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Volume 26, Number 10—October 2020
Dispatch

Limitations of Ribotyping as Genotyping Method for Corynebacterium ulcerans

Tsuyoshi Sekizuka, Chihiro Katsukawa1, Makoto Kuroda, Keigo Shibayama, Ken Otsuji, Mitsumasa Saito, Akihiko Yamamoto, and Masaaki IwakiComments to Author 
Author affiliations: National Institute of Infectious Diseases, Tokyo, Japan (T. Sekizuka, M. Kuroda, K. Shibyama, A. Yamamoto, M. Iwaki); Osaka Prefectural Institute of Public Health, Osaka, Japan (C. Katsukawa); University of Occupational and Environmental Health, Kitakyushu, Japan (K. Otsuji, M. Saito)

Main Article

Table

Conserved mutation sites among strains 0102, 0211, and FH2016–1 of Corynebacterium ulcerans, Japan, 2001–2016*

Position (strain 0102) Strain 0102 Strain 0211 FH2016–1 Mutation type Locus_tag, gene ORF length, bp Product Detected mutation in each ORF Amino acid substitution Description
13,243 T TTC T Insertion CULC0102_0011 705 Putative membrane protein 293T>TTC NA Pseudogene in strain 0211
147,936 T C T SNV CULC0102_0143 873 Putative ABC transporter, substrate binding protein 476T>C Leu159Ser NA
270,551 AT A AT Deletion CULC0102_0261 3,204 Putative surface-anchored membrane protein 751AT>A NA Pseudogene in strain 0211
408,576 C T C SNV CULC0102_0389 (menD) 1,614 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase 1574C>T Ala525Val NA
561,350 A AT A Insertion CULC0102_0552 297 Hypothetical protein 218A>AT NA Pseudogene in strain 0211
820,419 G T G SNV CULC0102_0788 5,073 Putative helicase 2928G>T Lys976Asn GO term: DNA metabolic process
989,653 C T C SNV CULC0102_0951 1,143 Hypothetical protein 965G>A Gly322Asp NA
1,058,136 G A G SNV CULC0102_1010 468 Putative membrane protein 88G>A Gly30Ser NA
1,094,809 T C T SNV CULC0102_1045 1,143 Putative glutathione S-transferase 55A>G Thr19Ala NA
1,709,596 G T G SNV CULC0102_1586 (trpC2) 810 Indole-3-glycerol phosphate synthase 291C>A His97Gln NA
1,886,000 C T C SNV CULC0102_1749 (hrcA) 1,047 Heat-inducible transcription repressor 232G>A Val78Met GO term: DNA metabolic process
2,038,758 GGC...GCA (123 bp) G GGC...GCA (123 bp) Deletion Intergenic_region NA NA NA NA NA
2,104,276 G A G SNV CULC0102_1940 393 Hypothetical protein 346C>T Arg116Cys NA
2,126,945 C T C SNV CULC0102_1961 1,185 Putative secreted esterase 1072C>T Arg358Stop Pseudogene in strain 0211
2,401,441 T C T SNV CULC0102_2194 (glf) 1,197 UDP-galactopyranose mutase 80A>G Asn27Ser NA

*NA, not applicable; ORF, open reading frame; SNV, single-nucleotide variation.

Main Article

1Current affiliation: Osaka Prefecture University, Osaka, Japan.

Page created: July 28, 2020
Page updated: September 28, 2020 1:09 PM EDT
Page reviewed: September 28, 2020 1:09 PM EDT
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