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Volume 25, Number 5—May 2019
Research Letter

Mixed Mycobacterium tuberculosis Lineage Infection in 2 Elephants, Nepal

Sarad Paudel1, Chie Nakajima1, Susan K. Mikota, Kamal P. Gairhe, Bhagwan Maharjan, Suraj Subedi, Ajay Poudel, Mariko Sashika, Michito Shimozuru, Yasuhiko Suzuki, and Toshio TsubotaComments to Author 
Author affiliations: Hokkaido University, Sapporo, Japan (S. Paudel, C. Nakajima, M. Sashika, M. Shimozuru, Y. Suzuki, T. Tsubota); Elephant Care International, Hohenwald, Tennessee, USA (S.K. Mikota); Department of National Parks and Wildlife Conservation, Kathmandu, Nepal (K.P. Gairhe); German Nepal Tuberculosis Project, Kathmandu (B. Maharjan); National Trust for Nature Conservation, Lalitpur, Nepal (S. Subedi); Chitwan Medical College, Chitwan, Nepal (A. Poudel)

Main Article

Table

Genotypic characteristics of Mycobacterium tuberculosis isolates from 2 elephants, Nepal*

Source Spoligotype† binary code SIT Clade gyrA‡
Elephant A 1110000111111111111111001111000010111111111 New§ New§ T231C
Elephant B 0000000000000000000000001111000010111111111 1365 EAI5 T231C

*EAI5, East African–Indian 5; SIT, spoligo-international type.
†Spoligotype was determined as previously described by Brudey et al. (6).
‡Mutation in a partial sequence of gyrA. The gyrA sequence of both elephant isolates had a synonymous single nucleotide polymorphism from T to C at position 231.
§Not found in the international spoligotyping database (SpolDB4).

Main Article

1These first authors contributed equally to this article.

Page created: April 18, 2019
Page updated: April 18, 2019
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