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Volume 26, Number 12—December 2020

Dispatch

Shedding of Marburg Virus in Naturally Infected Egyptian Rousette Bats, South Africa, 2017

Janusz T. PawęskaComments to Author , Nadia Storm, Wanda Markotter, Nicholas Di Paola, Michael R. Wiley, Gustavo Palacios, and Petrus Jansen van Vuren
Author affiliations: National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, South Africa (J.T. Pawęska, N. Storm, P. Jansen van Vuren); Boston University, Boston, Massachusetts, USA (N. Storm); University of Pretoria, Pretoria, South Africa (J.T. Pawęska, W. Markotter); University of Nebraska Medical Center, Omaha, Nebraska, USA (N. Di Paola, M.R. Wiley, G. Palacios); US Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA (N. Di Paola, M.R. Wiley, G. Palacios); Australian Centre for Disease Preparedness, Commonwealth Scientific and Industrial Research Organisation–Health and Biosecurity, Geelong, Victoria, Australia (P. Jansen van Vuren)

Main Article

Figure 1

Midpoint-rooted, maximum-likelihood phylogeny of complete and near-complete MARV and RAVV genomes. Phylogenetic tree shows evolutionary relationships of marburgvirus detected in a rectal swab sample from a subadult Egyptian rousette female bat (Rousettus aegyptiacus) in Matlapisi Cave, Limpopo Province, South Africa, 2017 (black filled circle; GenBank accession no. MT321489), and reference viruses, including the SPU191-13 bat 2764 Mahlapitsi strain (white circle; GenBank accession no. MG725616), detected in the same cave in July 2013. Complete and near-complete genome sequences from GenBank (accession numbers indicated) were aligned with the partial MARV sequence obtained from RSA-8095bat using MUSCLE version 3.8.31 (https://www.drive5.com/muscle), and RAxML version 8.2.10 (https://cme.h-its.org/exelixis/web/software/raxml/index.html) was used to infer the best-scoring maximum-likelihood tree after 1,000 bootstrap replicates. Node values indicate the bootstrap support values. Genomes isolated from bats are shown using a bat symbol. Scale bar indicates nucleotide substitutions per site. MARV, Marburg virus; RAVV, Ravn virus.

Figure 1. Midpoint-rooted, maximum-likelihood phylogeny of complete and near-complete MARV and RAVV genomes. Phylogenetic tree shows evolutionary relationships of marburgvirus detected in a rectal swab sample from a subadult Egyptian rousette female bat (Rousettus aegyptiacus) in Matlapisi Cave, Limpopo Province, South Africa, 2017 (black filled circle; GenBank accession no. MT321489), and reference viruses, including the SPU191-13 bat 2764 Mahlapitsi strain (white circle; GenBank accession no. MG725616), detected in the same cave in July 2013. Complete and near-complete genome sequences from GenBank (accession numbers indicated) were aligned with the partial MARV sequence obtained from RSA-8095bat using MUSCLE version 3.8.31 (https://www.drive5.com/muscle), and RAxML version 8.2.10 (https://cme.h-its.org/exelixis/web/software/raxml/index.html) was used to infer the best-scoring maximum-likelihood tree after 1,000 bootstrap replicates. Node values indicate the bootstrap support values. Genomes isolated from bats are shown using a bat symbol. Scale bar indicates nucleotide substitutions per site. MARV, Marburg virus; RAVV, Ravn virus.

Main Article

Page created: August 12, 2020
Page updated: November 19, 2020
Page reviewed: November 19, 2020
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