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Volume 27, Number 2—February 2021
Research

Spread of Multidrug-Resistant Rhodococcus equi, United States

Sonsiray Álvarez-NarváezComments to Author , Steeve Giguère1, Noah Cohen, Nathan Slovis, and José A. Vázquez-BolandComments to Author 
Author affiliations: University of Georgia, Athens, Georgia, USA (S. Álvarez-Narváez, S. Giguère); Texas A&M University, College Station, Texas, USA (N. Cohen); Hagyard Equine Medical Institute, Lexington, Kentucky, USA (N. Slovis); University of Edinburgh, Edinburgh, Scotland, UK (J.A. Vázquez-Boland)

Main Article

Figure 1

Spread and phylogenetic relationships of MDR Rhodococcus equi, United States. Phylogenetic tree of 93 R. equi isolates based on core-genome single-nucleotide polymorphism analysis by using ParSNP (18). The genomes analyzed are from 58 erm(46)-positive MR isolates, 24 control-susceptible isolates from same period and geographic origins, and 23 isolates representative of the genomic diversity of R. equi, including the reference genome 103S (33) and the type strain DSM 20307T (Appendix Table 1). Tip labels show year of collection and resistance phenotype for the 2001–2017 equine clinical isolates analyzed (the 50 genomes determined in this study are shown in bold, and other genomes are from previous study [15]). Red indicates MDR 2287 clonal complex, violet indicates novel MDR G2016 clone, blue indicates genetically diverse MR isolates recovered from a farm in Louisiana during 2015–2017 (MDR 2287 isolate from which they likely acquired the pRErm46 plasmid is indicated by an asterisk), and green indicates an RR isolate (rpoB S531K mutation). pRErm46 carriage status is indicated by symbols. Tree graph constructed with FigTree (http://tree.bio.ed.ac.uk/software/figtree). MDR, multidrug-resistant; MR, macrolide-resistant; MRR, dual macrolide/rifampin resistant; RR, rifampin-resistant.

Figure 1. Spread and phylogenetic relationships of MDR Rhodococcus equi, United States. Phylogenetic tree of 93 R. equi isolates based on core-genome single-nucleotide polymorphism analysis by using ParSNP (18). The genomes analyzed are from 58 erm(46)-positive MR isolates, 24 control-susceptible isolates from same period and geographic origins, and 23 isolates representative of the genomic diversity of R. equi, including the reference genome 103S (33) and the type strain DSM 20307T (Appendix Table 1). Tip labels show year of collection and resistance phenotype for the 2001–2017 equine clinical isolates analyzed (the 50 genomes determined in this study are shown in bold, and other genomes are from previous study [15]). Red indicates MDR 2287 clonal complex, violet indicates novel MDR G2016 clone, blue indicates genetically diverse MR isolates recovered from a farm in Louisiana during 2015–2017 (MDR 2287 isolate from which they likely acquired the pRErm46 plasmid is indicated by an asterisk), and green indicates an RR isolate (rpoB S531K mutation). pRErm46 carriage status is indicated by symbols. Tree graph constructed with FigTree (http://tree.bio.ed.ac.uk/software/figtree). MDR, multidrug-resistant; MR, macrolide-resistant; MRR, dual macrolide/rifampin resistant; RR, rifampin-resistant.

Main Article

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1Deceased.

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