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Volume 25, Number 6—June 2019
Research Letter

Reemergence of Classical Swine Fever, Japan, 2018

Alexander Postel1, Tatsuya Nishi1, Ken-ichiro Kameyama, Denise Meyer, Oliver Suckstorff, and Paul BecherComments to Author 
Author affiliations: University of Veterinary Medicine, Hannover, Germany (A. Postel, D. Meyer, O. Suckstorff, P. Becher); National Agriculture and Food Research Organization, Tokyo, Japan (T. Nishi, K. Kameyama, K. Fukai)

Main Article

Figure

Phylogenetic tree displaying the genetic relatedness of the classical swine fever virus (CSFV) isolate obtained from the 2018 classical swine fever (CSF) outbreak in Japan to other CSFV isolates. Phylogenetic analyses were performed by using the neighbor-joining method with complete E2 (1,119 nt) sequences, including 1,000 iterations for bootstrap analysis, and were generated by the genetic typing module of the CSF database at the European Union and OIE Reference Laboratory for Classical Swine F

Figure. Phylogenetic tree displaying the genetic relatedness of the classical swine fever virus (CSFV) isolate obtained from the 2018 classical swine fever (CSF) outbreak in Japan to other CSFV isolates. Phylogenetic analyses were performed by using the neighbor-joining method with complete E2 (1,119 nt) sequences, including 1,000 iterations for bootstrap analysis, and were generated by the genetic typing module of the CSF database at the European Union and OIE Reference Laboratory for Classical Swine Fever (6). Only bootstrap values >70% are indicated. For each isolate, country and year are given, along with catalog number of the CSF database and the GenBank accession number in parentheses. The arrow indicates the sequence of the virus isolate obtained from a domestic pig during the 2018 CSF outbreak in Japan (Japan/2018) (GenBank accession no. LC425432). Boldface indicates additional newly generated sequences (GenBank accession nos. MK026451–65). Two ancient sequences from Japan (CSF0742, CSF0743) were identified as belonging to genotype 2 with no clear affiliation to any of the 3 established subgenotypes 2.1, 2.2, and 2.3 (indicated as genotype 2, unclassified). Asterisks indicate 5 E2 sequences belonging to a group of sequences that are most closely related to the E2 sequence of the CSFV isolate Japan/2018. Scale bar indicates nucleotide substitutions per site.

Main Article

References
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1These authors contributed equally to this article.

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