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Volume 26, Number 5—May 2020
Research Letter

Diplorickettsia Bacteria in an Ixodes scapularis Tick, Vermont, USA

Carter Merenstein, Jeremy Ward, and David AllenComments to Author 
Author affiliations: Boston University School of Medicine, Boston, Massachusetts, USA (C. Merenstein); Middlebury College, Middlebury, Vermont, USA (J. Ward, D. Allen)

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Figure

Neighbor-joining phylogenetic tree of a MAFFT alignment (https://mafft.cbrc.jp/alignment/server) of the V3–V4 region of the Diplorickettsia 16S rRNA gene, including the novel amplicon sequence variant identified in Vermont, USA (bold). A total of 427 bases were aligned and 363 conserved sites were used for neighbor-joining phylogeny, with 100 bootstrap iterations. The 341F and 875R primers were used to amplify these regions (6). Default alignment parameters were used for alignment and generation

Figure. Neighbor-joining phylogenetic tree of a MAFFT alignment (https://mafft.cbrc.jp/alignment/server) of the V3–V4 region of the Diplorickettsia 16S rRNA gene, including the novel amplicon sequence variant identified in Vermont, USA (bold). A total of 427 bases were aligned and 363 conserved sites were used for neighbor-joining phylogeny, with 100 bootstrap iterations. The 341F and 875R primers were used to amplify these regions (6). Default alignment parameters were used for alignment and generation of phylogenetic tree. Numbers at nodes indicate bootstrap values after 1,000 bootstrapping iterations. GenBank accession numbers are indicated. Scale bar represents average number of substitutions per site.

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