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Volume 27, Number 4—April 2021
Research Letter

Increased Likelihood of Detecting Ebola Virus RNA in Semen by Using Sample Pelleting

Courtney M. Bozman, Mosoka Fallah, Michael C. Sneller, Catherine Freeman, Lawrence S. Fakoli, Bode I. Shobayo, Bonnie Dighero-Kemp, Cavan S. Reilly, Jens H. Kuhn, Fatorma Bolay, Elizabeth Higgs, and Lisa E. HensleyComments to Author 
Author affiliations: National Institutes of Health, Frederick, Maryland, USA (C.M. Bozman, B. Dighero-Kemp, J.H. Kuhn, L.E. Hensley); National Public Health Institute of Liberia, Monrovia, Liberia (M. Fallah, C. Freeman, L.S. Fakoli III, B.I. Shobayo, F. Bolay); National Institutes of Health, Bethesda, Maryland, USA (M.C. Sneller, E. Higgs); University of Minnesota, Minneapolis, Minnesota, USA (C.S. Reilly)

Main Article

Table

Number of positive samples detected in whole semen samples versus pelleted semen samples for detection of Ebola virus RNA*

Method: replicate No. samples No. invalid samples No. (%) Ebola virus RNA positive samples
Whole sample: A 1,661 66 50 (3.1)
Whole sample: B 1,661 112 45 (2.9)
Pellet sample: A 1,661 85 84 (5.3)
Pellet sample: B 1,661 69 73 (4.6)

*Also included are no. invalid samples (i.e., those that did not pass 1 or both of the controls (sample processing control and probe check control).

Main Article

Page created: January 15, 2021
Page updated: March 19, 2021
Page reviewed: March 19, 2021
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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