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Volume 25, Number 4—April 2019
Research Letter

Malignant Aspergillus flavus Otitis Externa with Jugular Thrombosis

Maxime Moniot, Marion Montava, Stéphane Ranque, Ugo Scemama, Carole CassagneComments to Author , and Varoquaux Arthur
Author affiliations: Aix-Marseille University, Marseille, France (M. Moniot, S. Ranque, C. Cassagne); La Conception University Hospital, Marseille (M. Montava, U. Scemama, V. Arthur)

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Abstract

We report a case of malignant otitis externa with jugular vein thrombosis caused by Aspergillus flavus. Magnetic resonance imaging revealed an unusual ink smudge pattern deep in a cervical abscess. The pattern was consistent with mycetoma and may be important for diagnosing these life-threatening infections.

A 73-year-old male patient sought care from the otorhinolaryngology department at University Hospital, Marseille, France. He had a 5-month history of malignant otitis externa (MOE), which was worsening despite 4 months of treatment with intravenous ceftazidime, oral ciprofloxacin, and topical neomycin, polymyxin B, dexamethasone, and thiomersal combination. The patient had a history of high blood pressure, treated with perindopril and nicardipine, and diabetes mellitus, inadequately controlled (hemoglobin A1c 7.7%) with metformin and sitagliptin.

Figure

Thumbnail of Magnetic resonance imaging (MRI) of a patient with malignant otitis externa, France. Cross-sectional imaging demonstrates a central skull base osteomyelitis in patient’s temporal bone. A) T1-weighted imaging; B, E) 3-dimensional T1-weighted imaging with gadolinium enhancement and fat saturation; C, F, G) T2-weighted imaging; and CT with iodine enhancement (D). Single asterisks (*) indicate jugular bulb thrombosis (panels B, D); double asterisks (**) indicate deep-spaces cellulitis (

Figure. Magnetic resonance imaging (MRI) of a patient with malignant otitis externa, France. Cross-sectional imaging demonstrates a central skull base osteomyelitis in patient’s temporal bone. A) T1-weighted imaging; B, E) 3-dimensional T1-weighted...

The patient was admitted, and otoscopic examination found otorrhea, inflammation, and stenosis of the right external auditory canal; we could not see the tympanic membrane. Examination of the cranial nerve was normal. Pure-tone audiogram showed a right mixed hearing loss with air-bone gap at 15 dB and symmetric bone curve by presbycusis. Laboratory testing showed elevated erythrocyte sedimentation level (42 mm at 1 h, 82 mm at 2 h) and leukocytosis (11 g/L); C-reactive protein results were within reference range. A computed tomography (CT) scan of the head showed thickening of the ear skin; focal tympanal bone osteolysis; partial right mastoid air cells and middle-ear cavity opacification; and osteolysis of the occipital, styloid, and mastoid bones consistent with MOE (Appendix Figure). Magnetic resonance imaging (MRI) with contrast media confirmed skull base osteomyelitis, evidenced by bone lysis and marrow enhancement of the clivus (Figure, panels A–C). Both MRI and CT showed a right jugular vein thrombosis and cellulitis and abscess in the carotid and perivertebral spaces. Abscess content had an unusual aspect: T2-weighted imaging signal void foci surrounded by a hypersignal rim.

We treated the right jugular vein thrombosis with enoxaparin. The patient underwent surgical debridement with facial nerve monitoring; we collected transmastoid biopsy samples and pus for microbiological analysis and inserted a transtympanic aerator. Direct microscopic examination of the samples showed hyaline septate hyphae consistent with hyalohyphomycosis. Biopsy samples grew 2 bacteria, Corynebacterium striatum and Enterococcus faecalis, and 1 filamentous fungus, Aspergillus flavus, that we identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (Microflex LT, https://www.bruker.com) against an in-house database described by Normand et al. (1). Etest antifungal susceptibility testing (bioMérieux, https://www.biomerieux.com) showed that the A. flavus strain was sensitive to voriconazole (MIC 0.380 mg/L) and resistant to amphotericin B (MIC 12 mg/L). We stopped administration of auricular drops, continued intravenous ceftazidime (1.5 g/d) and oral ciprofloxacin (1.5 g/d), and started voriconazole therapy (6 mg/kg/12 h intravenously, followed by 400 mg/d orally). Otalgia, otorrhea, and inflammatory external auditory canal symptoms were relieved, and the patient recovered after 6 weeks. No further follow-up was available.

Fungi cause ≈10% of MOE (2). The 3 leading species, by decreasing frequency, are A. fumigatus, A. flavus, and A. niger (3). A. flavus is more frequently involved in MOE than is A. niger (3,4).

Jugular vein thrombosis (JVT) was previously reported in MOE (5) and other conditions such as Lemierre syndrome, invasive fungal infection, or any inflammatory process including otitis media. Various pathogens can cause JVT, especially Fusobacterium necrophorum and zygomycetes. Data on JVT in MOE are scarce; we could find no previously reported case of JVT related to A. flavus MOE. Postcontrast CT with soft tissue algorithm is considered the first-line imaging modality to diagnose JVT (5). In our case, both CT and MRI confirmed the diagnosis.

Osteomyelitis and abscess showed on MRI but were hardly visible on CT (Appendix Figure). High-signal T2-weighted imaging is typical in purulent content of abscesses (6). In contrast, this case exhibited an unusual lack of T2-weighted imaging signal. This characteristic pattern is known in various mycetoma locations as paranasal fungus balls (7) or maduromycosis, for which the T2-weighted imaging dot-in-circle sign is specific (8). Most authors explain the signal void as a magnetic susceptibility behavior on T2-weighted imaging resulting from accumulation of iron and other magnetic atoms (9). This case introduced a new T2-weighted imaging signal void pattern we refer to as an “ink smudge” appearance. A bone sequestrum is a differential diagnosis, yet this lesion lacked calcification in CT. We hypothesize that the ink-smudge sign we identified could be specific to fungal infection. This report should prompt careful assessment by MRI of deep-space abscess in patients with MOE.

The standard treatment for fungal MOE is a combination of surgical debridement, systemic antifungal therapy, and control of concurrent conditions. There is no consensus for the duration of the antifungal treatment; patients usually receive 6–8 of weeks antifungal therapy, more if clinical examination or imaging follow-up supports extending treatment (10).

We highlight the potential use of an MRI ink-smudge pattern to identify fungal infection in MOE. Furthermore, because we saw JVT on both postcontrast CT and MRI scans, our findings and these images may be crucial for improving patient prognosis through timely and adequate treatment.

Dr. Moniot is a resident at the University Hospital Institute Méditerranée Infection, Marseille, France. His research interests include identification and diagnosis of fungal pathogens and investigation of fungal epidemiology.

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References

  1. Normand  A-C, Cassagne  C, Gautier  M, Becker  P, Ranque  S, Hendrickx  M, et al. Decision criteria for MALDI-TOF MS-based identification of filamentous fungi using commercial and in-house reference databases. BMC Microbiol. 2017;17:25. DOIPubMed
  2. Bhandary  S, Karki  P, Sinha  BK. Malignant otitis externa: a review. Pac Health Dialog. 2002;9:647.PubMed
  3. Parize  P, Chandesris  M-O, Lanternier  F, Poirée  S, Viard  J-P, Bienvenu  B, et al. Antifungal therapy of Aspergillus invasive otitis externa: efficacy of voriconazole and review. Antimicrob Agents Chemother. 2009;53:104853. DOIPubMed
  4. Gabrielli  E, Fothergill  AW, Brescini  L, Sutton  DA, Marchionni  E, Orsetti  E, et al. Osteomyelitis caused by Aspergillus species: a review of 310 reported cases. Clin Microbiol Infect. 2014;20:55965. DOIPubMed
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  6. Matt  BH, Lusk  RP. Delineation of a deep neck abscess with magnetic resonance imaging. Ann Otol Rhinol Laryngol. 1987;96:6157. DOIPubMed
  7. Aribandi  M, McCoy  VA, Bazan  C III. Imaging features of invasive and noninvasive fungal sinusitis: a review. Radiographics. 2007;27:128396. DOIPubMed
  8. Jain  V, Makwana  GE, Bahri  N, Mathur  MK. The “dot in circle” sign on MRI in maduramycosis: a characteristic finding. J Clin Imaging Sci. 2012;2:6666. DOIPubMed
  9. Yamada  K, Zoarski  GH, Rothman  MI, Zagardo  MT, Nishimura  T, Sun  CCJ. An intracranial aspergilloma with low signal on T2-weighted images corresponding to iron accumulation. Neuroradiology. 2001;43:55961. DOIPubMed
  10. Stodulski  D, Kowalska  B, Stankiewicz  C. Otogenic skull base osteomyelitis caused by invasive fungal infection. Case report and literature review. Eur Arch Otorhinolaryngol. 2006;263:10706. DOIPubMed

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Cite This Article

DOI: 10.3201/eid2504.180710

Original Publication Date: 2/28/2019

Table of Contents – Volume 25, Number 4—April 2019

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Carole Cassagne, IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France

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Page created: March 17, 2019
Page updated: March 17, 2019
Page reviewed: March 17, 2019
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