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Volume 26, Number 1—January 2020

Research

Preclinical Detection of Prions in Blood of Nonhuman Primates Infected with Variant Creutzfeldt-Jakob Disease

Luis Concha-Marambio1, Marcelo A. Chacon, and Claudio SotoComments to Author 
Author affiliations: University of Texas, Houston, Texas, USA (L. Concha-Marambio, M.A. Chacon, C. Soto); Universidad de los Andes, Santiago, Chile (L. Concha-Marambio, C. Soto)

Main Article

Figure 5

Preclinical detection of macaque-adapted vCJD prions in BC of peripherally infected macaques. A total of 140 deidentified samples (500 μL each) were sarkosyl precipitated and analyzed by 5 rounds of PMCA. After amplification, samples from the fourth and fifth rounds were digested with 50 μg/mL of PK and analyzed by Western blot. N refers to transgenic mouse normal BH without proteinase K treatment, which was used as a migration control. BH, brain homogenate; m-vCJD, macaque-adapted vCJD PMCA; pr

Figure 5. Preclinical detection of macaque-adapted vCJD prions in BC of peripherally infected macaques. A total of 140 deidentified samples (500 μL each) were sarkosyl precipitated and analyzed by 5 rounds of PMCA. After amplification, samples from the fourth and fifth rounds were digested with 50 μg/mL of PK and analyzed by Western blot. N refers to transgenic mouse normal BH without proteinase K treatment, which was used as a migration control. BH, brain homogenate; m-vCJD, macaque-adapted vCJD; PMCA, protein misfolding cyclic amplification; vCJD, variant Creutzfeldt-Jakob disease.

Main Article

1Current affiliation: Amprion, Inc., San Diego, California, USA.

Page created: December 17, 2019
Page updated: December 17, 2019
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