 58||18.5||15.5–22.0||9.75–33.25||2–128|
|Serologic test, n = 58
|Time to first positive serologic test result relative to rise in CD3+/CD4–/CD8– T cells|
|All, n = 58||7.0||1.0–12.0||0–18.75||–50 to 62|
|Delayed seroconverters, n = 34||14.0||8.0–22.0||7.5–22.0||1–62|
*A positive flow cytometry test result for tularemia was defined as >8% of peripheral blood CD3+ T cells having the CD4–/CD8– phenotype. A positive serologic test result for tularemia included probable and confirmed diagnoses and was defined for probable cases as an antibody titer of >1:20 in any acute phase blood sample or for confirmed cases as an antibody titer of >1:160 in any blood sample or a seroconversion from negative to positive (any titer) or a 4-fold increase in titer between acute and convalescent patient samples (agglutination test; Tularemia Diagnostic Set, Bioveta a.s., https://www.bioveta.eu).
1Preliminary data from this study were presented at the European Congress of Clinical Microbiology and Infectious Diseases; April 9–12, 2016; Amsterdam, the Netherlands (abstract no. O367).