Molecular Evidence of Human Monkeypox Virus Infection, Sierra Leone
, Jingdong Song1
, Li Zhao1
, Yi Zhang, Lianxu Xia, Lingwei Zhu, Idrissa Laybohr Kamara, Jiao Ren, Wenling Wang, Houwen Tian, Guizhen Wu, and Wenjie Tan
Author affiliations: China Center for Disease Control and Prevention, Beijing, China (F. Ye, J. Song, L. Zhao, Y. Zhang, J. Ren, W. Wang, H. Tian, G. Wu, W. Tan); Sierra Leone–China Friendship Biological Safety Laboratory, Freetown, Sierra Leone (F. Ye, J. Song, Y. Zhang, L. Xia, L. Zhu, I.L. Kamara)
Figure. Phylogenetic analysis and molecular signatures of monkeypox virus (MPXV) Sierra Leone 2017 and other collected MPXV isolates. A) Schematic representation of the MPXV Sierra Leone 2017 genomic fragment by reference to genomic data on MPXV Sierra Leone 1970. MPXV Sierra Leone 2017 contains 3 parts: an unknown region, genes encoding dUTPase, and genes encoding partial kelch-like protein. *, binding position of primers used for real-time PCR detection. Bottom panel displays genes described. Arrows indicate direction of transcription. B) Phylogenetic relationships between genomic fragments of MPXV collected in Sierra Leone and other orthopoxviruses. Neighbor-joining phylograms constructed by using MEGA6 (https://www.megasoftware.net) and the maximum-likelihood method. Scale bar indicates nucleotide substitutions per site.
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Page updated: May 20, 2019
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