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Volume 25, Number 8—August 2019
Research Letter

Intrafamily Transmission of Monkeypox Virus, Central African Republic, 2018

Camille Besombes, Ella Gonofio, Xavier Konamna, Benjamin Selekon, Antoine Gessain, Nicolas Berthet, Jean-Claude Manuguerra, Arnaud Fontanet, and Emmanuel NakounéComments to Author 
Author affiliations: Institut Pasteur, Paris, France (C. Besombes, A. Gessain, N. Berthet, J.-C. Manuguerra, A. Fontanet); Institut Pasteur de Bangui, Bangui, Central African Republic (E. Gonofio, X. Konamna, B. Selekon, E. Nakouné); Centre National de la Recherche Scientifique, Paris (A. Gessain, N. Berthet); Unité Pasteur-CNAM Risques Infectieux et Emergents, Conservatoire National des Arts et Métiers, Paris (A. Fontanet)

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Abstract

Monkeypox is a rare viral zoonotic disease; primary infections are reported from remote forest areas of Central and West Africa. We report an investigation of a monkeypox outbreak in Lobaye, southwest Central African Republic, in October 2018.

Monkeypox, a zoonotic disease caused by an Orthopoxvirus, has clinical signs and symptoms in humans similar to smallpox and a case-fatality rate of 10% (1). The specific reservoir species for monkeypox virus remains, to a large extent, unidentified (2). Spillover events of monkeypox have been reported in remote forest areas of Central and West Africa. After zoonotic infection, the virus can be transmitted from person to person (1).

To date, human monkeypox outbreaks in the Central African Republic (CAR) have been small: ≈10 cases, restricted to a family or village. Primary infection in these outbreaks occurred from contact with wild fauna, with secondary transmission among close contacts in the community (3,4) and limited nosocomial transmission (5). Since 2000, the Virology Laboratory of the Institut Pasteur de Bangui (IP Bangui), a regional reference center for monkeypox, has reported 20 monkeypox outbreaks across several regions of CAR, totaling ≈100 cases, particularly in the region of Lobaye (3,4). In 2018 alone, IP Bangui investigated 6 different outbreaks in CAR, indicating a possible increase in frequency of outbreaks (6,7).

On September 27, 2018, a healthcare worker from Zomea Kaka healthcare center in Lobaye reported to IP Bangui about 3 cases of suspected monkeypox in an Aka Pygmy family. A 25-year-old female sought care at the health center, 10 km from her village, for maculopapular rash and lesions. She was afebrile. Her signs and symptoms indicated of resolving late stage monkeypox infection. She was accompanied by her 2 daughters, 5 months and 4 years of age, both showing typical symptoms of active monkeypox infection, notably maculopapular rash on the palms of their hands and soles of their feet (Appendix Figure). Blood or pus samples taken from the 3 patients were confirmed positive for monkeypox infection by PCR on September 29 (8) (Appendix).

On October 5, IP Bangui carried out an investigation among contacts of the index case-patient, in collaboration with the Ministry of Health and the World Health Organization CAR Country Office. The index case-patient reported butchering 3 small mammals known in local Aka language as Yabo (African civet, Civettictis civetta), Gbè (Emin’s pouched rat, Cricetomys emini), and Sende (African rope squirrel, Funesciurus anerythrus). She butchered 1 of each in a forested area 2 weeks before the onset of rash.

During October 6–10, two additional family contacts from the village, the index case-patient’s 2 sisters, 7 and 16 years of age, reported symptoms consistent with monkeypox infection. Healthcare workers collected blood or pus samples from the patients, and IP Bangui confirmed monkeypox infection by PCR. On October 26, monkeypox infection was confirmed in another family contact, the index case-patient’s 33-year-old sister-in-law. The dates of the onset of symptoms suggest 3 waves of intrafamilial transmission (Table) (9).

IP Bangui conducted further investigations by using Orthopoxvirus serologic assays (Appendix) on blood samples collected from 2 healthcare worker contacts on October 5 and from 31 village contacts on October 25. Results revealed evidence of Orthopoxvirus serologic response in the index case-patient’s mother; 2 healthcare workers who had cared for the index case-patient; and the index case-patient’s brother, who brought her the wild animals (Table).

Serologic evidence of possible monkeypox infection can indicate prior exposure to the virus or, among persons >38 years of age, immunization against smallpox, and might explain the restricted size of the outbreak in the village. However, smallpox vaccination campaigns with a live-attenuated vaccinia virus ended in 1979 in CAR. Consequently, an increasingly larger proportion of the population is immunologically naive to Orthopoxvirus infection.

This investigation identified 5 clinical cases of secondary monkeypox infection spread over 3 waves of intrafamilial infection, originating from an index case-patient with primary infection possibly attributable to contact with wild fauna. The prompt declaration and isolation of suspected cases, as well as possible naturally acquired immunity or persistence of vaccine-derived immunity within the community, likely contributed to the restricted extent of secondary transmission. Further studies are needed to clarify risk factors for primary and secondary monkeypox transmission.

Positive serologic findings in healthcare workers during this investigation also highlight the limited infection prevention and control resources, such as isolation rooms, gowns, gloves, N95 respirators, and goggles, to protect healthcare workers responding to outbreaks in CAR. For communities located in remote forest areas in which zoonotic spillover and secondary transmission are thought to occur regularly, health center capacity and resources need to be strengthened. Health centers urgently need training on case recognition for healthcare workers, access to diagnostic capacities, and appropriate infection prevention and control measures to reduce the possibility of secondary transmission in these areas (10).

Dr. Besombes is an infectious and tropical disease clinician who works as a researcher in the Emerging Diseases Epidemiology Unit at Institut Pasteur, Paris, France. Her primary research interests include tropical diseases, specifically zoonotic and vectorborne diseases, and hepatitis Delta virus infection.

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Acknowledgment

The authors thank Romain Duda for his assistance with identification of the animal species in Aka language. We also acknowledge Rebecca Grant for her relevant suggestion and her kind participation in the formatting of this research letter.

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References

  1. Durski  KN, McCollum  AM, Nakazawa  Y, Petersen  BW, Reynolds  MG, Briand  S, et al. Emergence of Monkeypox - West and Central Africa, 1970-2017. MMWR Morb Mortal Wkly Rep. 2018;67:30610. DOIPubMed
  2. Sklenovská  N, Van Ranst  M. Emergence of monkeypox as the most important orthopoxvirus infection in humans. Front Public Health. 2018;6:241. DOIPubMed
  3. Berthet  N, Nakouné  E, Whist  E, Selekon  B, Burguière  AM, Manuguerra  JC, et al. Maculopapular lesions in the Central African Republic. Lancet. 2011;378:1354. DOIPubMed
  4. Kalthan  E, Tenguere  J, Ndjapou  SG, Koyazengbe  TA, Mbomba  J, Marada  RM, et al. Investigation of an outbreak of monkeypox in an area occupied by armed groups, Central African Republic. Med Mal Infect. 2018;48:2638. DOIPubMed
  5. Nakoune  E, Lampaert  E, Ndjapou  SG, Janssens  C, Zuniga  I, Van Herp  M, et al. A nosocomial outbreak of human monkeypox in the Central African Republic. Open Forum Infect Dis. 2017;4:ofx168.
  6. ProMED-mail. Monkeypox—Africa (12): Central African Republic. 2018 Jul 30 [cited 2018 Oct 24]. https://www.promedmail.org, archive no. 20180730.5936829.
  7. ProMED-mail. Monkeypox—Africa (05): Central African Republic (Haute-Kotto). 2018 Apr 4 [cited 2019 Jan 7]. https://www.promedmail.org, archive no. 20180403.5726159.
  8. Li  Y, Zhao  H, Wilkins  K, Hughes  C, Damon  IK. Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA. J Virol Methods. 2010;169:2237. DOIPubMed
  9. Di Giulio  DB, Eckburg  PB. Human monkeypox: an emerging zoonosis. Lancet Infect Dis. 2004;4:1525. DOIPubMed
  10. Munster  VJ, Bausch  DG, de Wit  E, Fischer  R, Kobinger  G, Muñoz-Fontela  C, et al. Outbreaks in a rapidly changing Central Africa—lessons from Ebola. N Engl J Med. 2018;379:1198201. DOIPubMed

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Cite This Article

DOI: 10.3201/eid2508.190112

Original Publication Date: 6/19/2019

Table of Contents – Volume 25, Number 8—August 2019

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Emmanuel Nakouné, Arboviruses, Viral Haemorragic Viruses, Emerging Viruses and Zoonosis Unit, Institut Pasteur de Bangui, Bangui, Central African Republic

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Page created: July 17, 2019
Page updated: July 17, 2019
Page reviewed: July 17, 2019
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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