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Volume 27, Number 2—February 2021

Borrelia burgdorferi Sensu Stricto DNA in Field-Collected Haemaphysalis longicornis Ticks, Pennsylvania, United States

Keith J. PriceComments to Author , Christine B. Graham, Bryn J. Witmier, Holly A. Chapman, Brooke L. Coder, Christian N. Boyer, Erik Foster, Sarah E. Maes, Ying Bai, Rebecca J. Eisen, and Andrew D. Kyle
Author affiliations: Pennsylvania Department of Environmental Protection, Harrisburg, Pennsylvania, USA (K.J. Price, B.J. Witmier, H.A. Chapman, B.L. Coder, C.N. Boyer, A.D. Kyle); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (C.B. Graham, E. Foster, S.E. Maes, Y. Bai, R.J. Eisen)

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Pathogen targets included in real-time PCR testing of individual Haemaphysalis longicornis ticks, Pennsylvania, USA *†

PCR target Pathogen

Borrelia burgdorferi sensu stricto B. mayonii B. miyamotoi Babesia microti Reference
Borrelia 16S rDNA NA (7)
B. burgdorferi sensu lato fliD NA NA (8)
B. burgdorferi sensu stricto oppA2 NA NA NA (9)
B. mayonii oppA2 NA NA NA (9)
Borrelia miyamotoi purB NA NA NA (9)
B. miyamotoi glpQ NA NA NA (9)
B. microti sa1 NA NA NA (10)
B. microti 18S rDNA NA NA NA (10)

*fliD, flagellin gene; NA, not applicable; oppA2, oligopeptide permease periplasmic A2 gene; purB, adenylosuccinate lyase gene; glpQ, glycerophosphodiester phosphodiesterase gene; sa1, secreted antigen 1 gene.
†A sample was considered positive for a pathogen only if it was positive for all associated targets.
‡Targets associated with each pathogen.

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Page created: December 04, 2020
Page updated: January 24, 2021
Page reviewed: January 24, 2021
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