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Volume 25, Number 11—November 2019
Dispatch

High Prevalence of Mansonella ozzardi Infection in the Amazon Region, Ecuador

Manuel CalvopinaComments to Author , Carlos Chiluisa-Guacho, Alberto Toapanta, David Fonseca, and Irina Villacres
Author affiliations: Universidad de las Américas, Quito, Ecuador (M. Calvopina, A. Toapanta, D. Fonseca, I. Villacres); Instituto Nacional de Investigación en Salud Pública, Tena, Ecuador (C. Chiluisa-Guacho)

Main Article

Figure 2

Nested PCR amplification products for Mansonella ozzardi microfilariae obtained from archived human samples in the Amazon region of Ecuador. Samples were subjected to electrophoresis on a 2% agarose gel. Lanes 1 and 9, 100-bp molecular mass ladders; lanes 2, 3, 4, and 5, M. ozzardi nematode-positive samples (sample nos. 14, 53, 27, and 25, respectively) that yielded a 305-bp fragment; lane 6, Toxocara canis roundworm (610-bp fragment); lane 7, M. ozzardi nematode–negative thick blood smear; lane

Figure 2. Nested PCR amplification products for Mansonella ozzardi microfilariae obtained from archived human samples in the Amazon region of Ecuador. Samples were subjected to electrophoresis on a 2% agarose gel. Lanes 1 and 9, 100-bp molecular mass ladders; lanes 2, 3, 4, and 5, M. ozzardi nematode-positive samples (sample nos. 14, 53, 27, and 25, respectively) that yielded a 305-bp fragment; lane 6, Toxocara canis roundworm (610-bp fragment); lane 7, M. ozzardi nematode–negative thick blood smear; lane 8, negative control. These PCR results confirmed data obtained by microscopy. Morphologic characteristics of and DNA findings for the microfilariae indicated that this parasite was an M. ozzardi nematode.

Main Article

Page created: October 15, 2019
Page updated: October 15, 2019
Page reviewed: October 15, 2019
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