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Volume 25, Number 3—March 2019
Research

Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programs

Juan Domínguez1, Fermín Acosta1, Laura Pérez-Lago, Dilcia Sambrano, Victoria Batista, Carolina De La Guardia, Estefanía Abascal, Álvaro Chiner-Oms, Iñaki Comas, Prudencio González, Jaime Bravo, Pedro Del Cid, Samantha Rosas, Patricia Muñoz, Amador Goodridge2Comments to Author , and Darío García de Viedma2
Author affiliations: Instituto de Investigaciones Científicas y Servicios de Alta Tecnología, City of Knowledge, Panama (J. Domínguez, F. Acosta, D. Sambrano, V. Batista, C. De La Guardia, A. Goodridge); Instituto Conmemorativo Gorgas de Estudios de la Salud, Panama City, Panama (J. Domínguez, P. González, J. Bravo, P. Del Cid, S. Rosas); Hospital General Universitario Gregorio Marañón, Madrid, Spain (F. Acosta, L. Pérez-Lago, E. Abascal, P. Muñoz, D. García de Viedma); Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain (F. Acosta, L. Pérez-Lago, E. Abascal, P. Muñoz, D. García de Viedma); Centro Superior de investigación en Salud Pública (FISABIO)–Universitat de València, Valencia, Spain (Á. Chiner-Oms); Instituto de Biomedicina de Valencia Consejo Superior de Investigaciones Científicas, Valencia (I. Comas); Centro de Investigación Biomédica en Red en Epidemiología y Salud Pública, Madrid (I. Comas); Universidad Complutense de Madrid, Madrid (P. Muñoz); Centro de Investigación Biomédica en Red Enfermedades Respiratorias, Madrid (P. Muñoz, D. García de Viedma)

Main Article

Figure 1

PCR products for allele-specific oligonucleotide PCRs for Mycobacterium tuberculosis cluster A, cluster B, and cluster C strains on a selection of representative strains A, B, and C and a selection of non-A, non-B, and non-C controls. Testing was for a isimplified model to survey tuberculosis transmission using data from patients and controls in Panama and Colon provinces, Panama, 2015. A) 308-bp PCR product (single-nucleotide polymorphism [SNP] 2) for strain A and 400-bp and 228-bp products (SN

Figure 1. PCR products for allele-specific oligonucleotide PCRs for Mycobacterium tuberculosis cluster A, cluster B, and cluster C strains on a selection of representative strains A, B, and C and a selection of non-A, non-B, and non-C controls. Testing was for a isimplified model to survey tuberculosis transmission using data from patients and controls in Panama and Colon provinces, Panama, 2015. A) 308-bp PCR product (single-nucleotide polymorphism [SNP] 2) for strain A and 400-bp and 228-bp products (SNP1/SNP3) for non-A strain. B) 127-bp and 297-bp PCR products (SNP1/SNP4) for strain B and 209-bp and 406-bp products (SNP2/SNP3) for non-B strain. C) 307-bp and 413-bp PCR products (SNP3/SNP4) for cluster C strain and 103-bp and 207-bp products (SNP1/SNP2) for non-C strain. MW, molecular weight (100-bp DNA ladder).

Main Article

1These authors contributed equally to this article.

2These authors contributed equally to this article.

Page created: February 19, 2019
Page updated: February 19, 2019
Page reviewed: February 19, 2019
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