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Volume 26, Number 1—January 2020
Research

Preclinical Detection of Prions in Blood of Nonhuman Primates Infected with Variant Creutzfeldt-Jakob Disease

Luis Concha-Marambio1, Marcelo A. Chacon, and Claudio SotoComments to Author 
Author affiliations: University of Texas, Houston, Texas, USA (L. Concha-Marambio, M.A. Chacon, C. Soto); Universidad de los Andes, Santiago, Chile (L. Concha-Marambio, C. Soto)

Main Article

Figure 2

Detection of macaque-adapted vCJD prions in blood and blood fractions of macaques collected at the final bleed. WB, RBC, BC, and PL collected at the terminal bleed of 3 macaques infected with macaque-adapted vCJD and 1 noninfected control were processed and analyzed by 4 PMCA rounds. Dilutions of vCJD BH of 10−5 (−5) and 10−9 (−9) were analyzed as positive controls; an unseeded reaction (−) was used as a negative control. After completion of the 4 rounds of PMCA, samples were digested with 50 μg

Figure 2. Detection of macaque-adapted vCJD prions in blood and blood fractions of macaques collected at the final bleed. WB, RBC, BC, and PL collected at the terminal bleed of 3 macaques infected with macaque-adapted vCJD and 1 noninfected control were processed and analyzed by 4 PMCA rounds. Dilutions of vCJD BH of 10−5 (−5) and 10−9 (−9) were analyzed as positive controls; an unseeded reaction (−) was used as a negative control. After completion of the 4 rounds of PMCA, samples were digested with 50 μg/mL of proteinase K and analyzed by Western blot. N refers to transgenic mouse normal BH without proteinase K treatment, which was used as a migration control. BC, buffy coat; BH, brain homogenate; PL, plasma; PMCA, protein misfolding cyclic amplification; RBC, erythrocytes; vCJD, variant Creutzfeldt-Jakob disease; WB, whole blood.

Main Article

1Current affiliation: Amprion, Inc., San Diego, California, USA.

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