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Volume 25, Number 10—October 2019
Emergence of Influenza A(H7N4) Virus, Cambodia
Active surveillance in high-risk sites in Cambodia has identified multiple low-pathogenicity influenza A(H7) viruses, mainly in ducks. None fall within the A/Anhui/1/2013(H7N9) lineage; however, some A(H7) viruses from 2018 show temporal and phylogenetic similarity to the H7N4 virus that caused a nonfatal infection in Jiangsu Province, China, in December 2017.
Avian influenza virus (AIV) subtype A(H7) is of concern because it has been a leading cause of zoonotic infections over the past 2 decades (1). The A/Anhui/1/2013-lineage A(H7N9) viruses, a leading cause of zoonotic infections in Asia since 2013, have not been detected in the Greater Mekong Subregion, but independent H7 lineages, including H7N3, H7N7, and H7Nx, have been detected occasionally in Cambodia since 2009 (2–4). H7N3 virus was detected from a duck mortality event in Kampong Thom during January 2017 (2), and H7N7 virus was detected in a live-bird market (LBM) in Takeo in September 2017 (4). Furthermore, highly pathogenic avian influenza (HPAI) A(H5N1) and low-pathogenicity avian influenza (LPAI) A(H9N2) are endemic in Cambodia (5); 59 poultry outbreaks of AIV and 56 human HPAI A(H5N1) cases have occurred since 2006. Although the exact ecologic links are unknown, serologic studies suggest that AIVs of multiple subtypes are frequently introduced into poultry in Cambodia, possibly through cross-border trade or through wild birds (2,6,7).
In December 2017, a 68-year-old woman in Jiangsu, China, who had underlying medical conditions was infected by an LPAI influenza A(H7N4) virus, which led to severe pneumonia and intensive care unit admission, but she recovered and left the hospital after 21 days (8,9). Genetically similar H7N4 viruses were subsequently detected in contact chickens (9,10) and aquatic poultry in Jiangsu (GISAID, https://www.gisaid.org), substantiating that the infection was zoonotic and raising concerns of endemicity of H7N4 in the region. Because of the antigenic differences between the A/Jiangsu/1/2018-like A(H7N4) virus and other H7 lineages (10), including A/Anhui/1/2013(H7N9) lineage, this newly detected H7N4 virus has been proposed as a vaccine candidate for pandemic preparedness (10).
Beginning in February 2018, 2 months after the H7N4 case in China, this virus was detected in ducks in Cambodia; the frequency of detection increased in March and April (4). Therefore, because of the novelty of the strain and the association with human infection, we sought to understand the genomic diversity of H7 viruses in Cambodia.
We characterized the whole genomes (for sequencing methods, see Appendix) of 16 viruses collected during 2015–2018 subtyped by reverse transcription PCR (RT-PCR) as having an H7 hemagglutinin (HA) gene or an N4 neuraminidase (NA) gene; we also included viruses for which the HA or NA could not be typed but that were epidemiologically associated with A(H7) viruses (Appendix Table). We obtained samples from poultry swabs collected across multiple LBMs, slaughterhouses, and poultry collection centers in Cambodia; most H7 viruses originated from domestic ducks (4).
All AIV samples collected during February–April 2018 in Cambodia (n = 9) (Appendix Table 1, Figure 1) contained >1 segment with high similarity and common evolutionary origins to the Jiangsu H7N4 samples, whereas AIV collected before this period formed other independent lineages derived from wild birds. Seven H7-HA from viruses collected in 2018 in Cambodia (4 H7N4, 1 H7N5, 1 H7Nx, and 1 H7 with mixed N4 and N7 segments) were most closely related to the HA and NA genes of Jiangsu H7N4 isolates; all 6 N4 NA were most closely related to the NA genes of Jiangsu H7N4 isolates (Figure). We also observed close relationships between the Jiangsu and Cambodia isolates in the internal segments polymerase basic protein 2 (PB2), polymerase acidic protein (PA), and nucleoprotein (NP); most viruses carryied a common PA gene (Appendix Figure 1). However, none of the H7N4 viruses from Cambodia shared all segments with Jiangsu isolates, indicating continual reassortment with AIV co-circulating in the region.
Phylogenetic analysis showed that the Cambodia–Jiangsu H7-HA genes emerged during late 2017 (mean time to most recent common ancestor November 2017; 95% CI August 2016–July 2017) and were derived from H7N7 and H7N2 viruses previously detected in aquatic birds in east Asia (Appendix Figure 2). In contrast, the N4-NA exhibited a greater diversity in Cambodia (mean time to most recent common ancestor January 2016; 95% CI January 2015–November 2016) and were derived from H10N4 and H8N4 viruses previously detected in Georgia, Russia, and Mongolia.
Our results show that H7N4 is a newly developing virus lineage that originated from divergent avian lineages within the Eurasian AIV gene pool. The dispersed genetic origins from locations in Europe and central Asia and the similarity of the Cambodia and Jiangsu H7N4 samples indicates that the H7N4 virus was generated in aquatic birds, likely just before their first detection. Detection of H7N4 in LBMs in Cambodia in such a short span of time at such a large spatial distance highlights the risk and potential for rapid spread of AIV lineages throughout the region. The ability to infect a human subject, the continual reassortment and antigenic evolution of this lineage, and the endemicity of numerous LPAI and HPAI viruses may further increase the risk for zoonotic infections and warrants vigilant, active surveillance in wild birds and poultry in the region.
Dr. Vijaykrishna is an evolutionary biologist based at the Biomedicine Discovery Institute in Melbourne, working closely with staff and students at IPC and World Health Organization Collaborating Centre, Melbourne. His primary research interests are in using disease surveillance and comparative genomics to track and solve problems in clinical and veterinary virology.
The investigators thank everyone on the Influenza Team in the Virology Unit at Institut Pasteur du Cambodge (IPC) who contributed to this study, including Viseth Srey Horm, Songha Tok, Phalla Y, Ponnarath Keo, Sereyrath Sun, Sonita Kol, Chiva Sum, Sarath Sin, Kim Lay Chea, and Sokhoun Yann. In addition, we thank Veasna Duong. We also thank all of the support teams at IPC, including the drivers and facilities personnel who make these studies possible, as well as all the local teams, epidemiologists, veterinary officers, and other staff from the National Health and Production Research Institute. We also thank everyone from the regional and country Food and Agriculture Organization of the United Nations (FAO) offices, especially Makara Hak and Aurelie Brioudes.
Work at Institut Pasteur in Cambodia was supported the Office of the Assistant Secretary for Preparedness and Response, US Department of Health and Human Services (grant no. IDSEP 140020-01-00; http://www.asideproject.org), and by the FAO, with funding from the United States Agency for International Development (USAID) under the Emerging Pandemics Threats 2 (EPT-2) project. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health. V.D. and M.L.G. are supported by contract no. HHSN272201400006C from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, US Department of Health and Human Services.
- Abdelwhab EM, Veits J, Mettenleiter TC. Prevalence and control of H7 avian influenza viruses in birds and humans. Epidemiol Infect. 2014;142:896–920.
- Suttie A, Yann S, Y P, Tum S, Deng YM, Hul V, et al. Detection of low pathogenicity influenza A(H7N3) virus during duck mortality event, Cambodia, 2017. Emerg Infect Dis. 2018;24:1103–7.
- World Health Organization. Cumulative number of confirmed human cases for avian influenza A(H5N1) reported to WHO, 2003–2018. 2018 [cited 2018 Dec 13]. https://www.who.int/influenza/human_animal_interface/H5N1_cumulative_table_archives
- Karlsson EA, Horm SV, Tok S, Tum S, Kalpravidh W, Claes F, et al. Avian influenza virus detection, temporality and co-infection in poultry in Cambodian border provinces, 2017–2018. Emerg Microbes Infect. 2019;8:637–9.
- Suttie A, Karlsson EA, Deng YM, Horm SV, Yann S, Tok S, et al. Influenza A(H5N1) viruses with A(H9N2) single gene (matrix or PB1) reassortment isolated from Cambodian live bird markets. Virology. 2018;523:22–6.
- Horm SV, Tarantola A, Rith S, Ly S, Gambaretti J, Duong V, et al. Intense circulation of A/H5N1 and other avian influenza viruses in Cambodian live-bird markets with serological evidence of sub-clinical human infections. Emerg Microbes Infect. 2016;5:
- Van Kerkhove MD, Vong S, Guitian J, Holl D, Mangtani P, San S, et al. Poultry movement networks in Cambodia: implications for surveillance and control of highly pathogenic avian influenza (HPAI/H5N1). Vaccine. 2009;27:6345–52.
- Gao P, Du H, Fan L, Chen L, Liao M, Xu C, et al. Human infection with an avian-origin influenza A (H7N4) virus in Jiangsu: a potential threat to China. J Infect. 2018;77:249–57.
- Tong XC, Weng SS, Xue F, Wu X, Xu TM, Zhang WH. First human infection by a novel avian influenza A(H7N4) virus. J Infect. 2018;77:249–57.
- World Health Organization. Human infection with avian influenza A(H7N4) virus—China. Disease Outbreak News (DON) 2018 [cited 2018 Dec 17]. https://www.who.int/csr/don/22-february-2018-ah7n4-china
Suggested citation for this article: Vijaykrishna D, Deng Y-M, Grau ML, Kay M, Suttie A, Horwood PF, et al. Emergence and risk of influenza A(H7N4) virus in Cambodia. Emerg Infect Dis. 2019 Oct [date cited]. https://doi.org/10.3201/eid2510.190506
Original Publication Date: 7/16/2019