Figure 5. Preclinical detection of macaque-adapted vCJD prions in BC of peripherally infected macaques. A total of 140 deidentified samples (500 μL each) were sarkosyl precipitated and analyzed by 5 rounds of PMCA. After amplification, samples from the fourth and fifth rounds were digested with 50 μg/mL of PK and analyzed by Western blot. N refers to transgenic mouse normal BH without proteinase K treatment, which was used as a migration control. BH, brain homogenate; m-vCJD, macaque-adapted vCJD; PMCA, protein misfolding cyclic amplification; vCJD, variant Creutzfeldt-Jakob disease.