Volume 26, Number 6—June 2020
Dispatch
Prevalence of Escherichia albertii in Raccoons (Procyon lotor), Japan
Atsushi Hinenoya, Keigo Nagano, Sharda P. Awasthi, Noritoshi Hatanaka, and Shinji Yamasaki
Table 2
Stx2 production by stx2f gene-positive Escherichia albertii raccoon strains.
| Species |
Strains |
Toxin gene |
Mitomycin C |
Toxin titer* |
Neutralization† |
| E. albertii | RAC199 | stx2f | Negative | 4 | Yes |
| Positive | 512 | Yes | |||
| RAC247 | stx2f | Negative | 32 | Yes | |
| Positive | 1,024 | Yes | |||
| AKT5 | stx2f | Negative | 512 | Yes | |
| Positive |
32,768 |
Yes |
|||
| E. coli | Sakai | stx1, stx2a | Negative | 256 | Not done |
| Positive | 16,384 | Not done | |||
| C600 | None | Negative | <1 | Not done | |
| Positive |
<1 |
Not done |
|||
| *Reciprocal of highest dilution that resulted in death in >50% of cells is shown as toxin titer. †Neutralization of cytotoxic effect by anti-Stx2f rabbit serum. Filtrated culture supernatants in LB-broth with and without mitomycin C were used as toxin samples. | |||||
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Page updated: May 19, 2020
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