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Volume 27, Number 5—May 2021
Research

Monitoring SARS-CoV-2 Circulation and Diversity through Community Wastewater Sequencing, the Netherlands and Belgium

Ray Izquierdo-Lara, Goffe Elsinga, Leo Heijnen, Bas B. Oude Munnink, Claudia M.E. Schapendonk, David Nieuwenhuijse, Matthijs Kon, Lu Lu, Frank M. Aarestrup, Samantha Lycett, Gertjan Medema1, Marion P.G. Koopmans1, and Miranda de Graaf1Comments to Author 
Author affiliations: Erasmus University Medical Center, Rotterdam, the Netherlands (R. Izquierdo-Lara, B.B. Oude Munnink, C.M.E. Schapendonk, D. Nieuwenhuijse, M. Kon, M.P.G. Koopmans, M. de Graaf); KWR Water Research Institute, Nieuwegein, the Netherlands (G. Elsinga, L. Heijnen, G. Medema); University of Edinburgh, Edinburgh, Scotland, UK (L. Lu, S. Lycett); Technical University of Denmark, Kongens Lyngby, Denmark (F.M. Aarestrup)

Main Article

Figure 2

Phylogenetic analysis of severe acute respiratory syndrome coronavirus 2 genome consensus sequences detected in sewage samples, the Netherlands and Belgium. A) The Netherlands subsample dataset; B) global subsample dataset. Lines with dots in green indicate samples sequenced in this study. Clades (19A, 19B, 20A, 20B, and 20C) were assigned by using the Nextclade tool (https://clades.nextstrain.org). For the global subsample tree, samples in orange indicate the Netherlands sequences. Samples in purple indicate Belgium sequences. Scale bars indicate inferred number of nucleotide substitutions per site.

Figure 2. Phylogenetic analysis of severe acute respiratory syndrome coronavirus 2 genome consensus sequences detected in sewage samples, the Netherlands and Belgium. A) The Netherlands subsample dataset; B) global subsample dataset. Lines with dots in green indicate samples sequenced in this study. Clades (19A, 19B, 20A, 20B, and 20C) were assigned by using the Nextclade tool (https://clades.nextstrain.org). For the global subsample tree, samples in orange indicate the Netherlands sequences. Samples in purple indicate Belgium sequences. Scale bars indicate inferred number of nucleotide substitutions per site.

Main Article

1These senior authors contributed equally to this article.

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Page updated: April 20, 2021
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