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Volume 25, Number 11—November 2019
Research Letter

Routine Culture–Resistant Mycobacterium tuberculosis Rescue and Shell-Vial Assay, France

Mustapha Fellag, Jamal Saad, Nicholas Armstrong, Eric Chabrière, Carole Eldin, Jean-Christophe Lagier, and Michel DrancourtComments to Author 
Author affiliations: Institut Hospitalier Universitaire Méditerranée Infection, Marseille, France (M. Fellag, J. Saad); Aix-Marseille University, Marseille (M. Fellag, J. Saad, N. Armstrong, E. Chabrière, C. Eldin, J.-C. Lagier, M. Drancourt)

Main Article

Figure

Light microscopic examination of human embryonic lung cells inoculated for 28 days from a clinical sample from a 47-year-old man with Pott’s disease and systemic tuberculosis, France. A) Cytopathic effect consisting of cell lysis caused by growing Mycobaterium tuberculosis. Original magnification ×200. B) M. tuberculosis mycobacteria observed after Ziehl-Neelsen staining. Original magnification ×1,000, by oil immersion. C) Absence of any cytopathic effect in negative control cell culture. Origin

Figure. Light microscopic examination of human embryonic lung cells inoculated for 28 days from a clinical sample from a 47-year-old man with Pott’s disease and systemic tuberculosis, France. A) Cytopathic effect consisting of cell lysis caused by growing Mycobaterium tuberculosis. Original magnification ×200. B) M. tuberculosis mycobacteria observed after Ziehl-Neelsen staining. Original magnification ×1,000, by oil immersion. C) Absence of any cytopathic effect in negative control cell culture. Original magnification ×200. D) Absence of any mycobacteria in the negative control cell culture after Ziehl-Neelsen staining. Original magnification ×1,000 by oil immersion.

Main Article

Page created: October 15, 2019
Page updated: October 15, 2019
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