Volume 26, Number 2—February 2020
Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness
|Aligned to||Mismatches||Indels||Contigs||Nucleotide identity, %; average-fold coverage
|Ames reference strain|
|Nanopore||677||6,411||3||99.83; 54||99.78; 192||99.80; 91|
|SRS||526||180||35||99.96; 115||99.94; 467||99.94; 220|
*Mismatches, indels, nucleotide identity, and average fold coverage for chromosomal and plasmid sequences of B. anthracis strain Ba0914 were determined on the basis of alignment with the Ames Ancestor reference strain assembly (top) or to the SRS-based Ba0914 strain assembly (bottom). The nanopore assembly was generated by using the first 120,000 live basecalled reads. Contigs, contiguous overlapping DNA segments; Indels, insertions and deletions; NA, not applicable; SRS, short-read sequencing.