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Volume 25, Number 11—November 2019

Dispatch

High Prevalence of Mansonella ozzardi Infection in the Amazon Region, Ecuador

Manuel CalvopinaComments to Author , Carlos Chiluisa-Guacho, Alberto Toapanta, David Fonseca, and Irina Villacres
Author affiliations: Universidad de las Américas, Quito, Ecuador (M. Calvopina, A. Toapanta, D. Fonseca, I. Villacres); Instituto Nacional de Investigación en Salud Pública, Tena, Ecuador (C. Chiluisa-Guacho)

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Figure 2

Nested PCR amplification products for Mansonella ozzardi microfilariae obtained from archived human samples in the Amazon region of Ecuador. Samples were subjected to electrophoresis on a 2% agarose gel. Lanes 1 and 9, 100-bp molecular mass ladders; lanes 2, 3, 4, and 5, M. ozzardi nematode-positive samples (sample nos. 14, 53, 27, and 25, respectively) that yielded a 305-bp fragment; lane 6, Toxocara canis roundworm (610-bp fragment); lane 7, M. ozzardi nematode–negative thick blood smear; lane

Figure 2. Nested PCR amplification products for Mansonella ozzardi microfilariae obtained from archived human samples in the Amazon region of Ecuador. Samples were subjected to electrophoresis on a 2% agarose gel. Lanes 1 and 9, 100-bp molecular mass ladders; lanes 2, 3, 4, and 5, M. ozzardi nematode-positive samples (sample nos. 14, 53, 27, and 25, respectively) that yielded a 305-bp fragment; lane 6, Toxocara canis roundworm (610-bp fragment); lane 7, M. ozzardi nematode–negative thick blood smear; lane 8, negative control. These PCR results confirmed data obtained by microscopy. Morphologic characteristics of and DNA findings for the microfilariae indicated that this parasite was an M. ozzardi nematode.

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Page created: October 15, 2019
Page updated: October 15, 2019
Page reviewed: October 15, 2019
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