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Volume 25, Number 8—August 2019
Dispatch

Lethal Encephalitis in Seals with Japanese Encephalitis Virus Infection, China, 2017

Xiangdong Li1, Mingming Qiao1, Xiaoyu Deng, Xi Chen, Shengyong Sun, Qian Zhang, Wenjie Zhang, Feifei Tan, Zhe Sun, Xizhao Chen, Ming SunComments to Author , and Kegong Tian2Comments to Author 
Author affiliations: National Research Center for Veterinary Medicine, Luoyang, China (X. Li, F. Tan, Z. Sun, K. Tian); Beijing Anheal Laboratories Co. Ltd, Beijing, China (M. Qiao, X. Deng, Xi Chen, S. Sun, Q. Zhang, W. Zhang, Xizhao Chen, M. Sun); Henan Agricultural University, Zhengzhou, China (K. Tian)

Main Article

Table

Primers used to amplify the full-length genome of JEV isolate Seal-Anheal-2017 obtained from a seal with lethal encephalitis, China, 2017*

Primer pair no. Primer name Primer binding site Length of fragment amplified, bp
1
 JEV-1F 1–26 879
JEV-1R
 878–855
2
 JEV-2F 805–823 833
JEV-2R
 1,637–1,615
3
 JEV-3F 1,559–1,580 703
JEV-3R
 2,261–2,242
4
 JEV-4F 2,151–2,170 827
JEV-4R
 2,977–2,959
5
 JEV-5F 2,898–2,917 779
JEV-5R
 3,676–3,658
6
 JEV-6F 3,582–3,599 740
JEV-6R
 4,321–4,300
7
 JEV-7F 4,204–4,224 919
JEV-7R
 5,122–5,104
8
 JEV-8F 5,005–5,024 934
JEV-8R
 5,938–5,918
9
 JEV-9F 5,841–5,858 939
JEV-9R
 6,779–6,757
10
 JEV-10F 6,676–6,697 880
JEV-10R
 7,555–7,537
11
 JEV-11F 7,431–7,451 817
JEV-11R
 8,247–8,228
12
 JEV-12F 8,121–8,139 875
JEV-12R
 8,995–8,974
13
 JEV-13F 8,924–8,946 866
JEV-13R
 9,789–9,771
14
 JEV-14F 9,653–9,675 827
JEV-14R
 10,479–10,460
15 JEV-15F 10,217–10,236 769

*JEV, Japanese encephalitis virus.

Main Article

1These authors contributed equally to this article.

2These senior authors contributed equally to this article.

Page created: July 16, 2019
Page updated: July 16, 2019
Page reviewed: July 16, 2019
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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