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Volume 26, Number 6—June 2020
Research

Zoonotic Alphaviruses in Fatal and Neurologic Infections in Wildlife and Nonequine Domestic Animals, South Africa

Jumari Steyn, Isabel Fourie, Johan Steyl, June Williams, Voula Stivaktas, Elizabeth Botha, Stefanie van Niekerk, Bjorn Reininghaus, and Marietjie VenterComments to Author 
Author affiliations: University of Pretoria, Pretoria, South Africa (J. Steyn, I. Fourie, J. Steyl, J. Williams, V. Stivaktas, E. Botha, S. van Niekerk, M. Venter); Mpumalanga Veterinary Services, Middelburg, South Africa (B. Reininghaus)

Main Article

Figure 3

Phylogram of the RNA-dependent RNA polymerase gene (348-bp fragment) of alphaviruses rooted at the midpoint and created by using maximum-likelihood analysis (67 taxa, generalized time-reversible model with gamma distribution of rates across sites). Black circles indicate wildlife, domestic animals, and birds from South Africa, February 2010–September 2018, and open circles indicate previously reported virus-positive horses (11). Numbers on branches are bootstrap support values. Values are shown

Figure 3. Phylogram of the RNA-dependent RNA polymerase gene (348-bp fragment) of alphaviruses rooted at the midpoint and created by using maximum-likelihood analysis (67 taxa, generalized time-reversible model with gamma distribution of rates across sites). Black circles indicate wildlife, domestic animals, and birds from South Africa, February 2010–September 2018, and open circles indicate previously reported virus-positive horses (11). Numbers on branches are bootstrap support values. Values are shown if they are >60. Sample identification and GenBank Accession numbers: MVA51/10, MK114099; ZRU139/18, MK114091; ZRU140/18, MK114087; ZRU158/14, MK114089; ZRU160/18, MK114092, ZRU203/18_Lung, MK114094; ZRU203/18_Spleen, MK114093; ZRU204/18, MK114095; ZRU209/15, MK114096; ZRU211/15, MK114097; ZRU214/18, MK114098; ZRU54/13, MK114090; ZRU93/15, MK114088. Reference strain, name, accession number, and origin are as described by Forrester et al. (6). Scale bar indicates nucleotide substitutions per site. EEEV, Eastern equine encephalitis virus; MIDV, Middelburg virus; SFV, Semliki Forest virus; SPDV, salmon pancreas disease virus; WEEV, Western equine encephalitis virus.

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