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Volume 26, Number 6—June 2020
Dispatch

Prevalence of Escherichia albertii in Raccoons (Procyon lotor), Japan

Atsushi Hinenoya, Keigo Nagano, Sharda P. Awasthi, Noritoshi Hatanaka, and Shinji YamasakiComments to Author 
Author affiliations: Osaka Prefecture University, Osaka, Japan

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Abstract

Natural reservoirs of Escherichia albertii remain unclear. In this study, we detected E. albertii by PCR in 248 (57.7%) of 430 raccoons from Osaka, Japan, and isolated 143 E. albertii strains from the 62 PCR-positive samples. These data indicate that raccoons could be a natural reservoir of E. albertii in Japan.

Escherichia albertii is a gram-negative facultative anaerobic bacterium and an emerging human enteropathogen. This bacterium belongs to the group of attaching and effacing pathogens, which can form pedestal-structured lesions on intestinal epithelium by using an eae-encoded adhesin called intimin and a type 3 secretion system. E. albertii commonly carries cytolethal distending toxin genes; in addition, certain strains carry Shiga toxin 2 (stx2a, stx2f) genes (1), suggesting that E. albertii has a potential to cause severe diseases such as hemorrhagic colitis and hemolytic uremic syndrome in humans, similar to Shiga toxin–producing E. coli. An increase in human outbreaks and sporadic cases of E. albertii have been reported recently from several countries, including Japan (13). However, the reservoir and transmission routes of E. albertii to humans have not yet been identified. We surveyed wild raccoons (Procyon lotor) captured in Osaka, Japan, for the presence of E. albertii to determine if raccoons could be a reservoir of E. albertii in Japan.

The Study

We collected 430 rectal swabs from wild raccoons in Osaka during 2016–2017 (Appendix). To determine the presence of E. albertii, we first subjected fecal specimens to an E. albertii–specific cdt (Eacdt) gene-based PCR assay (4) after enrichment in tryptic soy broth. Of these 430 specimens, 248 (57.7%) yielded a 449-bp PCR amplicon specific for E. albertii (Table 1). By using XRM-MacConkey agar developed for the isolation of E. albertii (Appendix), we isolated and selected 143 E. albertii isolates from the 62 PCR-positive specimens (1–8 isolates/sample) with species identity confirmed by 2 different E. albertii–specific PCRs using primers targeting Eacdt (4), and yejH and yejK (5).

Figure

Thumbnail of Phylogenetic analysis of raccoon Escherichia albertii strains by pulsed-field gel electrophoresis (PFGE). XbaI-digested genomic DNA of 143 raccoon E. albertii strains isolated in this study were analyzed by PFGE. The dendrogram was constructed based on DNA fingerprints obtained (Appendix). The number in each strain name represents a specific raccoon identification number.

Figure. Phylogenetic analysis of raccoon Escherichia albertii strains by pulsed-field gel electrophoresis (PFGE). XbaI-digested genomic DNA of 143 raccoon E. albertii strains isolated in this study were analyzed by PFGE. The dendrogram...

To determine the phylogenetic relationships among the isolates, we performed pulsed-field gel electrophoresis (PFGE) using XbaI-digested genomic DNA. The 143 isolates showed 59 pulsotypes (Figure), indicating that E. albertii isolates from raccoons were genetically diverse. We obtained 2–7 E. albertii isolates, which were determined to be clonal by PFGE, from 26 of 29 raccoons. The isolates from each of 3 raccoons (R305, R318, R419) showed 2–3 different DNA fingerprints with >3 bands different from each other, indicating that multiclonal E. albertii strains coexisted in the intestine of each of these 3 raccoons (Figure). In addition, we frequently observed that the isolates from different raccoons displayed exactly the same PFGE pattern (e.g., R7, R8, and R335; Figure), although the raccoons were usually captured in different locations in Osaka.

To evaluate the human pathogenic potential of E. albertii isolated from raccoons, we selected 1 isolate from each pulsotype (n = 59) and tested for the presence of virulence determinants in clinical E. albertii isolates (Appendix). We detected the eae gene in 59 strains (100%), Eccdt-I in 5 strains (8.5%), and stx2f genes in 2 strains (3.4%). By sequencing the entire eae gene in 59 strains (Appendix), we determined the intimin subtypes to be ρ (n = 8), ι2 (n = 5), ο (n = 4), ς (n = 4), γ5 (n = 2), ξ (n = 2), α8 (n = 1), β3 (n = 1), and unknown (n = 32) (Appendix Table 3). Among the 32 unknown subtypes, 16 were grouped into the 5 subtypes (N1–N5) that were recently identified in clinical E. albertii strains from Japan (6). Two subtypes were homologous to those identified in clinical E. albertii strains 1251–6/89, 2 were homologous to strain 4281–7/89 (7), and 3 were homologous to those identified in strain 2013C-4143 (GenBank accession no. CP030787). We also identified 2 novel subtypes (UT1 and UT2; Appendix Table 3) in the remaining 9 strains; each showed <95% nt and aa identities with any known subtypes. We identified complete stx2f genes in the stx2f gene-positive strains RAC-199 and RAC-247. The culture supernatants caused Vero cell deaths, which were neutralized by anti-Stx2fA serum, indicating that both strains produced biologically active Stx2f. The toxin activity was enhanced in the presence of mitomycin C, indicating that the stx2f genes could be located on inducible prophage genomes (Table 2). The fold change of Stx2f production by mitomycin C in the strains RAC-199 and RAC-247 were comparable to that of Stx2 production in human clinical strains E. albertii AKT5 and EHEC O157:H7 Sakai. These data suggest that the E. albertii strains isolated from raccoons have a potential to cause serious human diseases.

Conclusions

E. albertii is known to be an emerging zoonotic pathogen and has been isolated from various animals, such as pigs, cats, and birds (6,8,9). Although much effort has been devoted to identify the natural reservoir, E. albertii was not detected in vertebrate animals such as fish (n = 138), amphibians (n = 106), reptiles (n = 447), and mammals (n = 1,063) (3) but was found in 1.4% (9/634) of birds in Australia and 0.9% (9/1,204) of birds in Korea. Thus, the natural reservoir of E. albertii is still unclear; this information would be essential to determine transmission dynamics and prevent E. albertii infections. Given that patients in clinical outbreaks in Japan might be infected thorough waters (spring and well waters) or vegetables, but not meats (3), the natural reservoir of E. albertii might not be major food animals (e.g., cattle and chickens, the reservoirs for Shiga toxin–producing E. coli and Campylobacter jejuni, respectively). Another possibility is wild animals, which may contaminate environmental water and vegetables. Among the wild animals, the raccoon is a synanthropic animal with the ability to reside in a wide range of habitats, including agricultural, forested, and urban areas. Raccoons are omnivorous and forage within vegetable fields. They also prefer riparian environments. Furthermore, raccoons are known to carry various pathogenic microorganisms (1012). Thus, they can contaminate vegetables and waters with pathogens, possibly including E. albertii, leading to human infections. Therefore, we performed a survey targeting raccoons and found that E. albertii was highly prevalent (248/430; 57.7%) in wild raccoons in Japan, indicating that carriage of E. albertii by raccoons is not incidental. The E. albertii strains isolated from raccoons also possessed virulence determinants (eae, Eacdt, Eccdt-I, or stx2f) present in human clinical strains. Almost all the intimin subtypes of the raccoon strains were those identified in human clinical E. albertii strains. Two strains produced functional Stx2f, which may have a potential to cause severe diseases in humans. Taken together, these data suggested that raccoons constitute a major reservoir of E. albertii and could be a source of human infection in Japan.

Raccoons originated from North America and were introduced as pets or game animals into other countries, including Japan and countries in Europe. Some of these have escaped and settled in the wild. The number of raccoons has increased because of their adaptability to various environments, omnivorous feeding habits, high reproductive potential, and lack of predators in the environment (13).

In addition to Japan, E. albertii has been clinically isolated in other countries where raccoons reside (9,14,15). Interactions between raccoons and other animals, such as wild mice and wild boars, can also be possible. Therefore, further epidemiologic studies to survey raccoons and other wild animals in Osaka, other areas of Japan, and other countries are highly warranted to evaluate the significance of raccoons as a natural reservoir of E. albertii.

Dr. Hinenoya is an associate professor at the Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan. His research interests include molecular epidemiology and pathogenicity of pathogenic bacteria, especially zoonotic pathogens.

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Acknowledgments

We thank the Osaka Livestock Hygiene Service Center for providing raccoon fecal samples.

This work was supported in part by Japan Society for the Promotion of Science KAKENHI (grant no. 17H04651) and AMED (grant no. JP16jk021007).

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References

  1. Bhatt  S, Egan  M, Critelli  B, Kouse  A, Kalman  D, Upreti  C. The evasive enemy: insights into the virulence and epidemiology of the emerging attaching and effacing pathogen Escherichia albertii. Infect Immun. 2018;87:e00254-18. DOIPubMed
  2. National Institute of Infectious Diseases and Tuberculosis and Infectious Diseases Control Division, Ministry of Health, Labour and Welfare. Food poisoning due to Escherichia albertii, May 2017—Utsunomiya city in Japan [in Japanese]. Infect Agents Surveillance Rep. 2017;38:1756.
  3. Ooka  T. Emerging enteropathogen, Escherichia albertii [in Japanese]. Jpn J Food Microbiol. 2017;34:1517. DOI
  4. Hinenoya  A, Ichimura  H, Yasuda  N, Harada  S, Yamada  K, Suzuki  M, et al. Development of a specific cytolethal distending toxin (cdt) gene (Eacdt)-based PCR assay for the detection of Escherichia albertii. Diagn Microbiol Infect Dis. 2019;95:11924. DOIPubMed
  5. Ooka  T, Ogura  Y, Katsura  K, Seto  K, Kobayashi  H, Kawano  K, et al. Defining the genome features of Escherichia albertii, an emerging enteropathogen closely related to Escherichia coli. Genome Biol Evol. 2015;7:31709.PubMed
  6. Ooka  T, Seto  K, Kawano  K, Kobayashi  H, Etoh  Y, Ichihara  S, et al. Clinical significance of Escherichia albertii. Emerg Infect Dis. 2012;18:48892. DOIPubMed
  7. Lima  MP, Yamamoto  D, Santos  ACM, Ooka  T, Hernandes  RT, Vieira  MAM, et al. Phenotypic characterization and virulence-related properties of Escherichia albertii strains isolated from children with diarrhea in Brazil. Pathog Dis. 2019;77:ftz014. DOIPubMed
  8. Hinenoya  A, Shima  K, Asakura  M, Nishimura  K, Tsukamoto  T, Ooka  T, et al. Molecular characterization of cytolethal distending toxin gene-positive Escherichia coli from healthy cattle and swine in Nara, Japan. BMC Microbiol. 2014;14:97. DOIPubMed
  9. Oaks  JL, Besser  TE, Walk  ST, Gordon  DM, Beckmen  KB, Burek  KA, et al. Escherichia albertii in wild and domestic birds. Emerg Infect Dis. 2010;16:63846. DOIPubMed
  10. Hagiwara  K, Matoba  Y, Asakawa  M. Borna disease virus in Raccoons (Procyon lotor) in Japan. J Vet Med Sci. 2009;71:100915. DOIPubMed
  11. Hattori  K, Donomoto  T, Manchanayake  T, Shibahara  T, Sasai  K, Matsubayashi  M. First surveillance and molecular identification of the Cryptosporidium skunk genotype and Cryptosporidium parvum in wild raccoons (Procyon lotor) in Osaka, Japan. Parasitol Res. 2018;117:366974. DOIPubMed
  12. Lee  K, Iwata  T, Nakadai  A, Kato  T, Hayama  S, Taniguchi  T, et al. Prevalence of Salmonella, Yersinia and Campylobacter spp. in feral raccoons (Procyon lotor) and masked palm civets (Paguma larvata) in Japan. Zoonoses Public Health. 2011;58:42431. DOIPubMed
  13. Beltrán-Beck  B, García  FJ, Gortázar  C. Raccoons in Europe: disease hazards due to the establishment of an invasive species. Eur J Wildl Res. 2012;58:515. DOI
  14. Brandal  LT, Tunsjø  HS, Ranheim  TE, Løbersli  I, Lange  H, Wester  AL. Shiga toxin 2a in Escherichia albertii. J Clin Microbiol. 2015;53:14545. DOIPubMed
  15. Lindsey  RL, Rowe  LA, Batra  D, Smith  P, Strockbine  NA. PacBiol genome sequences of eight Escherichia albertii strains isolated from humans in the United States. Microbiol Resour Announc. 2019;8:e01663-18. DOIPubMed

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Figure
Tables

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Cite This Article

DOI: 10.3201/eid2606.191436

Original Publication Date: May 04, 2020

Table of Contents – Volume 26, Number 6—June 2020

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Shinji Yamasaki, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58, Rinku ourai-kita, Izumisano, Osaka 598-8531, Japan

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Page created: May 19, 2020
Page updated: May 19, 2020
Page reviewed: May 19, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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