Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programs
Juan Domínguez
1, Fermín Acosta
1, Laura Pérez-Lago, Dilcia Sambrano, Victoria Batista, Carolina De La Guardia, Estefanía Abascal, Álvaro Chiner-Oms, Iñaki Comas, Prudencio González, Jaime Bravo, Pedro Del Cid, Samantha Rosas, Patricia Muñoz, Amador Goodridge
2 , and Darío García de Viedma
2
Author affiliations: Instituto de Investigaciones Científicas y Servicios de Alta Tecnología, City of Knowledge, Panama (J. Domínguez, F. Acosta, D. Sambrano, V. Batista, C. De La Guardia, A. Goodridge); Instituto Conmemorativo Gorgas de Estudios de la Salud, Panama City, Panama (J. Domínguez, P. González, J. Bravo, P. Del Cid, S. Rosas); Hospital General Universitario Gregorio Marañón, Madrid, Spain (F. Acosta, L. Pérez-Lago, E. Abascal, P. Muñoz, D. García de Viedma); Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain (F. Acosta, L. Pérez-Lago, E. Abascal, P. Muñoz, D. García de Viedma); Centro Superior de investigación en Salud Pública (FISABIO)–Universitat de València, Valencia, Spain (Á. Chiner-Oms); Instituto de Biomedicina de Valencia Consejo Superior de Investigaciones Científicas, Valencia (I. Comas); Centro de Investigación Biomédica en Red en Epidemiología y Salud Pública, Madrid (I. Comas); Universidad Complutense de Madrid, Madrid (P. Muñoz); Centro de Investigación Biomédica en Red Enfermedades Respiratorias, Madrid (P. Muñoz, D. García de Viedma)
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Figure 2
Figure 2. Networks of relationships based on whole-genome sequencing data of prevalent clusters (A, B) and active transmission cluster (C) in testing of a simplified model to survey tuberculosis transmission using data from patients and controls in Panama and Colon provinces, Panama, 2015. Each dot corresponds to a single-nucleotide polymorphism (SNP). In (A), when 2 isolates are included in the same box, they showed no SNPs between them; the isolates within boxes with different colors show mycobacterial interspersed repetitive units–variable number of tandem repeats patterns with single-locus variations between them. Mv, median vector corresponding to nonsampled nodes; REF, reference; *Isolates from 2015 used to design the allele-specific oligonucleotide–PCRs; †strain identified in an additional analysis in Madrid (out of the prospective study applying the strain specific PCRs in Panamá).
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