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Volume 26, Number 2—February 2020
Research

Porcine Deltacoronavirus Infection and Transmission in Poultry, United States1

Patricia A. Boley, Moyasar A. Alhamo, Geoffrey Lossie, Kush Kumar Yadav, Marcia Vasquez-Lee, Linda J. Saif, and Scott P. KenneyComments to Author 
Author affiliations: The Ohio State University, Wooster, Ohio, USA (P.A. Boley, M.A. Alhamo, K.K. Yadav, M. Vasquez-Lee, L.J. Saif, S.P. Kenney); The Ohio State University, Columbus, Ohio, USA (G. Lossie)

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Figure 2

Detection of porcine deltacoronavirus (PDCoV) antigen in poultry by immunofluorescent (IF) staining in the intestines of poults inoculated with intestinal contents of a PDCoV infected GF pig (DC175) (6.87 log10 50% tissue culture infectious dose/mL) or a mock inoculate in a study of infection and transmission of porcine deltacoronavirus in poultry. A) PDCoV-infected pig intestine used as positive control; white arrows indicate widespread antibody staining. B) IF-stained jejunum of a poult (no. 4

Figure 2. Detection of porcine deltacoronavirus (PDCoV) antigen in poultry by immunofluorescent (IF) staining in the intestines of poults inoculated with intestinal contents of a PDCoV-infected GF pig (DC175) (6.87 log10 50% tissue culture infectious dose/mL) or a mock inoculate in a study of infection and transmission of porcine deltacoronavirus in poultry. A) PDCoV-infected pig intestine used as positive control; white arrows indicate widespread antibody staining. B) IF-stained jejunum of a poult (no. 42) at 14 dpi with no antigen-positive cells. C) IF-stained jejunum of a poult (no. 63) at 14 days postinoculation (dpi); white arrows indicate several PDCoV antigen-positive cells in the villous epithelial cells. Original magnification ×300.

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1Preliminary results of this study were presented at the Conference of Research Workers in Animal Diseases, December 1–4, 2018, Chicago, Illinois, USA.

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Page updated: January 17, 2020
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