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Volume 26, Number 8—August 2020
Dispatch

Imported Monkeypox, Singapore

Sarah Ee Fang Yong, Oon Tek Ng, Zheng Jie Marc Ho, Tze Minn Mak, Kalisvar Marimuthu, Shawn Vasoo, Tsin Wen Yeo, Yi Kai Ng, Lin Cui, Zannatul Ferdous, Po Ying Chia, Bryan Jun Wei Aw, Charmaine Malenab Manauis, Constance Khia Ki Low, Guanhao Chan, Xinyi Peh, Poh Lian Lim, Li Ping Angela Chow, Monica Chan, Vernon Jian Ming Lee, Raymond Tzer Pin Lin, Mok Kwee Derrick HengComments to Author , and Yee Sin LeoComments to Author 
Author affiliations: Ministry of Health Singapore, Singapore (S.E.F. Yong, Z.J.M. Ho, C.K.K. Low, G. Chan, X. Peh, V.J.M. Lee, M.K.D. Heng); National Centre for Infectious Diseases, Singapore (O.T. Ng, T.M. Mak, K. Marimuthu, S. Vasoo, T.W. Yeo, Y.K. Ng, L. Cui, Z. Ferdous, P.Y. Chia, B.J.W. Aw, C.M. Manauis, P.L. Lim, L.P.A. Chow, M. Chan, R.T.P. Lin, Y.S. Leo); National Public Health Laboratory, Singapore (T.M. Mak, Y.K. Ng, L. Cui, R.T.P. Lin); Tan Tock Seng Hospital, Singapore (O.T. Ng, K. Marimuthu, S. Vasoo, T.W. Yeo, Z. Ferdous, P.Y. Chia, B.J.W. Aw, C.M. Manauis, P.L. Lim, L.P.A. Chow, Y.S. Leo); National University Health System, Singapore (S.E.F. Yong); Nanyang Technological University, Singapore (O.T. Ng, P.Y. Chia, P.L. Lim, Y.S. Leo); National University of Singapore, Singapore (K. Marimuthu, P.L. Lim, Y.S. Leo)

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Figure 2

Transmission electron microscopy and maximum-likelihood phylogenetic tree of monkeypox virus in 38-year-old man, Singapore, 2019. A, B) Multiple brick-shaped particles, ranging from 230–290 nm by 130–240 nm, were observed from vesicle fluid under transmission electron microscopy. Tubular structures were observed with phosphotungstic acid stain (A), and a central ring-like depression was observed with gadolinium acetate stain (B). C) Phylogeny of monkeypox sequences, with the patient’s monkeypox

Figure 2. Transmission electron microscopy and maximum-likelihood phylogenetic tree of monkeypox virus in 38-year-old man, Singapore, 2019. A, B) Multiple brick-shaped particles, ranging from 230–290 nm by 130–240 nm, were observed from vesicle fluid under transmission electron microscopy. Tubular structures were observed with phosphotungstic acid stain (A), and a central ring-like depression was observed with gadolinium acetate stain (B). C) Phylogeny of monkeypox sequences, with the patient’s monkeypox strain in bold. All strains are identified by GenBank accession number, location, and year. The evolutionary relationships between monkeypox strains was determined based on 184,338 bases within the central core region of the monkeypox genome. The maximum-likelihood tree was created using RAxML (10) with γ-distributed rate differences and 1,000 bootstrap validation. Only bootstrap values >70% are displayed on the internal branches. Central African and West African clades are indicated. Scale bar indicates genetic distance between sequences.

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Page updated: July 18, 2020
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