Volume 25, Number 5—May 2019
Dispatch
Rickettsia japonica and Novel Rickettsia Species in Ticks, China
Xiang-Rong Qin, Hui-Ju Han, Fu-Jun Han, Fu-Ming Zhao, Zhen-Tang Zhang, Zai-Feng Xue, Dong-Qiang Ma, Rui Qi, Min Zhao, Li-Jun Wang, Li Zhao, Hao Yu, Jian-Wei Liu
, and Xue-Jie Yu
, and Xue-Jie Yu
Table
Primer sequences and PCR conditions used in study of Rickettsia species, China
| Target gene | Primer name | Sequence, 5′ → 3′ | Amplicon size, bp | Annealing temp, °C | Reference |
|---|---|---|---|---|---|
| rrs | S1 | TGATCCTGGCTCAGAACGAAC | 1,486 | 55 | (8) |
| S2 | TAAGGAGGTAATCCAGCCGC | ||||
| S3 | AACACATGCAAGTCGRACGG | 1,371 | 55 | ||
| S4 |
GGCTGCCTCTTGCGTTAGCT |
||||
| gltA | gltA1 | GATTGCTTTACTTACGACCC | 1,087 | 52 | (9) |
| gltA2 | TGCATTTCTTTCCATTGTGC | ||||
| gltA3 | TATAGACGGTGATAAAGGAATC | 667 | 53 | ||
| gltA4 |
CAGAACTACCGATTTCTTTAAGC |
||||
| ompB | B1 | ATATGCAGGTATCGGTACT | 1,355 | 56 | (9) |
| B2 | CCATATACCGTAAGCTACAT | ||||
| B3 | GCAGGTATCGGTACTATAAAC | 843 | 56 | ||
| B4 |
AATTTACGAAACGATTACTTCCGG |
||||
| ompB | Cand-1 | CCGGACTTTGCGGTGTAGAT | 1,136 | 52 | This study |
| Cand-2 | AAAGCCAGAAGGTGAGGCTG | ||||
| Cand-3 | ACCGCACTTGTATCGGTAGT | 874 | 50 | ||
| Cand-4 |
AAGCAGGTGGTGTAGTCGGA |
||||
| ompA | Rr190.70p | ATGGCGAATATTTCTCCAAAA | 631 | 50 | (10) |
| Rr190.701n |
GTTCCGTTAATGGCAGCATCT |
||||
| Tick mitochondrial 16S RNA | Forward | AGTATTTTGACTATACAAAGGTATTG | 408 | 55 | (7) |
| Reverse | GTAGGATTTTAAAAGTTGAACAAACTT |
Page created: April 17, 2019
Page updated: April 17, 2019
Page reviewed: April 17, 2019
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