Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 26, Number 7—July 2020
Dispatch

Carbapenem Resistance Conferred by OXA-48 in K2-ST86 Hypervirulent Klebsiella pneumoniae, France

Racha BeyrouthyComments to Author , Guillaume Dalmasso, Aurélien Birer, Frédéric Robin, and Richard Bonnet
Author affiliations: Institut National de la Santé et de la Recherche Médicale, Clermont-Ferrand, France (R. Beyrouthy, G. Dalmasso, F. Robin, R. Bonnet); Centre National de Référence de la Résistance aux Antibiotiques, Clermont-Ferrand (R. Beyrouthy, A. Birer, F. Robin, R. Bonnet); Centre Hospitalier Universitaire, Clermont-Ferrand (R. Beyrouthy, F. Robin, R. Bonnet); Institut National de la Recherche Agronomique (USC-2018), Clermont-Ferrand (R. Beyrouthy, F. Robin, G. Dalmasso, R. Bonnet) ; Université Clermont Auvergne, Clermont-Ferrand (G. Dalmasso, F. Robin, R. Bonnet)

Main Article

Figure 1

Comparison of pVIR-Kpn2166 and pVIR-Kpn154 Klebsiella pneumoniae isolates from 2 patients in France (bold) with 16 hypervirulent K. pneumoniae virulence plasmids recovered from the PATRIC database (http://www.patricbrc.org). A) Single-nucleotide polymorphism–based phylogenetic tree built by RaxML from an alignment generated by Burrows-Wheeler Aligner and filtered to remove recombination using Gubbins as previously described (9). The ST and the geographic origin of bacterial hosts are shown. Scal

Figure 1. Comparison of pVIR-Kpn2166 and pVIR-Kpn154 Klebsiella pneumoniae isolates from 2 patients in France (bold) with 16 hypervirulent K. pneumoniae virulence plasmids recovered from the PATRIC database (http://www.patricbrc.org). A) Single-nucleotide polymorphism–based phylogenetic tree built by RaxML from an alignment generated by Burrows-Wheeler Aligner and filtered to remove recombination using Gubbins as previously described (9). The ST and the geographic origin of bacterial hosts are shown. Scale bar indicates mean number of nucleotide substitutions per site. B) Synteny analysis of hypervirulent K. pneumoniae virulence plasmids based on data from blastn (https://blast.ncbi.nlm.nih.gov/Blast.cgi). Virulence-based synteny groups are indicated and the operons encoding the virulence factors, virulence synteny groups of plasmids and the ST and the geographic origin of bacterial hosts. Scale bar indicates kbp. C) Comparison of the details of rearrangements observed in pVIR-Kpn2166 and pVIR-Kpn154 and in pLVPK. Scale bar indicates kbp. ST, sequence type.

Main Article

References
  1. Meatherall  BL, Gregson  D, Ross  T, Pitout  JDD, Laupland  KB. Incidence, risk factors, and outcomes of Klebsiella pneumoniae bacteremia. Am J Med. 2009;122:866–73. DOIPubMedGoogle Scholar
  2. Wyres  KL, Wick  RR, Judd  LM, Froumine  R, Tokolyi  A, Gorrie  CL, et al. Distinct evolutionary dynamics of horizontal gene transfer in drug resistant and virulent clones of Klebsiella pneumoniae. PLoS Genet. 2019;15:e1008114. DOIPubMedGoogle Scholar
  3. Prokesch  BC, TeKippe  M, Kim  J, Raj  P, TeKippe  EME, Greenberg  DE. Primary osteomyelitis caused by hypervirulent Klebsiella pneumoniae. Lancet Infect Dis. 2016;16:e190–5. DOIPubMedGoogle Scholar
  4. Shon  AS, Bajwa  RPS, Russo  TA. Hypervirulent (hypermucoviscous) Klebsiella pneumoniae: a new and dangerous breed. Virulence. 2013;4:107–18. DOIPubMedGoogle Scholar
  5. Lee  CR, Lee  JH, Park  KS, Jeon  JH, Kim  YB, Cha  CJ, et al. Antimicrobial resistance of hypervirulent Klebsiella pneumoniae: epidemiology, hypervirulence-associated determinants, and resistance mechanisms. Front Cell Infect Microbiol. 2017;7:483. DOIPubMedGoogle Scholar
  6. Lam  MMC, Wick  RR, Wyres  KL, Gorrie  CL, Judd  LM, Jenney  AWJ, et al. Genetic diversity, mobilisation and spread of the yersiniabactin-encoding mobile element ICEKp in Klebsiella pneumoniae populations. Microb Genom. 2018;4. DOIPubMedGoogle Scholar
  7. Villa  L, Feudi  C, Fortini  D, García-Fernández  A, Carattoli  A. Genomics of KPC-producing Klebsiella pneumoniae sequence type 512 clone highlights the role of RamR and ribosomal S10 protein mutations in conferring tigecycline resistance. Antimicrob Agents Chemother. 2014;58:1707–12. DOIPubMedGoogle Scholar
  8. Chen  YT, Chang  HY, Lai  YC, Pan  CC, Tsai  SF, Peng  HL. Sequencing and analysis of the large virulence plasmid pLVPK of Klebsiella pneumoniae CG43. Gene. 2004;337:189–98. DOIPubMedGoogle Scholar
  9. Beyrouthy  R, Barets  M, Marion  E, Dananché  C, Dauwalder  O, Robin  F, et al. Novel enterobacter lineage as leading cause of nosocomial outbreak involving carbapenemase-producing strains. Emerg Infect Dis. 2018;24:1505–15. DOIPubMedGoogle Scholar
  10. Beyrouthy  R, Robin  F, Cougnoux  A, Dalmasso  G, Darfeuille-Michaud  A, Mallat  H, et al. Chromosome-mediated OXA-48 carbapenemase in highly virulent Escherichia coli. J Antimicrob Chemother. 2013;68:1558–61. DOIPubMedGoogle Scholar
  11. Russo  TA, Olson  R, Fang  CT, Stoesser  N, Miller  M, MacDonald  U, et al. Identification of biomarkers for differentiation of hypervirulent Klebsiella pneumoniae from classical K. pneumoniae. J Clin Microbiol. 2018;56:1–12. DOIPubMedGoogle Scholar
  12. Hsu  CR, Lin  TL, Chen  YC, Chou  HC, Wang  JT. The role of Klebsiella pneumoniae rmpA in capsular polysaccharide synthesis and virulence revisited. Microbiology. 2011;157:3446–57. DOIPubMedGoogle Scholar
  13. Mataseje  LF, Boyd  DA, Mulvey  MR, Longtin  Y. Two hypervirulent Klebsiella pneumoniae isolates producing a blaKPC-2 carbapenemase from a Canadian patient. Antimicrob Agents Chemother. 2019;63:1–4. DOIPubMedGoogle Scholar
  14. Karlsson  M, Stanton  RA, Ansari  U, McAllister  G, Chan  MY, Sula  E, et al. Identification of a carbapenemase-producing hypervirulent Klebsiella pneumoniae isolate in the United States. Antimicrob Agents Chemother. 2019;63:1–6. DOIPubMedGoogle Scholar
  15. Roulston  KJ, Bharucha  T, Turton  JF, Hopkins  KL, Mack  DJF. A case of NDM-carbapenemase-producing hypervirulent Klebsiella pneumoniae sequence type 23 from the UK. JMM Case Rep. 2018;5:e005130. DOIPubMedGoogle Scholar

Main Article

Page created: April 10, 2020
Page updated: June 18, 2020
Page reviewed: June 18, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external