Volume 26, Number 7—July 2020
Research Letter
Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
Jesse J. Waggoner
, Victoria Stittleburg, Renee Pond, Youssef Saklawi, Malaya K. Sahoo, Ahmed Babiker, Laila Hussaini, Colleen S. Kraft, Benjamin A. Pinsky, Evan J. Anderson, and Nadine Rouphael
, Victoria Stittleburg, Renee Pond, Youssef Saklawi, Malaya K. Sahoo, Ahmed Babiker, Laila Hussaini, Colleen S. Kraft, Benjamin A. Pinsky, Evan J. Anderson, and Nadine Rouphael
Table
Comparison of cycle threshold results for 27 clinical samples tested in triplex real-time reverse transcription PCR and singleplex PCR for severe acute respiratory syndrome coronavirus 2
| Sample | Triplex real-time reverse transcription PCR |
Singleplex reactions |
||||
|---|---|---|---|---|---|---|
| N2 target | E target | RNase P | N2 target | E target | ||
| No template control | ND | ND | ND | ND | ND | |
| Positive control, RNA† | 29.9 | 32.0 | ND | 30.7 | 33.0 | |
| CoV 01 | 19.0 | 23.1 | 21.0 | 19.2 | 23.1 | |
| CoV 02 | ND | ND | 22.1 | ND | ND | |
| CoV 03 | ND | ND | 22.0 | ND | ND | |
| CoV 04 | ND | ND | 21.9 | ND | ND | |
| CoV 05 | ND | ND | 22.4 | ND | ND | |
| CoV 06 | ND | ND | 21.4 | ND | ND | |
| CoV 07 | ND | ND | 21.6 | ND | ND | |
| CoV 08 | 26.1 | 28.0 | 25.5 | 26.1 | 27.7 | |
| CoV 09 | 20.1 | 22.0 | 22.5 | 20.3 | 22.2 | |
| CoV 10 | ND | ND | 21.1 | ND | ND | |
| CoV 11 | ND | ND | 22.0 | ND | ND | |
| CoV 12 | ND | ND | 20.5 | ND | ND | |
| CoV 13 | ND | ND | 20.9 | ND | ND | |
| CoV 14 | ND | ND | 24.6 | ND | ND | |
| CoV 15 | ND | ND | 28.7 | ND | ND | |
| CoV 16 | 14.6 | 16.3 | 22.0 | 14.9 | 16.5 | |
| CoV 17 | ND | ND | 27.6 | 44.8 | ND | |
| CoV 18 | 33.1 | 35.7 | 22.5 | 31.0 | 32.9 | |
| CoV 19 | ND | ND | 28.2 | ND | ND | |
| CoV 20 | 18.6 | 21.3 | 22.5 | 18.9 | 21.5 | |
| CoV 21 | 33.4 | 34.2 | 25.1 | 30.6 | 33.2 | |
| CoV 22 | ND | ND | 21.1 | ND | ND | |
| CoV 23 | 28.9 | 31.3 | 21.3 | 28.2 | 30.4 | |
| CoV 24 | 30.0 | 33.4 | 21.6 | 29.1 | 31.6 | |
| CoV 25 | 9.8 | 13.2 | 20.6 | 9.8 | 13.1 | |
| CoV 26 | ND | ND | 21.5 | ND | ND | |
| CoV 27 | ND | ND | 24.6 | ND | ND | |
*CoV, coronavirus; E, envelope gene; N2, nucleocapsid 2 gene; ND, not detected; RNase P, ribonuclease P.
†Strain BetaCoV/Germany/BavPat1/2020p.1.
Page created: April 15, 2020
Page updated: June 18, 2020
Page reviewed: June 18, 2020
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